The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Comparative LC‐MS: A landscape of peaks and valleys
Open accessAbstract
Quantitative proteomics approaches using stable isotopes are well‐known and used in many labs nowadays. More recently, high resolution quantitative approaches are reported that rely on LC‐MS quantitation of peptide concentrations by comparing peak intensities between multiple runs obtained by continuous detection in MS mode. Characteristic of these comparative LC‐MS procedures is that they do not rely on the use of stable isotopes; therefore the procedure is often referred to as label‐free LC‐MS. In order to compare at comprehensive scale peak intensity data in multiple LC‐MS datasets, dedicated software is required for detection, matching and alignment of peaks. The high accuracy in quantitative determination of peptide abundancies provides an impressive level of detail. This approach also requires an experimental set‐up where quantitative aspects of protein extraction and reproducible separation conditions need to be well controlled. In this paper we will provide insight in the critical parameters that affect the quality of the results and list an overview of the most recent software packages that are available for this procedure.
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“This is especially so for the in vivo labeled samples, where the mass shift of the peptide is dependent on the number of incorporated isotope molecules, which is dictated by the peptide sequence [26–31].”
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Cited by
Quantitative mass spectrometry in proteomics: critical review update from 2007 to the present
Bantscheff, M., Lemeer, S., Savitski, M. M., & Kuster, B. (2012) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-012-6203-4 · Cited by 801
Mass Spectrometry-Based Label-Free Quantitative Proteomics
Zhu, W., Smith, J. W., & Huang, C.-M. (2010) · Journal of Biomedicine and Biotechnology · doi:10.1155/2010/840518 · Cited by 629
Panel), E. P. O. G. M. O. (. (2010) · EFSA Journal · doi:10.2903/j.efsa.2010.1700 · Cited by 608
Role of spectral counting in quantitative proteomics
Lundgren, D. H., Hwang, S.-I., Wu, L., & Han, D. K. (2010) · Expert Review of Proteomics · doi:10.1586/epr.09.69 · Cited by 428
Comprehensive Characterization of Extracellular Herpes Simplex Virus Type 1 Virions
Loret, S., Guay, G., & Lippé, R. (2008) · Journal of Virology · doi:10.1128/jvi.00904-08 · Cited by 365
Metadata
- Authors
- America, A. H. P., & Cordewener, J. H. G.
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2008-02
- Volume
- 8
- Issue
- 4
- Pages
- 731-749
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- OpenAlex
- W1991777486
- Cited by
- 191
- References
- 10
- FWCI
- 13.99
- Type
- journal article
- Language
- en
- Open access
- green
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