The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
State‐of‐the‐art in phosphoproteomics
Open accessAbstract
Presently, phosphorylation of proteins is the most studied and best understood PTM. However, the analysis of phosphoproteins and phosphopeptides is still one of the most challenging tasks in contemporary proteome research. Since not every phosphoprotein is accessible by a certain method and identification of the phosphorylated amino acid residue is required in the majority of cases, various strategies for the detection and localization of phosphorylations have been developed. Identification and localization of protein phosphorylations is mostly done by MS nowadays but phosphoproteins and ‐peptides are often suppressed in comparison to the unphosphorylated species if measured in complex mixtures. Thus, the isolation of pure phosphopeptide samples is a main task. This review gives an overview over the most frequently used methods in isolation and detection of phosphoproteins and ‐peptides such as specific enrichment or separation strategies as well as the localization of the phosphorylated residues by various mass spectrometric techniques.
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Ong, S.-E., Blagoev, B., Kratchmarova, I., Kristensen, D. B., Steen, H., Pandey, A., & Mann, M. (2002) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m200025-mcp200 · Cited by 5,574
Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry
Syka, J. E. P., Coon, J. J., Schroeder, M. J., Shabanowitz, J., & Hunt, D. F. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402700101 · Cited by 2,370
Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS
Gerber, S. A., Rush, J., Stemman, O., Kirschner, M. W., & Gygi, S. P. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0832254100 · Cited by 1,733
Cited by
The utility of ETD mass spectrometry in proteomic analysis
Mikesh, L. M., Ueberheide, B., Chi, A., Coon, J. J., Syka, J. E. P., Shabanowitz, J., & Hunt, D. F. (2006) · Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2006.10.003 · Cited by 535
Analytical strategies for phosphoproteomics
Thingholm, T. E., Jensen, O. N., & Larsen, M. R. (2009) · PROTEOMICS · doi:10.1002/pmic.200800454 · Cited by 477
Phosphopeptide fragmentation and analysis by mass spectrometry
Boersema, P. J., Mohammed, S., & Heck, A. J. R. (2009) · Journal of Mass Spectrometry · doi:10.1002/jms.1599 · Cited by 362
Pröfrock, D., & Prange, A. (2012) · Applied Spectroscopy · doi:10.1366/12-06681 · Cited by 329
Guidelines for the next 10 years of proteomics
Wilkins, M. R., Appel, R. D., Van Eyk, J. E., Chung, M. C. M., Görg, A., Hecker, M., Huber, L. A., Langen, H., Link, A. J., Paik, Y., et al. (2006) · PROTEOMICS · doi:10.1002/pmic.200500856 · Cited by 307
Metadata
- Authors
- Reinders, J., & Sickmann, A.
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2005-10-27
- Volume
- 5
- Issue
- 16
- Pages
- 4052-4061
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- OpenAlex
- W2002642674
- Cited by
- 346
- References
- 3
- FWCI
- 18.65
- Type
- journal article
- Language
- en
- Open access
- bronze
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