Changes in the proteome of Salmonella enterica serovar Thompson as stress adaptation to sublethal concentrations of thymol
Abstract
Thymol is a natural biocide and component of some essential oils from herbs. Its inhibitory effect on the growth of different microorganisms is well documented. The precise targets of the antibacterial action of thymol is not yet been fully established, the action seems to take place in different ways. The strain Salmonella enterica serovar Thompson MCV1 was grown in the presence of a sublethal concentration (0.01%) of thymol. The proteins extracted from treated and untreated cells were subjected to 2-D PAGE, followed by in-gel spot digestion and subsequent MALDI-TOF analysis. The analysis of gels showed many proteins that were either upregulated or downregulated by the presence of thymol, with significant changes in proteins belonging to different functional classes. In particular, the thioredoxin-1 was not expressed in the treated cells, indicating that its absence could be a consequence of the stress caused by the presence of thymol. On the other hand, different chaperon proteins were upregulated or de novo synthesis such as GroEL and DnaK, key proteins in the protection mechanism toward thermal stress. Outer membrane proteins were upregulated in treated cells; indeed the bacterial envelope stress response is trigged by the accumulation of misfolded outer membrane proteins. Moreover, the thymol seems to impair the citrate metabolic pathway, as well as many enzymes involved in the synthesis of ATP. Definitely, thymol plays a role in altering very different pathways of cell metabolism.
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S. enterica serovar Thompson MCV1 grown in the presence of a sub-lethal concentration of thymol showed a different proteomic profile compared with the control.
“S. enterica serovar Thompson MCV1 grown in the presence of a sub-lethal concentration of thymol showed a different proteomic profile compared with the control [80].”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [80]
Proteomic approaches in Salmonella cells treated with thymol confirmed the effect on cell division proteins.
“This was confirmed by proteomic approaches in Salmonella cells treated with thymol [80].”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [80]
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“concentration of thymol showed a different proteomic profile compared with the control [80].”
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- Authors
- Di Pasqua, R., Mamone, G., Ferranti, P., Ercolini, D., & Mauriello, G.
- Affiliations
- Institute of Food Science (IT)
- University of Naples Federico II (IT)
- Corresponding author
- Mauriello, Gianluigi
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2010-02-26
- Volume
- 10
- Issue
- 5
- Pages
- 1040-1049
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- OpenAlex
- W2052512407
- Cited by
- 171
- References
- 29
- Citations by year
- 2026: 4 · 2025: 12 · 2024: 9 · 2023: 9 · 2022: 17 · 2021: 15 · 2020: 16 · 2019: 10 · 2018: 14 · 2017: 11 · 2016: 9 · 2015: 9 · 2014: 8 · 2013: 13 · 2012: 10
- FWCI
- 5.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- PROTEOMICS (publishedVersion)
- PubMed (publishedVersion)
- Funders
- EU project (211638)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- thymol, sublethal concentrations, stress adaptation, proteome changes, thioredoxin-1, molecular chaperones, GroEL, DnaK, outer membrane proteins, envelope stress response, citrate metabolism, ATP production, essential oils, antibacterial activity
- MeSH terms
- Adaptation, Physiological, Bacterial Proteins, Citrates, Fermentation, Heat-Shock Proteins, Stress, Physiological, Thymol, Electrophoresis, Gel, Two-Dimensional, Salmonella enterica, Proteome, Metabolic Networks and Pathways
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