Tools and Techniques for Chloroplast Transformation of Chlamydomonas

Oxygenic Photosynthesis: The Light Reactions

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Chloroplast Transformation in Chlamydomonas with High Velocity Microprojectiles

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PLASTID TRANSFORMATION IN HIGHER PLANTS

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Transgenic expression of aminoglycoside adenine transferase in the chloroplast: a selectable marker for site-directed transformation of chlamydomonas

Goldschmidt‐Clermont, M. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.15.4083 · Cited by 473 · see citing works

The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas

Rochaix, J.-D., Goldschmidt‐Clermont, M., & Merchant, S. (1998) · Advances in photosynthesis and respiration · doi:10.1007/0-306-48204-5 · Cited by 450 · see citing works

COMMERCIAL DEVELOPMENTS IN MICROALGAL BIOTECHNOLOGY

Apt, K. E., & Behrens, P. W. (1999) · Journal of Phycology · doi:10.1046/j.1529-8817.1999.3520215.x · Cited by 393 · see citing works

Expression and assembly of a fully active antibody in algae

Mayfield, S. P., Franklin, S. E., & Lerner, R. A. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0237108100 · Cited by 345 · see citing works

Evidence for two active branches for electron transfer in photosystem I

Guergova-Kuras, M., Boudreaux, B., Joliot, A., Joliot, P. A., & Redding, K. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.081078898 · Cited by 335 · see citing works

The biolistic process

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CHLAMYDOMONAS REINHARDTII AS THE PHOTOSYNTHETIC YEAST

Rochaix, J.-D. (1995) · Annual Review of Genetics · doi:10.1146/annurev.ge.29.120195.001233 · Cited by 260 · see citing works

Searching limiting steps in the expression of chloroplast‐encoded proteins: relations between gene copy number, transcription, transcript abundance and translation rate in the chloroplast of Chlamydomonas reinhardtii

Eberhard, S., Drapier, D., & Wollman, F.-A. (2002) · The Plant Journal · doi:10.1046/j.1365-313x.2002.01340.x · Cited by 227 · see citing works

Photosynthesis in Algae

Larkum, A. W. D., Raven, J. A., & Douglas, S. E. (2003) · Advances in photosynthesis and respiration · doi:10.1007/978-94-007-1038-2 · Cited by 226 · see citing works

Engineering the chloroplast genome: techniques and capabilities for chloroplast transformation in Chlamydomonas reinhardtii.

Kindle, K. L., Richards, K. L., & Stern, D. B. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.5.1721 · Cited by 211 · see citing works

Development of a GFP reporter gene for Chlamydomonas reinhardtii chloroplast

Franklin, S. E., Ngo, B. X., Efuet, E. T., & Mayfield, S. P. (2002) · The Plant Journal · doi:10.1046/j.1365-313x.2002.01319.x · Cited by 194 · see citing works

Microparticle Bombardment as a Tool in Plant Science and Agricultural Biotechnology

Taylor, N. J., & Fauquet, C. (2002) · DNA and Cell Biology · doi:10.1089/104454902762053891 · Cited by 188 · see citing works

Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast

Sun, M., Qian, K., Su, N., Chang, H., Liu, J., & Shen, G. (2003) · Biotechnology Letters · doi:10.1023/a:1024140114505 · Cited by 185 · see citing works

Selectable marker recycling in the chloroplast

Fischer, N., Stampacchia, O., Redding, K., & Rochaix, J. (1996) · Molecular and General Genetics MGG · doi:10.1007/bf02172529 · Cited by 182 · see citing works

[37] Chloroplast transformation in Chlamydomonas

Boynton, J. E., & Gillham, N. W. (1993) · Methods in Enzymology · doi:10.1016/0076-6879(93)17087-l · Cited by 141 · see citing works

ALGAL TRANSGENICS IN THE GENOMIC ERA1

Walker, T. L., Collet, C., & Purton, S. (2005) · Journal of Phycology · doi:10.1111/j.1529-8817.2005.00133.x · Cited by 137 · see citing works

Prospects for molecular farming in the green alga Chlamydomonas reinhardtii

Franklin, S. E., & Mayfield, S. P. (2004) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2004.01.012 · Cited by 133 · see citing works

Engineering chloroplasts: an alternative site for foreign genes, proteins, reactions and products

Bogorad, L. (2000) · Trends in biotechnology · doi:10.1016/s0167-7799(00)01444-x · Cited by 132 · see citing works

