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Cited by 280Essential Oils and Antimicrobial ActivityEscherichia coli research studiesHeat shock proteins research

Carvacrol Induces Heat Shock Protein 60 and Inhibits Synthesis of Flagellin in Escherichia coli O157:H7

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Burt, Sara A., van der Zee, Ruurd, Koets, Ad P., de Graaff, Anko M., van Knapen, Frans, Gaastra, Wim, Haagsman, Henk P., Veldhuizen, Edwin J. A.·2007·Applied and Environmental Microbiology
DOI 10.1128/aem.00340-07PMC PMC1932834
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Abstract

The essential oils of oregano and thyme are active against a number of food-borne pathogens, such as Escherichia coli O157:H7. Carvacrol is one of the major antibacterial components of these oils, and p-cymene is thought to be its precursor in the plant. The effects of carvacrol and p-cymene on protein synthesis in E. coli O157:H7 ATCC 43895 cells were investigated. Bacteria were grown overnight in Mueller-Hinton broth with a sublethal concentration of carvacrol or p-cymene, and their protein compositions were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and confirmed by Western blotting. The presence of 1 mM carvacrol during overnight incubation caused E. coli O157:H7 to produce significant amounts of heat shock protein 60 (HSP60) (GroEL) (P < 0.05) and inhibited the synthesis of flagellin highly significantly (P < 0.001), causing cells to be aflagellate and therefore nonmotile. The amounts of HSP70 (DnaK) were not significantly affected. p-Cymene at 1 mM or 10 mM did not induce HSP60 or HSP70 in significant amounts and did not have a significant effect on flagellar synthesis. Neither carvacrol (0.3, 0.5, 0.8, or 1 mM) nor p-cymene (0.3, 0.5, or 0.8 mM) treatment of cells in the mid-exponential growth phase induced significant amounts of HSP60 or HSP70 within 3 h, although numerical increases of HSP60 were observed. Motility decreased with increasing concentrations of both compounds, but existing flagella were not shed. This study is the first to demonstrate that essential oil components induce HSP60 in bacteria and that overnight incubation with carvacrol prevents the development of flagella in E. coli O157:H7.

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Crossref · 10.1128/aem.00340-07

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  • In many conditions, such as in the presence of sub-lethal concentrations of essential oils or other antimicrobial compounds, microorganisms react by increasing their expression of stress-response proteins to repair damaged proteins.

    “In many conditions, such as in the presence of sub-lethal concentrations of EOs or other antimicrobial compounds, microorganisms react by increasing their expression of the stress-response proteins [25] to repair the damaged proteins [20].”

    From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [25]

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Authors
Burt, S. A., van der Zee, R., Koets, A. P., de Graaff, A. M., van Knapen, F., Gaastra, W., Haagsman, H. P., & Veldhuizen, E. J. A.
Affiliations
  • Institute for Risk Assessment Sciences, Division of Veterinary Public Health
  • Department of Infectious Diseases and Immunology
  • Centre for Cell Imaging, Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
Corresponding author
Burt, Sara A.
ORCID
Venue
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Published
2007-07-15
Volume
73
Issue
14
Pages
4484-4490
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
DOI
10.1128/aem.00340-07
PMC
PMC1932834
OpenAlex
W2167358281
Cited by
280
References
37
Citations by year
2026: 6 · 2025: 17 · 2024: 17 · 2023: 11 · 2022: 24 · 2021: 11 · 2020: 25 · 2019: 17 · 2018: 19 · 2017: 20 · 2016: 24 · 2015: 13 · 2014: 11 · 2013: 19 · 2012: 17
FWCI
11.94
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
Keywords
carvacrol, p-cymene, Escherichia coli O157:H7, heat shock protein 60, GroEL, bacterial motility, HSP70, DnaK, essential oils, oregano, thyme, foodborne pathogens, antibacterial activity, protein synthesis
MeSH terms
Cymenes, Anti-Bacterial Agents, Electrophoresis, Polyacrylamide Gel, Flagella, Flagellin, Locomotion, Microscopy, Interference, Blotting, Western, Chaperonin 60, HSP70 Heat-Shock Proteins, Escherichia coli O157, Proteome, Escherichia coli Proteins, Monoterpenes

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