Cited by 89Protein Kinase Regulation and GTPase SignalingFungal and yeast genetics researchMetabolism, Diabetes, and Cancer

The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization

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NALASKOWSKI, Marcus M., DESCHERMEIER, Christina, FANICK, Werner, MAYR, Georg W.·2002·Biochemical Journal

Abstract

The function of the transcription regulator ArgRIII in the expression of several genes involved in the metabolism of arginine in yeast has been well studied. It was previously reported that it is also an inositol phosphate multikinase and an important factor of the mRNA export pathway [reviewed by Shears (2000) Bioessays 22, 786–789]. In the present study we report the cloning of a full-length 1248-bp cDNA encoding a human inositol phosphate multikinase (IPMK). This protein has a calculated molecular mass of 47.219kDa. Functionally important motifs [inositol phosphate-binding site, ATP-binding site, catalytically important SSLL (Ser-Ser-Leu-Leu) domain] are conserved between the human IPMK and yeast ArgRIII. Bacterially expressed protein demonstrated an inositol phosphate multikinase activity similar to that of yeast ArgRIII. Ins(1,4,5)P3 is phosphorylated at positions 3 and 6 up to Ins(1,3,4,5,6)P5. The human IPMK fused with a fluorescent protein tag is localized predominantly in the nucleus when transiently expressed in mammalian cells. A basic cluster in the protein's C-terminus is positively involved in nuclear targeting. These findings are consistent with the concept of a nuclear inositol phosphate signalling and phosphorylation pathway in mammalian cells.

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Crossref · 10.1042/bj20020327

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Authors
NALASKOWSKI, M. M., DESCHERMEIER, C., FANICK, W., & MAYR, G. W.
Affiliations
University Medical Center Hamburg-Eppendorf (DE)
Venue
Biochemical Journal
Published
2002-09-01
Volume
366
Issue
2
Pages
549-556
ISSN
  • 0264-6021 (ISSN-L)
  • 1470-8728
OpenAlex
W1994071091
Cited by
89
References
29
Citations by year
2026: 2 · 2025: 1 · 2024: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 5 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 2 · 2012: 6
FWCI
2.21
Type
journal article
Language
en
Open access
Locations
  • Biochemical Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Keywords
cDNA cloning, inositol trisphosphate, inositol pentakisphosphate, nuclear localization, mRNA export
MeSH terms
Amino Acid Sequence, Animals, Arginine, Base Sequence, Binding Sites, Cell Line, Cell Nucleus, Cloning, Molecular, Fungal Proteins, Humans, Inositol Phosphates, Kidney, Molecular Sequence Data, Recombinant Proteins, Transfection, Polymerase Chain Reaction, Mutagenesis, Site-Directed, Phosphotransferases (Alcohol Group Acceptor), DNA Primers, Saccharomyces cerevisiae Proteins, Rats

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