Tools for chloroplast transformation in Chlamydomonas: expression vectors and a new dominant selectable marker

Bateman, J. E., & Purton, S. (2000) · Molecular and General Genetics MGG · doi:10.1007/s004380051184 · Cited by 123 · see citing works

Inducible Trans-activation of Plastid Transgenes: Expression of the R. eutrophaphb Operon in Transplastomic Tobacco

Lössl, A. G., Bohmert, K., Harloff, H., Eibl, C., Mühlbauer, S. K., & Koop, H. (2005) · Plant and Cell Physiology · doi:10.1093/pcp/pci157 · Cited by 123 · see citing works

Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes

Barnes, D., Franklin, S. E., Schultz, J., Henry, R. E., Brown, E. C., Coragliotti, A., & Mayfield, S. P. (2005) · Molecular Genetics and Genomics · doi:10.1007/s00438-005-0055-y · Cited by 105 · see citing works

In vivo analysis of Chlamydomonas chloroplast petD gene expression using stable transformation of beta-glucuronidase translational fusions.

Sakamoto, W., Kindle, K. L., & Stern, D. B. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.2.497 · Cited by 99 · see citing works

Development of a luciferase reporter gene, luxCt, for Chlamydomonas reinhardtii chloroplast

Mayfield, S. P., & Schultz, J. (2004) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01965.x · Cited by 95 · see citing works

Recent developments in the production of human therapeutic proteins in eukaryotic algae

Franklin, S. E., & Mayfield, S. P. (2005) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.5.2.225 · Cited by 92 · see citing works

Inhibition of Chloroplast DNA Recombination and Repair by Dominant Negative Mutants of Escherichia coli RecA

Cerutti, H. D., Johnson, A. M., Boynton, J. E., & Gillham, N. W. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.6.3003 · Cited by 88 · see citing works

CytochromefTranslation in Chlamydomonas Chloroplast Is Autoregulated by its Carboxyl-Terminal Domain[W]

Choquet, Y., Zito, F., Wostrikoff, K., & Wollman, F.-A. (2003) · The Plant Cell · doi:10.1105/tpc.011692 · Cited by 84 · see citing works

A Mutant Strain of Chlamydomonas reinhardtii Lacking the Chloroplast Photosystem II psbI Gene Grows Photoautotrophically

Künstner, P., Guardiola, A., Takahashi, Y., & Rochaix, J. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.16.9651 · Cited by 74 · see citing works

The chloroplast genome.

Sugiura, M. (1995) · PubMed · Cited by 72 · see citing works

Expression of a foreign gene in Chlamydomonas reinhardtii chloroplast

Ishikura, K., Takaoka, Y., Kato, K., Sekine, M., Yoshida, K., & Shinmyō, A. (1999) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(99)80037-1 · Cited by 67 · see citing works

External control of transgene expression in tobacco plastids using the bacterial lac repressor

Mühlbauer, S. K., & Koop, H. (2005) · The Plant Journal · doi:10.1111/j.1365-313x.2005.02495.x · Cited by 66 · see citing works

The Klebsiella pneumoniae nitrogenase Fe protein gene (nifH) functionally substitutes for the chlL gene in Chlamydomonas reinhardtii

Cheng, Q., Day, A., Dowson-Day, M. J., Shen, G., & Dixon, R. A. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.02.064 · Cited by 66 · see citing works

Renilla luciferase as a vital reporter for chloroplast gene expression in Chlamydomonas

Minko, I. G., Holloway, S. P., Nikaido, S. S., Carter, M., ODOM, O. W., Johnson, C. H., & Herrin, D. L. (1999) · Molecular and General Genetics MGG · doi:10.1007/s004380051101 · Cited by 64 · see citing works

DNA transfer from chloroplast to nucleus is much rarer in Chlamydomonas than in tobacco

Lister, D. L., Bateman, J. E., Purton, S., & Howe, C. J. (2003) · Gene · doi:10.1016/s0378-1119(03)00754-6 · Cited by 59 · see citing works

Minimal Extent of Sequence Homology Required for Homologous Recombination at the psbA Locus in Chlamydomonas reinhardtii Chloroplasts using PCR-generated DNA Fragments

Dauvillée, D., Hilbig, L., Preiß, S., & Johanningmeier, U. (2004) · Photosynthesis Research · doi:10.1023/b:pres.0000015384.24958.a9 · Cited by 51 · see citing works

