The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Evaluation of the impact of some experimental procedures on different phosphopeptide enrichment techniques
Abstract
The complete characterization of phosphorylated proteins requires an efficient procedure for the enrichment of phosphopeptides from amongst a complicated peptide mixture. The sensitivity of the traditional immobilized metal affinity chromatography (IMAC) approach is severely affected by various buffers, detergents and other reagents normally utilized in biochemical and cell biological procedures, and thus pre‐purification steps such as reversed‐phase chromatography is required prior to phosphopeptide enrichment. Here we evaluate the use of different ‘non‐phosphopeptide‐excluding compounds’ in the loading buffer for titanium dioxide (TiO 2 ) chromatography and show that TiO 2 is more robust and tolerant towards many reagents, including salts, detergents and other low molecular mass molecules, than conventional IMAC. In addition, we show that the inclusion of various detergents can enhance the efficiency of this enrichment method, as phosphopeptides that otherwise adhere to plastic surfaces can be efficiently solubilized and subsequently purified. The TiO 2 chromatography technique is also compared to zirconium dioxide chromatography for phosphopeptide enrichment. Copyright © 2007 John Wiley & Sons, Ltd.
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Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae
Ficarro, S. B., McCleland, M. L., Stukenberg, P. T., Burke, D. J., Ross, M. M., Shabanowitz, J., Hunt, D. F., & White, F. M. (2002) · Nature Biotechnology · doi:10.1038/nbt0302-301 · Cited by 1,636
Larsen, M. R., Thingholm, T. E., Jensen, O. N., Roepstorff, P., & Jørgensen, T. J. D. (2005) · Molecular & Cellular Proteomics · doi:10.1074/mcp.t500007-mcp200 · Cited by 1,476
Self-citationPinkse, M. W. H., Uitto, P. M., Hilhorst, M. J., Ooms, B., & Heck, A. J. R. (2004) · Analytical Chemistry · doi:10.1021/ac0498617 · Cited by 891
Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteome
Oda, Y., Nagasu, T., & Chait, B. T. (2001) · Nature Biotechnology · doi:10.1038/86783 · Cited by 777
Quantitative Phosphoproteomics Applied to the Yeast Pheromone Signaling Pathway
Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Færgeman, N. J., Mann, M., & Jensen, O. N. (2005) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m400219-mcp200 · Cited by 763
A systematic approach to the analysis of protein phosphorylation
Zhou, H., Watts, J. D., & Aebersold, R. (2001) · Nature Biotechnology · doi:10.1038/86777 · Cited by 714
Highly selective enrichment of phosphorylated peptides using titanium dioxide
Thingholm, T. E., Jørgensen, T. J. D., Jensen, O. N., & Larsen, M. R. (2006) · Nature Protocols · doi:10.1038/nprot.2006.185 · Cited by 618
Self-citationNühse, T. S., Stensballe, A., Jensen, O. N., & Peck, S. C. (2003) · Molecular & Cellular Proteomics · doi:10.1074/mcp.t300006-mcp200 · Cited by 545
Kweon, H. K., & Håkansson, K. (2006) · Analytical Chemistry · doi:10.1021/ac0522355 · Cited by 489
18 O Labeling: a tool for proteomics
Stewart, I. I., Thomson, T., & Figeys, D. (2001) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.525 · Cited by 327
Cited by
Proteomics by Mass Spectrometry: Approaches, Advances, and Applications
Yates, J. R., Ruse, C. I., & Nakorchevsky, A. (2009) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-061008-124934 · Cited by 1,078
Analytical strategies for phosphoproteomics
Thingholm, T. E., Jensen, O. N., & Larsen, M. R. (2009) · PROTEOMICS · doi:10.1002/pmic.200800454 · Cited by 477
Self-citationThingholm, T. E., Jensen, O. N., Robinson, P. J., & Larsen, M. R. (2008) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m700362-mcp200 · Cited by 425
Self-citationZhao, Y., & Jensen, O. N. (2009) · PROTEOMICS · doi:10.1002/pmic.200900398 · Cited by 384
Le Bihan, M.-C., Bigot, A., Jensen, S. S., Dennis, J. L., Rogowska-Wrzesinska, A., Lainé, J., Gache, V., Furling, D., Jensen, O. N., Voit, T., et al. (2012) · Journal of Proteomics · doi:10.1016/j.jprot.2012.09.008 · Cited by 153
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Metadata
- Authors
- Jensen, S. S., & Larsen, M. R.
- Venue
- Rapid Communications in Mass Spectrometry
- Publisher
- Wiley
- Published
- 2007-10-25
- Volume
- 21
- Issue
- 22
- Pages
- 3635-3645
- ISSN
- 0951-4198 (ISSN-L)
- 1097-0231
- OpenAlex
- W1983836332
- Cited by
- 274
- References
- 19
- FWCI
- 10.75
- Type
- journal article
- Language
- en
- Open access
- closed
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