Cited by 398Nuclear Structure and FunctionProtein Structure and DynamicsGenomics and Chromatin Dynamics

Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-α11Edited by K. Nagai

Fontes, Marcos R.M., Teh, Trazel, Kobe, Bostjan·2000·Journal of Molecular Biology

Abstract

Importin-alpha is the nuclear import receptor that recognizes cargo proteins which contain classical monopartite and bipartite nuclear localization sequences (NLSs), and facilitates their transport into the nucleus. To determine the structural basis of the recognition of the two classes of NLSs by mammalian importin-alpha, we co-crystallized an N-terminally truncated mouse receptor protein with peptides corresponding to the monopartite NLS from the simian virus 40 (SV40) large T-antigen, and the bipartite NLS from nucleoplasmin. We show that the monopartite SV40 large T-antigen NLS binds to two binding sites on the receptor, similar to what was observed in yeast importin-alpha. The nucleoplasmin NLS-importin-alpha complex shows, for the first time, the mode of binding of bipartite NLSs to the receptor. The two basic clusters in the NLS occupy the two binding sites used by the monopartite NLS, while the sequence linking the two basic clusters is poorly ordered, consistent with its tolerance to mutations. The structures explain the structural basis for binding of diverse NLSs to the sole receptor protein.

Integrity signals

Registry

Crossref · 10.1006/jmbi.2000.3642

Cites

45 of 45 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Fontes, M. R. M., Teh, T., & Kobe, B.
Affiliations
St Vincents Institute of Medical Research (AU)
Venue
Journal of Molecular Biology
Publisher
Elsevier BV
Published
2000-04
Volume
297
Issue
5
Pages
1183-1194
ISSN
0022-2836 (ISSN-L)
OpenAlex
W2049132410
Cited by
398
References
45
Citations by year
2026: 15 · 2025: 12 · 2024: 7 · 2023: 14 · 2022: 15 · 2021: 16 · 2020: 11 · 2019: 8 · 2018: 12 · 2017: 19 · 2016: 19 · 2015: 25 · 2014: 10 · 2013: 20 · 2012: 13
Type
book review
Language
en
Open access
closed
Locations
  • Journal of Molecular Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
nuclear localization signal, bipartite NLS, nuclear import, SV40 large T antigen, nucleoplasmin, protein-peptide complex structure, receptor binding sites, nuclear transport receptors
MeSH terms
Amino Acid Sequence, Animals, Binding Sites, Models, Molecular, Molecular Sequence Data, Mutation, Nuclear Proteins, Oligopeptides, Peptide Fragments, Phosphoproteins, Protein Binding, Structure-Activity Relationship, Substrate Specificity, Yeasts, Conserved Sequence, Protein Structure, Secondary, Protein Structure, Tertiary, Crystallography, X-Ray, Nuclear Localization Signals, Karyopherins, Mice, Nucleoplasmins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review