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Cited by 311

The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.

miR-200 Regulates PDGF-D-Mediated Epithelial–Mesenchymal Transition, Adhesion, and Invasion of Prostate Cancer Cells

Open access
Kong, Dejuan, Li, Yiwei, Wang, Zhiwei, Banerjee, Sanjeev, Ahmad, Aamir, Kim, Hyeong-Reh Choi, Sarkar, Fazlul H.·2009·Stem Cells
DOI 10.1002/stem.101
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Abstract

MicroRNAs have been implicated in tumor progression. Recent studies have shown that the miR-200 family regulates epithelial–mesenchymal transition (EMT) by targeting zinc-finger E-box binding homeobox 1 (ZEB1) and ZEB2. Emerging evidence from our laboratory and others suggests that the processes of EMT can be triggered by various growth factors, such as transforming growth factor β and platelet-derived growth factor-D (PDGF-D). Moreover, we recently reported that overexpression of PDGF-D in prostate cancer cells (PC3 PDGF-D cells) leads to the acquisition of the EMT phenotype, and this model offers an opportunity for investigating the molecular interplay between PDGF-D signaling and EMT. Here, we report, for the first time, significant downregulation of the miR-200 family in PC3 PDGF-D cells as well as in PC3 cells exposed to purified active PDGF-D protein, resulting in the upregulation of ZEB1, ZEB2, and Snail2 expression. Interestingly, re-expression of miR-200b in PC3 PDGF-D cells led to reversal of the EMT phenotype, which was associated with the downregulation of ZEB1, ZEB2, and Snail2 expression, and these results were consistent with greater expression levels of epithelial markers. Moreover, transfection of PC3 PDGF-D cells with miR-200b inhibited cell migration and invasion, with concomitant repression of cell adhesion to the culture surface and cell detachment. From these results, we conclude that PDGF-D-induced acquisition of the EMT phenotype in PC3 cells is, in part, a result of repression of miR-200 and that any novel strategy by which miR-200 could be upregulated would become a promising approach for the treatment of invasive prostate cancer. Disclosure of potential conflicts of interest is found at the end of this article.

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Metadata

Authors
Kong, D., Li, Y., Wang, Z., Banerjee, S., Ahmad, A., Kim, H.-R. C., & Sarkar, F. H.
Venue
Stem Cells
Publisher
Oxford University Press (OUP)
Published
2009-04-23
Volume
27
Issue
8
Pages
1712-1721
ISSN
  • 1066-5099 (ISSN-L)
  • 1549-4918
DOI
10.1002/stem.101
OpenAlex
W2033681249
Cited by
311
References
38
FWCI
10.68
Type
journal article
Language
en
Open access
bronze

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