Evidence for in vivo modulation of chloroplast RNA stability by 3′-UTR homopolymeric tails in Chlamydomonas reinhardtii

Komine, Y., Kikis, E. A., Schuster, G., & Stern, D. B. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.052327599 · Cited by 50 · see citing works

Recombination and Heterologous Expression of Allophycocyanin Gene in the Chloroplast of Chlamydomonas reinhardtii

Su, Z., Qian, K., Tan, C.-P., Meng, C., & Qin, S. (2005) · Acta Biochimica et Biophysica Sinica · doi:10.1111/j.1745-7270.2005.00092.x · Cited by 36 · see citing works

Introduction of the Archaebacterial Geranylgeranyl Pyrophosphate Synthase Gene into Chlamydomonas reinhardtii Chloroplast

Fukusaki, E., Nishikawa, T., Kato, K., Shinmyō, A., Hemmi, H., Nishino, T., & Kobayashi, A. (2003) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(03)80030-0 · Cited by 24 · see citing works

Engineering the Chloroplast Encoded Proteins of Chlamydomonas

Xiong, L. H., & Sayre, R. T. (2004) · Photosynthesis Research · doi:10.1023/b:pres.0000030458.98624.37 · Cited by 24 · see citing works

Regulation of Chloroplast Translation

Hauser, C. R., Gillham, N. W., & Boynton, J. E. (2006) · Kluwer Academic Publishers eBooks · doi:10.1007/0-306-48204-5_12 · Cited by 17 · see citing works

Functional Analysis of Plastid Genes through Chloroplast Reverse Genetics in Chlamydomonas

Rochaix, J. (2003) · Advances in photosynthesis and respiration · doi:10.1007/978-94-007-1038-2_5 · Cited by 15 · see citing works

Chloroplast Transformation and Reverse Genetics

Goldschmidt‐Clermont, M. (2006) · Kluwer Academic Publishers eBooks · doi:10.1007/0-306-48204-5_8 · Cited by 11 · see citing works

Chloroplast Transformation: Current Results and Future Prospects

Erickson, J. M. (2005) · Kluwer Academic Publishers eBooks · doi:10.1007/0-306-48127-8_32 · Cited by 9 · see citing works

Use of aminoglycoside adenyltransferase translational fusions to determine topology of thylakoid membrane proteins

Franklin, J. L., Zhang, J., & Redding, K. (2003) · FEBS Letters · doi:10.1016/s0014-5793(03)00034-6 · Cited by 7 · see citing works

Biofuels from algae: Challenges and potential

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Microalgae–nutritious, sustainable aqua-and animal feed source

Dineshbabu, G., Goswami, G., Kumar, R., Sinha, A., & Das, D. (2019) · Journal of Functional Foods · doi:10.1016/j.jff.2019.103545 · Cited by 285 · see citing works

Production of carotenoids by microalgae: achievements and challenges

Varela, J. C. S., Pereira, H., Vilà, M., & León, R. (2015) · Photosynthesis Research · doi:10.1007/s11120-015-0149-2 · Cited by 252 · see citing works

Transgene Expression in Microalgae—From Tools to Applications

Doron, L., Segal, N., & Shapira, M. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.00505 · Cited by 235 · see citing works

The algal chloroplast as a synthetic biology platform for production of therapeutic proteins

Dyo, Y. M., & Purton, S. (2018) · Microbiology · doi:10.1099/mic.0.000599 · Cited by 140 · see citing works

Biotechnological exploitation of microalgae

Gangl, D., Zedler, J. A. Z., Rajakumar, P. D., Ramos‐Martinez, E. M., Riseley, A. S., Włodarczyk, A., Purton, S., Sakuragi, Y., Howe, C. J., Jensen, P. E., et al. (2015) · Journal of Experimental Botany · doi:10.1093/jxb/erv426 · Cited by 145 · see citing works

Over-accumulation of astaxanthin in Haematococcus pluvialis through chloroplast genetic engineering

Galarza, J. I., Gimpel, J. A., Rojas, V., Arredondo-Vega, B. O., & Henríquez, V. (2018) · Algal Research · doi:10.1016/j.algal.2018.02.024 · Cited by 143 · see citing works

New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii

Brocklehurst, T. W., Waterhouse, J. C., Young, R., Economou, C. K., & Purton, S. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7354-6 · Cited by 116 · see citing works

