The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Proteome profiling of exosomes derived from human primary and metastatic colorectal cancer cells reveal differential expression of key metastatic factors and signal transduction components
Open accessAbstract
Exosomes are small extracellular 40–100 nm diameter membrane vesicles of late endosomal origin that can mediate intercellular transfer of RNA s and proteins to assist premetastatic niche formation. Using primary (SW 480) and metastatic (SW 620) human isogenic colorectal cancer cell lines we compared exosome protein profiles to yield valuable insights into metastatic factors and signaling molecules fundamental to tumor progression. Exosomes purified using O pti P rep™ density gradient fractionation were 40–100 nm in diameter, were of a buoyant density ∼1.09 g/mL, and displayed stereotypic exosomal markers TSG 101, A lix, and CD 63. A major finding was the selective enrichment of metastatic factors (MET, S 100 A 8, S 100 A 9, TNC), signal transduction molecules (EFNB 2, JAG 1, SRC, TNIK), and lipid raft and lipid raft‐associated components (CAV 1, FLOT 1, FLOT 2, PROM 1) in exosomes derived from metastatic SW 620 cells. Additionally, using cryo‐electron microscopy, ultrastructural components in exosomes were identified. A key finding of this study was the detection and colocalization of protein complexes EPCAM‐CLDN 7 and TNIK‐RAP 2 A in colorectal cancer cell exosomes. The selective enrichment of metastatic factors and signaling pathway components in metastatic colon cancer cell‐derived exosomes contributes to our understanding of the cross‐talk between tumor and stromal cells in the tumor microenvironment.
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Microenvironmental regulation of metastasis
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Genes that mediate breast cancer metastasis to lung
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Syndecan–syntenin–ALIX regulates the biogenesis of exosomes
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Breast cancer cells produce tenascin C as a metastatic niche component to colonize the lungs
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Amphiregulin exosomes increase cancer cell invasion
Higginbotham, J. N., Demory Beckler, M., Gephart, J. D., Franklin, J. L., Bogatcheva, G., Kremers, G.-J., Piston, D. W., Ayers, G. D., McConnell, R. E., Tyska, M. J., et al. (2011) · Current Biology · doi:10.1016/j.cub.2011.03.043 · Cited by 360
Cited by
The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling
Van Deun, J., Mestdagh, P., Sormunen, R., Cocquyt, V., Vermaelen, K., Vandesompele, J., Bracke, M., De Wever, O., & Hendrix, A. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.24858 · Cited by 938
Haraszti, R. A., Didiot, M., Sapp, E., Leszyk, J., Shaffer, S. A., Rockwell, H. E., Gao, F., Narain, N. R., DiFiglia, M., Kiebish, M. A., et al. (2016) · Journal of Extracellular Vesicles · doi:10.3402/jev.v5.32570 · Cited by 746
Plasma Proteome Database as a resource for proteomics research: 2014 update
Nanjappa, V., Thomas, J. K., Marimuthu, A., Muthusamy, B., Radhakrishnan, A., Sharma, R., Ahmad Khan, A., Balakrishnan, L., Sahasrabuddhe, N. A., Kumar, S., et al. (2014) · Nucleic Acids Research · doi:10.1093/nar/gkt1251 · Cited by 314
Self-citationTGF-β signaling in cancer metastasis
Xie, F., Ling, L., Van Dam, H., Zhou, F., & Zhang, L. (2018) · Acta Biochimica et Biophysica Sinica · doi:10.1093/abbs/gmx123 · Cited by 231
Mukherjee, K., Ghoshal, B., Ghosh, S., Chakrabarty, Y., Shwetha, S., Das, S., & Bhattacharyya, S. N. (2016) · The EMBO Reports · doi:10.15252/embr.201541930 · Cited by 184
Metadata
- Authors
- Ji, H., Greening, D. W., Barnes, T. W., Lim, J. W., Tauro, B. J., Rai, A., Xu, R., Adda, C., Mathivanan, S., Zhao, W., Xue, Y., Xu, T., Zhu, H., & Simpson, R. J.
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2013-05
- Volume
- 13
- Issue
- 10-11
- Pages
- 1672-1686
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- OpenAlex
- W1968542304
- Cited by
- 357
- References
- 7
- FWCI
- 15.87
- Type
- journal article
- Language
- en
- Open access
- green
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- Ji, H., Greening, D. W., Barnes, T. W., Lim, J. W., Tauro, B. J., Rai, A., Xu, R., Adda, C., Mathivanan, S., Zhao, W., Xue, Y., Xu, T., Zhu, H., & Simpson, R. J. (2013). Proteome profiling of exosomes derived from human primary and metastatic colorectal cancer cells reveal differential expression of key metastatic factors and signal transduction components. PROTEOMICS. https://doi.org/10.1002/pmic.201200562
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