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Meet the neighbors: Mapping local protein interactomes by proximity‐dependent labeling with BioID

Varnaitė, Renata, MacNeill, Stuart A.·2016·PROTEOMICS
DOI 10.1002/pmic.201600123PMC PMC5053326
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Abstract

Proximity‐dependent biotin identification (BioID) is a recently developed method that allows the identification of proteins in the close vicinity of a protein of interest in living cells. BioID relies on fusion of the protein of interest with a mutant form of the biotin ligase enzyme BirA (BirA*) that is capable of promiscuously biotinylating proximal proteins irrespective of whether these interact directly or indirectly with the fusion protein or are merely located in the same subcellular neighborhood. The covalent addition of biotin allows the labeled proteins to be purified from cell extracts on the basis of their affinity for streptavidin and identified by mass spectrometry. To date, BioID has been successfully applied to study a variety of proteins and processes in mammalian cells and unicellular eukaryotes and has been shown to be particularly suited to the study of insoluble or inaccessible cellular structures and for detecting weak or transient protein associations. Here, we provide an introduction to BioID, together with a detailed summary of where and how the method has been applied to date, and briefly discuss technical aspects involved in the planning and execution of a BioID study.

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  1. 10.1074/mcp.R115.052902 · DOI
  2. 10.1007/s00018-013-1287-3 · DOI
  3. 10.1074/jbc.M113.529578 · DOI
  4. 10.1126/science.1230593 · DOI · Paper page
  5. 10.1083/jcb.201112098 · DOI · Paper page
  6. Roux K. J., 2013, BioID: a screen for protein–protein interactions, Curr. Protoc. Protein Sci., 74, 19
  7. 10.1016/S0968-0004(99)01438-3 · DOI
  8. 10.1016/S0021-9258(18)93826-3 · DOI
  9. 10.1110/ps.04911804 · DOI
  10. 10.1110/ps.9.8.1530 · DOI
  11. 10.1073/pnas.1406459111 · DOI · Paper page
  12. 10.1016/j.jsb.2011.11.007 · DOI
  13. 10.1146/annurev-biochem-060614-034115 · DOI
  14. 10.1242/jcs.083246 · DOI
  15. 10.7554/eLife.07759 · DOI
  16. 10.1038/srep17186 · DOI
  17. 10.1038/nsmb.2998 · DOI
  18. 10.1038/nrm4062 · DOI
  19. 10.1016/j.cell.2015.10.065 · DOI · Paper page
  20. 10.1016/j.cub.2014.01.067 · DOI
  21. 10.1016/j.cub.2013.06.002 · DOI
  22. 10.1016/bs.mcb.2015.03.016 · DOI
  23. 10.1371/journal.pone.0117074 · DOI
  24. 10.1242/jcs.140475 · DOI
  25. 10.1074/jbc.M113.466193 · DOI
  26. 10.1371/journal.pone.0122886 · DOI
  27. 10.1083/jcb.201304115 · DOI
  28. 10.15252/embj.201592695 · DOI
  29. 10.1126/scisignal.2004712 · DOI
  30. 10.1016/j.devcel.2014.12.009 · DOI
  31. 10.1101/gad.267708.115 · DOI
  32. 10.1073/pnas.0508310102 · DOI
  33. 10.1038/ng1652 · DOI
  34. 10.1038/nrm4063 · DOI
  35. 10.1371/journal.pone.0150239 · DOI
  36. 10.1038/onc.2013.144 · DOI
  37. 10.1074/mcp.M114.045658 · DOI
  38. 10.1073/pnas.1521763113 · DOI
  39. 10.1016/j.bbamcr.2011.06.007 · DOI
  40. 10.1016/j.ccell.2015.05.004 · DOI
  41. 10.1146/annurev-pathol-011110-130237 · DOI
  42. 10.2217/14796694.1.4.521 · DOI
  43. 10.1021/pr500387m · DOI
  44. 10.1038/nrc2231 · DOI · Paper page
  45. 10.1016/j.jprot.2014.09.029 · DOI · Paper page
  46. 10.1016/j.jprot.2014.09.011 · DOI
  47. 10.1016/j.jprot.2013.12.022 · DOI
  48. 10.1016/j.jmb.2016.01.028 · DOI
  49. 10.7554/eLife.05560 · DOI
  50. 10.1074/jbc.M115.694109 · DOI
  51. 10.1091/mbc.E15-04-0219 · DOI
  52. 10.1079/AHR2005100 · DOI
  53. Kono M., 2013, The apicomplexan inner membrane complex, Front. Biosci. (Landmark Ed.), 18, 982
  54. 10.1128/mBio.02357-14 · DOI
  55. 10.1371/journal.ppat.1005268 · DOI
  56. 10.1007/s11010-009-0111-8 · DOI
  57. 10.3390/cells5010013 · DOI
  58. 10.1016/bs.mie.2015.09.007 · DOI
  59. 10.1016/j.tim.2012.11.006 · DOI · Paper page
  60. 10.1128/JVI.03584-14 · DOI
  61. 10.1186/s12985-015-0365-6 · DOI
  62. 10.1371/journal.ppat.1005141 · DOI
  63. 10.1128/JVI.00321-15 · DOI
  64. 10.1093/femspd/ftu007 · DOI
  65. 10.1091/mbc.E14-11-1530 · DOI
  66. 10.3389/fcimb.2014.00129 · DOI
  67. 10.1073/pnas.111128198 · DOI
  68. 10.1073/pnas.89.19.9257 · DOI
  69. 10.1093/nar/gkv550 · DOI
  70. 10.1002/pmic.201500108 · DOI
  71. 10.1093/bfgp/ell018 · DOI
  72. 10.1091/mbc.E15-12-0844 · DOI
  73. 10.7554/eLife.06547 · DOI

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Metadata

Authors
Varnaitė, R., & MacNeill, S. A.
Affiliations
School of Biology University of St Andrews North Haugh St Andrews Scotland, UK
Venue
PROTEOMICS
Publisher
Wiley
Published
2016-07-27
Volume
16
Issue
19
Pages
2503-2518
ISSN
  • 1615-9853
  • 1615-9861
DOI
10.1002/pmic.201600123
PMC
PMC5053326
Cited by
173
References
6
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)
Funders
  • Medical Research Council (MR/M015483/1)
  • Scottish Funding Council

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