The Dunaliella salina organelle genomes: large sequences, inflated with intronic and intergenic DNA

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Low Oxygen Response Mechanisms in Green Organisms

Banti, V., Giuntoli, B., Gonzali, S., Loreti, E., Magneschi, L., Novi, G., Paparelli, E., Parlanti, S., Pucciariello, C., Santaniello, A., et al. (2013) · International Journal of Molecular Sciences · doi:10.3390/ijms14034734 · Cited by 109 · see citing works

Multiple facets of anoxic metabolism and hydrogen production in the unicellular green alga Chlamydomonas reinhardtii

Grossman, A. R., Catalanotti, C., Yang, W., Dubini, A., Magneschi, L., Subramanian, V., Posewitz, M. C., & Seibert, M. (2010) · New Phytologist · doi:10.1111/j.1469-8137.2010.03534.x · Cited by 106 · see citing works

Green biologics: the algal chloroplast as a platform for making biopharmaceuticals

Taunt, H. N., Stoffels, L., & Purton, S. (2018) · Bioengineered · doi:10.1080/21655979.2017.1377867 · Cited by 81 · see citing works

Extending the biosynthetic repertoires of cyanobacteria and chloroplasts

Nielsen, A. Z., Mellor, S. B., Vavitsas, K., Włodarczyk, A., Gnanasekaran, T., de Jesus, M. P. R. H., King, B. C., Bakowski, K., & Jensen, P. E. (2016) · The Plant Journal · doi:10.1111/tpj.13173 · Cited by 79 · see citing works

Genetic engineering of algal chloroplasts: progress and prospects

Purton, S., Szaub, J. B., Wannathong, T., Young, R., & Economou, C. K. (2013) · Russian Journal of Plant Physiology · doi:10.1134/s1021443713040146 · Cited by 74 · see citing works

Algae after dark: mechanisms to cope with anoxic/hypoxic conditions

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A Simple, Low-Cost Method for Chloroplast Transformation of the Green Alga Chlamydomonas reinhardtii

Economou, C. K., Brocklehurst, T. W., Szaub, J., & Purton, S. (2014) · Methods in molecular biology · doi:10.1007/978-1-62703-995-6_27 · Cited by 72 · see citing works

CHLOROPLAST GENETIC TOOL FOR THE GREEN MICROALGAE HAEMATOCOCCUS PLUVIALIS (CHLOROPHYCEAE, VOLVOCALES) 1

Gutiérrez, C. L., Gimpel, J. A., Escobar, C., Marshall, S. H., & Henríquez, V. (2012) · Journal of Phycology · doi:10.1111/j.1529-8817.2012.01178.x · Cited by 64 · see citing works

Selectable Markers and Reporter Genes for Engineering the Chloroplast of Chlamydomonas reinhardtii

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DIRECTED EVOLUTION: SELECTION OF THE HOST ORGANISM

Pourmir, A., & Johannes, T. W. (2012) · Computational and Structural Biotechnology Journal · doi:10.5936/csbj.201209012 · Cited by 57 · see citing works

Cytosine deaminase as a negative selectable marker for the microalgal chloroplast: a strategy for the isolation of nuclear mutations that affect chloroplast gene expression

Young, R., & Purton, S. (2014) · The Plant Journal · doi:10.1111/tpj.12675 · Cited by 51 · see citing works

A rapid, modular and marker-free chloroplast expression system for the green alga Chlamydomonas reinhardtii

Bertalan, I., Munder, M. C., Weiß, C., Kopf, J., Fischer, D., & Johanningmeier, U. (2014) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2014.12.017 · Cited by 49 · see citing works

Engineering microalgae through chloroplast transformation to produce high‐value industrial products

Siddiqui, A., Wei, Z., Boehm, M., & Ahmad, N. (2019) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.1823 · Cited by 48 · see citing works

Genomics of Volvocine Algae

Umen, J., & Olson, B. J. S. C. (2012) · Advances in botanical research · doi:10.1016/b978-0-12-391499-6.00006-2 · Cited by 46 · see citing works

An energy balance from absorbed photons to new biomass for Chlamydomonas reinhardtii and Chlamydomonas acidophila under neutral and extremely acidic growth conditions

Langner, U., Jakob, T., Stehfest, K., & Wilhelm, C. (2008) · Plant Cell & Environment · doi:10.1111/j.1365-3040.2008.01917.x · Cited by 41 · see citing works

