Regulation of Brain-Derived Neurotrophic Factor Gene Expression after Transient Middle Cerebral Artery Occlusion with and without Brain Damage
Abstract
Levels of mRNA for c-fos, nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), TrkB, and TrkC were studied using in situ hybridization in the rat brain at different reperfusion times after unilateral middle cerebral artery occlusion (MCAO). Short-term (15 min) MCAO, which does not cause neuronal death, induced elevated BDNF mRNA expression confined to ipsilateral frontal and cingulate cortices outside the ischemic area. With a longer duration of MCAO (2 h), which leads to cortical infarction, the increase was more marked and elevated BDNF mRNA levels were also detected bilaterally in dentate granule cells and CA1 and CA3 pyramidal neurons. Maximum expression was found after 2 h of reperfusion. At 24 h BDNF mRNA expression had returned to control values. In the ischemic core of the parietal cortex only scattered neurons were expressing high levels of BDNF mRNA after 15 min and 2 h of MCAO. Analysis of different BDNF transcripts showed that MCAO induced a marked increase of exon III mRNA but only small increases of exon I and II mRNAs in cortex and hippocampus. In contrast to BDNF mRNA, elevated expression of c-fos mRNA was observed in the entire ipsilateral cerebral cortex, including the ischemic core, after both 15 min and 2 h of MCAO. Two hours of MCAO also induced transient, bilateral increases of NGF and TrkB mRNA levels and a decrease of NT-3 mRNA expression, confined to dentate granule cells. The upregulation of BDNF mRNA expression…
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The upregulation of Bdnf transcription in the ischemic cortex, including the damaged area and periinfarction zone, was shown in our study to correspond with other evidence.
“The upregulation of Bdnf transcription in the ischemic cortex, including the damaged area and periinfarction zone, was shown in our study to correspond with other evidence (Kokaia et al. 1995; Ferrer et al. 2001; Pera et al. 2005; Kim et al. 2005).”
From Semax and Pro-Gly-Pro activate the transcription of neurotrophins and their receptor genes after cerebral ischemia (2010) · cites this paper as Kokaia et al. 1995
In our study, we did not observe any increase in TrkB transcription at the earliest time point (3 h).
“In our study, we did not observe any increase in TrkB transcription at the earliest time point (3 h) reported in the other studies (Majda et al. 2001; Kokaia et al. 1995).”
From Semax and Pro-Gly-Pro activate the transcription of neurotrophins and their receptor genes after cerebral ischemia (2010) · cites this paper as Kokaia et al. 1995
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- Authors
- Kokaia, Z., Zhao, Q., Kokaia, M., Elmér, E., Metsis, M., Smith, M.-L., Siesjö, B. K., & Lindvall, O.
- Affiliations
- Lund University (SE)
- Venue
- Experimental Neurology
- Publisher
- Elsevier BV
- Published
- 1995-11
- Volume
- 136
- Issue
- 1
- Pages
- 73-88
- ISSN
- 0014-4886 (ISSN-L)
- OpenAlex
- W2011903589
- Cited by
- 252
- References
- 0
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 5 · 2021: 5 · 2020: 6 · 2019: 6 · 2018: 11 · 2017: 6 · 2016: 8 · 2015: 6 · 2014: 10 · 2013: 6 · 2012: 12
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental Neurology (publishedVersion)
- PubMed (publishedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
- Keywords
- brain-derived neurotrophic factor, gene expression, middle cerebral artery occlusion, cerebral ischemia, reperfusion, c-fos, nerve growth factor, neurotrophin-3, TrkB, TrkC, dentate gyrus granule cells, hippocampus, cortical infarction, glutamate, cortical spreading depression, neuroprotection, epileptogenesis, synaptic efficacy
- MeSH terms
- Animals, Autoradiography, Brain Injuries, Cerebral Arteries, Brain Ischemia, Hippocampus, Male, Nerve Tissue Proteins, RNA, Messenger, Time Factors, Gene Expression, Rats, Wistar, In Situ Hybridization, Brain-Derived Neurotrophic Factor, Rats
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