Selective Inhibition by α-Tocopherol of Vascular Cell Adhesion Molecule-1 Expression in Human Vascular Endothelial Cells

Zapolska-Downar, D.; Zapolski-Downar, A.; Markiewski, M.; Ciechanowicz, A.; Kaczmarczyk, M.; Naruszewicz, M.

doi:10.1006/bbrc.2000.3197

Cited by 54Cell Adhesion Molecules ResearchAntioxidant Activity and Oxidative StressAtherosclerosis and Cardiovascular Diseases

Selective Inhibition by α-Tocopherol of Vascular Cell Adhesion Molecule-1 Expression in Human Vascular Endothelial Cells

Zapolska-Downar, D., Zapolski-Downar, A., Markiewski, M., Ciechanowicz, A., Kaczmarczyk, M., Naruszewicz, M.·2000·Biochemical and Biophysical Research Communications

DOI 10.1006/bbrc.2000.3197

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Abstract

An early event in atherogenesis is the adhesion of monocytes to endothelium via adhesion molecules such as VCAM-1 and intracellular adhesion molecule-1 (ICAM-1). It has been suggested that VCAM-1 plays a very important role in recruitment of monocytes in atherosclerosis. Several studies suggest that vitamin E has antiatherosclerotic properties. However, the mechanism of its antiatherogenic effect awaits elucidation. The aim of our study was to evaluate whether alpha-tocopherol can influence expression of endothelial cell adhesion molecules and endothelial adhesiveness. The study was performed on cultured human umbilical vein endothelial cells (HUVEC). HUVEC were pretreated with alpha-tocopherol (50 micromol/l) in different times before stimulation with TNFalpha (100 U/ml) or IL-1beta (100 U/ml). Protein expression of VCAM-1 and ICAM-1 was measured by flow cytometry. mRNA expression of VCAM-1 was measured by reverse transcription polymerase chain reaction (RT-PCR). alpha-Tocopherol time dependently reduced agonist-induced VCAM-1 in both surface protein (about 40%, 48 h) and mRNA (about 35%, 48 h) expression in HUVEC but not ICAM-1 surface protein expression. Inhibitory effect of alpha-tocopherol was dependent on culture condition of HUVEC. Decreased VCAM-1 expression was associated with reduction (about 40%) of adherence between cytokine-stimulated HUVEC and peripheral blood mononuclear leukocytes (PBMC). Our results suggest that the antiatherogenic effect of alpha-tocopherol may in part be due to a downregulation of VCAM-1 expression.

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“Vitamin E has been reported to decrease monocyte recruitment as a result of suppressed formation of foam cells and reduce expression of scavenger receptors (SRs) on macrophages (Zapolska-Downar et al. 2000).”
From Reactive Oxygen Species, Toxicity, Oxidative Stress, and Antioxidants: Chronic Diseases and Aging (2023) · cites this paper as Zapolska-Downar et al. 2000

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Authors: Zapolska-Downar, D., Zapolski-Downar, A., Markiewski, M., Ciechanowicz, A., Kaczmarczyk, M., & Naruszewicz, M.
Affiliations: Pomeranian Medical University (PL)
ORCID: Markiewski, M.
Ciechanowicz, A.
Kaczmarczyk, M.
Venue: Biochemical and Biophysical Research Communications
Publisher: Elsevier BV
Published: 2000-08
Volume: 274
Issue: 3
Pages: 609-615
ISSN: 0006-291X (ISSN-L)
DOI: 10.1006/bbrc.2000.3197
OpenAlex: W2057459722
Cited by: 54
References: 49
Citations by year: 2023: 2 · 2022: 1 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 2 · 2014: 1 · 2012: 3
FWCI: 1.97
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Biochemical and Biophysical Research Communications (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
Keywords: alpha-tocopherol, vitamin E, VCAM-1, ICAM-1, endothelial cells, atherosclerosis, monocyte adhesion, TNF-α, IL-1β, gene expression regulation, antiatherogenic effects, leukocyte adhesion
MeSH terms: Arteriosclerosis, Cell Adhesion, Cell Line, Endothelium, Vascular, Humans, Monocytes, RNA, Messenger, Vitamin E, Intercellular Adhesion Molecule-1, Vascular Cell Adhesion Molecule-1

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Selective Inhibition by α-Tocopherol of Vascular Cell Adhesion Molecule-1 Expression in Human Vascular Endothelial Cells

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