Cloning and analysis of CoEXG1, a secreted 1,3‐β‐glucanase of the yeast biocontrol agent Candida oleophila
Abstract
Lytic enzymes may have a role in the biological control of fungi. The yeast biocontrol agent, Candida oleophila, is an excellent subject to research this matter. In the present study, CoEXG1, which encodes for a secreted 1,3‐β‐glucanase, is the first gene to be cloned from C. oleophila. It was isolated from a partial genomic library and analysed. Its open reading frame and putative promoter were expressed in baker's yeast, Saccharomyces cerevisiae. The reading frame, expressed under the inducible GAL1 promoter, caused an increased secretion of β‐glucanase, and the putative promoter region activated the lacZ reporter gene, to which it was fused. Sequencing analysis revealed that CoEXG1 carries the signature pattern of the 5 glycohydrolases family and has a putative secretion leader, as well as a high degree of identity to yeast 1,3‐β‐glucanases. The GenBank Accession No. of CoEXG1 is AF393806. Copyright © 2002 John Wiley & Sons, Ltd.
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A 1,3-β-glucanase (CoEXG1) from Candida oleophila was the first gene cloned in this organism.
“A 1,3-β-glucanase (CoEXG1) from Candida oleophila was the first gene cloned in this organism (Segal et al. 2002).”
From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Segal et al. 2002
Work on C. oleophila and other Candida species identified hydrolytic enzymes such as proteases, chitinases and glucanases, as well as volatile compounds, that have been implicated in antifungal activity.
“the work on C. oleophila and other Candida species identified hydrolytic enzymes such as proteases, chitinases and glucanases, as well as volatile compounds, that have been implicated in antifungal activity (Bar-Shimon et al. 2004; Huang et al. 2011; Segal et al. 2002)”
From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Segal et al. 2002
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(2002) · Yeast · doi:10.1002/yea.940
- Authors
- Segal, E., Yehuda, H., Droby, S., Wisniewski, M., & Goldway, M.
- Affiliations
- Migal - Galilee Technology Center (IL)
- Agricultural Research Organization (IL)
- Appalachian Fruit Research Laboratory (US)
- Corresponding author
- Goldway, Martin
- Venue
- Yeast
- Publisher
- Wiley
- Published
- 2002-08-29
- Volume
- 19
- Issue
- 13
- Pages
- 1171-1182
- ISSN
- 0749-503X (ISSN-L)
- 1097-0061
- OpenAlex
- W2161461054
- Cited by
- 18
- References
- 45
- Citations by year
- 2024: 1 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 1 · 2014: 1 · 2012: 1
- FWCI
- 0.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Yeast (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- Candida oleophila, β-1,3-glucanase, secreted enzymes, lytic enzymes, biological control of fungi, gene cloning, promoter analysis, secretion signal, Saccharomyces cerevisiae expression, lacZ reporter
- MeSH terms
- Amino Acid Sequence, Base Sequence, beta-Glucosidase, Candida, Cloning, Molecular, Fungicides, Industrial, Molecular Sequence Data, Pest Control, Biological, Promoter Regions, Genetic, Glucan 1,3-beta-Glucosidase
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