Cited by 83Neuroinflammation and Neurodegeneration MechanismsAlzheimer's disease research and treatmentsNeuropeptides and Animal Physiology

α-MSH Peptides Inhibit Production of Nitric Oxide and Tumor Necrosis Factor-α by Microglial Cells Activated with β-Amyloid and Interferon γ

Galimberti, Daniela, Baron, Pierluigi, Meda, Lucia, Prat, Elisabetta, Scarpini, Elio, Delgado, René, Catania, Anna, Lipton, James M. +1 more·1999·Biochemical and Biophysical Research Communications

Abstract

Alpha-melanocyte stimulating hormone (alpha-MSH) is an ancient tridecapeptide with potent inhibitory activity in all major forms of inflammation. The anti-inflammatory message sequence of alpha-MSH resides in the COOH-terminal tripeptide alpha-MSH[11-13]. We tested the influence of alpha-MSH[1-13] and of alpha-MSH[11-13] in a cultured murine microglia cell line known to produce nitric oxide (NO(-)(2)) and tumor necrosis factor (TNFalpha) when stimulated with beta-amyloid protein (Abeta). Melanocortin peptides significantly inhibited release of both NO(-)(2) and TNFalpha into cell-free supernatants from microglia stimulated with Abeta[1-42] or Abeta[25-35] peptides and interferon gamma (IFNgamma). Northern blot analysis demonstrated that alpha-MSH[1-13] and alpha-MSH[11-13] inhibited accumulation of inducible nitric oxide synthase (iNOS) and TNFalpha mRNA was triggered by Abeta stimulation. Abeta/microglial interaction is believed to promote the progression of inflammatory and neurodegenerative changes in senile plaques in Alzheimer's disease. Our data indicate that alpha-MSH peptides might be used to modulate the local response of the brain to Abeta deposition in this neurodegenerative disease.

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Crossref · 10.1006/bbrc.1999.1276

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Authors
Galimberti, D., Baron, P., Meda, L., Prat, E., Scarpini, E., Delgado, R., Catania, A., Lipton, J. M., & Scarlato, G.
Affiliations
  • University of Milan (IT)
  • The University of Texas Southwestern Medical Center (US)
Corresponding author
Baron, Pierluigi
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
1999-09
Volume
263
Issue
1
Pages
251-256
ISSN
0006-291X (ISSN-L)
OpenAlex
W2435170652
Cited by
83
References
34
Citations by year
2024: 2 · 2023: 2 · 2021: 4 · 2020: 2 · 2019: 3 · 2018: 5 · 2017: 4 · 2016: 1 · 2015: 2 · 2014: 2 · 2013: 5 · 2012: 2
FWCI
3.02
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
Keywords
alpha-melanocyte-stimulating hormone, microglia, nitric oxide, TNF-α, amyloid-beta, interferon-gamma, inducible nitric oxide synthase, anti-inflammatory peptides, Alzheimer's disease, neuroinflammation, amyloid plaques
MeSH terms
alpha-MSH, Animals, Cell Line, Interferon-gamma, Melanocyte-Stimulating Hormones, Nitric Oxide, Peptide Fragments, Recombinant Proteins, RNA, Messenger, Tumor Necrosis Factor-alpha, Amyloid beta-Peptides, Microglia, Inflammation Mediators, Nitric Oxide Synthase, Mice, Nitric Oxide Synthase Type II

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