Cited by 27Crustacean biology and ecologyEnvironmental Toxicology and EcotoxicologyAnimal testing and alternatives

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Optimization of Culture Conditions of Porcellio dilatatus (Crustacea: Isopoda) for Laboratory Test Development

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Caseiro, Isabel, Santos, S., Sousa, J.P., Nogueira, A.J.A., Soares, A.M.V.M.·2000·Ecotoxicology and Environmental Safety

Abstract

This paper describes the experimental results for optimizing isopod culture conditions for terrestrial ecotoxicity testing. The influence of animal density and food quality on growth and reproduction of Porcellio dilatatus was investigated. Results indicate that density influences isopod performance in a significant way, with low-density cultures having a higher growth rate and better reproductive output than medium- or high-density cultures. Alder leaves, as a soft nitrogen-rich species, were found to be the best-quality diet; when compared with two other food mixtures, alder leaves induced the best results, particularly in terms of breeding success. Guidance regarding culture conditions of isopod species, especially in terms of culture density and diet, is given.

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References 32 · Cited by 27

Research works printed but not linked: 27

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Metadata

Authors
Caseiro, I., Santos, S., Sousa, J. P., Nogueira, A. J. A., & Soares, A. M. V. M.
Affiliations
  • University of Coimbra (PT)
  • Universidade Politécnica de Bragança (PT)
  • University of Aveiro (PT)
Corresponding author
Sousa, J.P.
Venue
Ecotoxicology and Environmental Safety
Publisher
Elsevier BV
Published
2000-11
Volume
47
Issue
3
Pages
285-291
ISSN
0147-6513 (ISSN-L)
OpenAlex
W2034826595
Cited by
27
References
32
Citations by year
2026: 1 · 2025: 2 · 2024: 1 · 2022: 1 · 2019: 1 · 2018: 3 · 2017: 1 · 2015: 2 · 2014: 1 · 2013: 1
FWCI
0.00
Type
journal article
Language
en
Open access
Locations
  • Ecotoxicology and Environmental Safety (publishedVersion)
  • PubMed (publishedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion)
Topics
  • Physical Sciences › Environmental Science › Ecology › Crustacean biology and ecology
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
  • Health Sciences › Veterinary › Small Animals › Animal testing and alternatives
Keywords
Porcellio dilatatus, terrestrial isopods, animal density, food quality, alder leaves, growth rate, reproductive output
MeSH terms
Animals, Crustacea, Culture Media, Diet, Population Density, Reproduction, Guidelines as Topic, Toxicity Tests

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