A Spontaneous 3D Bone‐On‐a‐Chip for Bone Metastasis Study of Breast Cancer Cells
Abstract
Bone metastasis occurs at ≈70% frequency in metastatic breast cancer. The mechanisms used by tumors to hijack the skeleton, promote bone metastases, and confer therapeutic resistance are poorly understood. This has led to the development of various bone models to investigate the interactions between cancer cells and host bone marrow cells and related physiological changes. However, it is challenging to perform bone studies due to the difficulty in periodic sampling. Herein, a bone‐on‐a‐chip (BC) is reported for spontaneous growth of a 3D, mineralized, collagenous bone tissue. Mature osteoblastic tissue of up to 85 µm thickness containing heavily mineralized collagen fibers naturally formed in 720 h without the aid of differentiation agents. Moreover, co‐culture of metastatic breast cancer cells is examined with osteoblastic tissues. The new bone‐on‐a‐chip design not only increases experimental throughput by miniaturization, but also maximizes the chances of cancer cell interaction with bone matrix of a concentrated surface area and facilitates easy, frequent observation. As a result, unique hallmarks of breast cancer bone colonization, previously confirmed only in vivo, are observed. The spontaneous 3D BC keeps the promise as a physiologically relevant model for the in vitro study of breast cancer bone metastasis.
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A 3D bone chip with mineralized collagen bone tissue growing naturally has been reported.
“There is a report of a 3D bone chip with mineralized collagen bone tissue growing naturally (Fig. 6a)213.”
From Tumor-on-a-chip: from bioinspired design to biomedical application (2021) · cites this paper as [213]
The 3D bone chip model provides a new in vitro model for studying bone metastases from breast tumors.
“This model provides a new in vitro model for studying bone metastases from breast tumors213.”
From Tumor-on-a-chip: from bioinspired design to biomedical application (2021) · cites this paper as [213]
A bone metastasis model of breast tumor cells was established based on the 3D bone chip.
“Based on this chip, a bone metastasis model of breast tumor cells was established.”
From Tumor-on-a-chip: from bioinspired design to biomedical application (2021) · cites this paper as [213]
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- Authors
- Hao, S., Ha, L., Cheng, G., Wan, Y., Xia, Y., Sosnoski, D. M., Mastro, A. M., & Zheng, S.
- Affiliations
- Pennsylvania State University (US)
- Department of Biomedical Engineering Micro & Nano Integrated Biosystem (MINIBio) Laboratory The Pennsylvania State University University Park PA 16802 USA
- Venue
- Small
- Publisher
- Wiley
- Published
- 2018-02-05
- Volume
- 14
- Issue
- 12
- Pages
- e1702787-e1702787
- ISSN
- 1613-6810 (ISSN-L)
- 1613-6829
- OpenAlex
- W2792221286
- Cited by
- 195
- References
- 33
- Citations by year
- 2026: 21 · 2025: 18 · 2024: 25 · 2023: 20 · 2022: 32 · 2021: 26 · 2020: 31 · 2019: 16 · 2018: 6
- FWCI
- 7.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Small (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Cancer Society (RSG‐13‐009‐01‐CDD)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- breast cancer bone metastasis, osteoblasts, collagen fibers, bone mineralization, bone colonization, in vitro bone model
- MeSH terms
- Bone Neoplasms, Breast Neoplasms, Female, Humans, Neoplasm Metastasis, Coculture Techniques, Microfluidics, Cell Line, Tumor
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