The DOI matches its Crossref record.
The L-Arginine: Nitric Oxide Pathway Is the Major Source of Plasma Nitrite in Fasted Humans
Abstract
15N guanidino-labelled L-arginine was infused into fasted human volunteers giving, at equilibrium, a stable 1:10 ratio of 15N to 14N arginine in the plasma. Separate GC-MS assays were used to compare the degree of enrichment of plasma arginine, nitrite and nitrate and thus define the quantitative relationship between the L-arginine:nitric oxide (NO) pathway and the formation of these oxides of nitrogen. 15N nitrite enrichments rose to 8.3% (SD 0.5), five hours after the start of the infusion. In contrast, 15N nitrate enrichments apparently rose to only 1.6% (SD 0.4) at this time. This discrepancy could be explained by our finding that the commonly used Tesch GC-MS nitrate assay is subject to considerable interference from non-nitrate sources in plasma. Taking this into account, nitrate enrichments were similar to those observed for plasma nitrite. These results therefore indicate that the measurement of these compounds in plasma is a valid indicator of NO generation in fasted humans.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“It should be remembered that nitrate is also produced endogenously.”
From Vascular effects of dietary nitrate (as found in green leafy vegetables and beetroot) via the nitrate-nitrite-nitric oxide pathway (2013) · cites this paper as [52]
Citations
Cites
Still being checked.
Cited by
Sorted by citations
Methods to detect nitric oxide and its metabolites in biological samples
Bryan, N. S., & Grisham, M. B. (2007) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2007.04.026 · Cited by 1,005
Tsikas, D. (2006) · Journal of Chromatography B · doi:10.1016/j.jchromb.2006.07.054 · Cited by 897
Nitric oxide metabolism and breakdown
Kelm, M. (1999) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(99)00020-1 · Cited by 673
Astaxanthin, a Carotenoid with Potential in Human Health and Nutrition
Hussein, G. M. E., Sankawa, U., Goto, H., Matsumoto, K., & Watanabe, H. (2005) · Journal of Natural Products · doi:10.1021/np050354+ · Cited by 653
Plasma nitrite reflects constitutive nitric oxide synthase activity in mammals
Kleinbongard, P., Dejam, A., Lauer, T., Rassaf, T., Schindler, A., Picker, O., Scheeren, T., Gödecke, A., Schrader, J., Schulz, R., et al. (2003) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(03)00406-4 · Cited by 586
Metadata
- Authors
- Rhodes, P. M., Leone, A. M., Francis, P. L., Struthers, A. D., & Moncada, S.
- Affiliations
- Ninewells Hospital (GB)
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1995-04
- Volume
- 209
- Issue
- 2
- Pages
- 590-596
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2049563995
- Cited by
- 254
- References
- 0
- Citations by year
- 2025: 2 · 2024: 3 · 2022: 4 · 2021: 3 · 2020: 5 · 2019: 5 · 2018: 8 · 2017: 6 · 2016: 3 · 2015: 9 · 2014: 12 · 2013: 9 · 2012: 9
- FWCI
- 3.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Discovery Research Portal (University of Dundee) (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- L-arginine, nitric oxide, nitrite, nitrate, arginine infusion, fasting state, plasma nitrite, nitrogen oxides
- MeSH terms
- Adult, Arginine, Fasting, Humans, Male, Gas Chromatography-Mass Spectrometry, Nitric Oxide, Nitrites
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