The ARG9 Gene Encodes the Plastid-Resident N -Acetyl Ornithine Aminotransferase in the Green Alga Chlamydomonas reinhardtii

Remacle, C., Cline, S. G., Boutaffala, L., Gabilly, S. T., Larosa, V., Barbieri, M. R., Coosemans, N., & Hamel, P. P. (2009) · Eukaryotic Cell · doi:10.1128/ec.00108-09 · Cited by 38 · see citing works

Introducing pyruvate oxidase into the chloroplast of Chlamydomonas reinhardtii increases oxygen consumption and promotes hydrogen production

Xu, F.-Q., Ma, W., & Zhu, X. (2011) · International Journal of Hydrogen Energy · doi:10.1016/j.ijhydene.2011.05.130 · Cited by 39 · see citing works

The algal chloroplast as a testbed for synthetic biology designs aimed at radically rewiring plant metabolism

Jackson, H. O., Taunt, H. N., Mordaka, P. M., Smith, A. G., & Purton, S. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.708370 · Cited by 37 · see citing works

Overexpression of a MYB1 Transcription Factor Enhances Triacylglycerol and Starch Accumulation and Biomass Production in the Green Microalga Chlamydomonas reinhardtii

Zhao, J., Ge, Y., Liu, K., Yamaoka, Y., Zhang, D., Chi, Z., Akkaya, M. S., & Kong, F. (2023) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.3c05290 · Cited by 36 · see citing works

Marker‐free genetic engineering of the chloroplast in the green microalga C hlamydomonas reinhardtii

Chen, H.-C., & Melis, A. (2013) · Plant Biotechnology Journal · doi:10.1111/pbi.12073 · Cited by 36 · see citing works

Localized control of oxidized RNA

Zhan, Y., Dhaliwal, J. S., Adjibade, P., Uniacke, J., Mazrouï, R., & Zerges, W. (2015) · Journal of Cell Science · doi:10.1242/jcs.175232 · Cited by 36 · see citing works

Multigenic engineering of the chloroplast genome in the green alga Chlamydomonas reinhardtii

Larrea-Álvarez, M., & Purton, S. (2020) · Microbiology · doi:10.1099/mic.0.000910 · Cited by 34 · see citing works

Manipulation of the microalgal chloroplast by genetic engineering for biotechnological utilization as a green biofactory

Kwon, Y. M., Kim, K. W., Choi, T. Y., Kim, S. Y., & Kim, J. Y. H. (2018) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-018-2567-8 · Cited by 31 · see citing works

Expression of Anti-Lipopolysaccharide Factor Isoform 3 in Chlamydomonas reinhardtii Showing High Antimicrobial Activity

Li, A., Huang, R., Wang, C., Hu, Q., Li, H., & Xiao, L. (2021) · Marine Drugs · doi:10.3390/md19050239 · Cited by 30 · see citing works

Method to assemble and integrate biochemical pathways into the chloroplast genome of Chlamydomonas reinhardtii

Noor‐Mohammadi, S., Pourmir, A., & Johannes, T. W. (2012) · Biotechnology and Bioengineering · doi:10.1002/bit.24569 · Cited by 29 · see citing works

Improving recombinant protein production in the Chlamydomonas reinhardtii chloroplast using vivid Verde Fluorescent Protein as a reporter

Braun‐Galleani, S., Baganz, F., & Purton, S. (2015) · Biotechnology Journal · doi:10.1002/biot.201400566 · Cited by 28 · see citing works

Recent biotechnological developments in reshaping the microalgal genome: A signal for green recovery in biorefinery practices

Singh, M., Mal, N., Mohapatra, R., Bagchi, T., Parambath, S. D., Chavali, M. S., Rao, K. M., Ramanaiah, S. V., Kadier, A., Kumar, G., et al. (2022) · Chemosphere · doi:10.1016/j.chemosphere.2022.133513 · Cited by 25 · see citing works

Characterisation of a simple ‘hanging bag’ photobioreactor for low‐cost cultivation of microalgae

Cui, J., Purton, S., & Baganz, F. (2021) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.6985 · Cited by 25 · see citing works

Genetic Engineering to Improve Algal Biofuels Production

Rasala, B. A., Gimpel, J. A., Tran, M., Hannon, M. J., Miyake-Stoner, S. J., Specht, E. A., & Mayfield, S. P. (2012) · Springer eBooks · doi:10.1007/978-94-007-5479-9_6 · Cited by 24 · see citing works

