Cited by 1,975Gene expression and cancer classificationBioinformatics and Genomic NetworksGenomics and Phylogenetic Studies

Microarray data normalization and transformation

Quackenbush, John·2002·Nature Genetics

Abstract

Underlying every microarray experiment is an experimental question that one would like to address. Finding a useful and satisfactory answer relies on careful experimental design and the use of a variety of data-mining tools to explore the relationships between genes or reveal patterns of expression. While other sections of this issue deal with these lofty issues, this review focuses on the much more mundane but indispensable tasks of 'normalizing' data from individual hybridizations to make meaningful comparisons of expression levels, and of 'transforming' them to select genes for further analysis and data mining.

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Crossref · 10.1038/ng1032

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  • A robust local regression procedure (LOWESS) was performed to remove systematic variations in measured gene expression levels so differences could be distinguished accurately and precisely.

    “A robust local regression procedure (LOWESS) was performed to remove the systematic variations in the measured gene expression levels so that differences in expression across the samples could be distinguished accurately and precisely (21).”

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Authors
Quackenbush, J.
Corresponding author
Quackenbush, John
Venue
Nature Genetics
Published
2002-12
Volume
32
Issue
S4
Pages
496-501
ISSN
  • 1061-4036 (ISSN-L)
  • 1546-1718
OpenAlex
W2133111499
Cited by
1,975
References
19
Citations by year
2026: 25 · 2025: 38 · 2024: 38 · 2023: 45 · 2022: 53 · 2021: 53 · 2020: 61 · 2019: 49 · 2018: 51 · 2017: 51 · 2016: 61 · 2015: 68 · 2014: 75 · 2013: 87 · 2012: 90
FWCI
43.10
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Genetics (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords
microarray normalization, data transformation, gene expression data, nucleic acid hybridization, gene selection, data mining
MeSH terms
Animals, Data Interpretation, Statistical, Forecasting, Humans, Research Design, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling

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