Biosafety and the Environmental Uses of Micro-Organisms

OECD (2014) · Harmonisation of regulatory oversight in biotechnology · doi:10.1787/9789264213562-en · Cited by 24 · see citing works

Genetic Engineering for Microalgae Strain Improvement in Relation to Biocrude Production Systems

Stephens, E., Wolf, J., Oey, M., Zhang, E., Hankamer, B. D., & Ross, I. L. (2015) · Biofuel and biorefinery technologies · doi:10.1007/978-3-319-16640-7_11 · Cited by 22 · see citing works

Transgenic Microalgae Expressing Double-Stranded RNA as Potential Feed Supplements for Controlling White Spot Syndrome in Shrimp Aquaculture

Charoonnart, P., Taunt, H. N., Yang, L., Webb, C., Purton, S., Robinson, C., & Saksmerprome, V. (2023) · Microorganisms · doi:10.3390/microorganisms11081893 · Cited by 20 · see citing works

A microalgal‐based preparation with synergistic cellulolytic and detoxifying action towards chemical‐treated lignocellulose

Benedetti, M., Barera, S., Longoni, P., Guardini, Z., García, N. H., Bolzonella, D., López-Arredondo, D. L., Herrera‐Estrella, L., Goldschmidt‐Clermont, M., Bassi, R., et al. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13447 · Cited by 19 · see citing works

Novel Sulfated Polysaccharides of Red Microalgae: Basics and Applications

Arad, S. M., & van Moppes, D. (2013) · doi:10.1002/9781118567166.ch21 · Cited by 19 · see citing works

A highly active histidine-tagged Chlamydomonas reinhardtii Photosystem II preparation for structural and biophysical analysis

Cullen, M. D., Ray, N., Husain, S., Nugent, J. H. A., Nield, J., & Purton, S. (2007) · Photochemical & Photobiological Sciences · doi:10.1039/b708611n · Cited by 17 · see citing works

Algal Biotechnology

Borowitzka, M. A. (2015) · Cellular origin, life in extreme habitats and astrobiology · doi:10.1007/978-94-017-7321-8_11 · Cited by 17 · see citing works

CpPosNeg: A positive‐negative selection strategy allowing multiple cycles of marker‐free engineering of the Chlamydomonas plastome

Jackson, H. O., Taunt, H. N., Mordaka, P. M., Kumari, S., Smith, A. G., & Purton, S. (2022) · Biotechnology Journal · doi:10.1002/biot.202200088 · Cited by 16 · see citing works

Red Microalgae: From Basic Know-How to Biotechnology

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Harnessing Algal Peroxisomes for Efficient Poly Hydroxybutyrate Production

Zhang, H., Ma, S., Dou, X., Chen, R., Lü, H., Chi, Z., Xue, S., Li‐Beisson, Y., & Kong, F. (2024) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.3c07974 · Cited by 12 · see citing works

Magnetic separation of algae genetically modified for increased intracellular iron uptake

Buck, A. C., Moore, L. R., Lane, C. D., Kumar, A., Stroff, C., White, N. M., Xue, W., Chalmers, J. J., & Zborowski, M. (2014) · Journal of Magnetism and Magnetic Materials · doi:10.1016/j.jmmm.2014.09.008 · Cited by 11 · see citing works

Regulation of nucleus‐encoded trans‐acting factors allows orthogonal fine‐tuning of multiple transgenes in the chloroplast of Chlamydomonas reinhardtii

Mordaka, P. M., Clouston, K., Rovira, A. G., Sutherland, C., Zhang, D., Geisler, K., Mehrshahi, P., & Smith, A. G. (2024) · Plant Biotechnology Journal · doi:10.1111/pbi.14557 · Cited by 10 · see citing works

Synthesis of Recombinant Products in the Chloroplast

Tissot, G., Purton, S., Dubald, M., & Goldschmidt‐Clermont, M. (2014) · Springer eBooks · doi:10.1007/978-1-4939-1136-3_18 · Cited by 9 · see citing works

Algae, a New Biomass Resource

Formighieri, C., & Bassi, R. (2013) · Springer eBooks · doi:10.1007/978-1-4614-5820-3_436 · Cited by 7 · see citing works

N-terminal sequences affect expression of triterpene biosynthesis enzymes in Chlamydomonas chloroplasts

Hsu, S., Browne, D. R., Tatli, M., Devarenne, T. P., & Stern, D. B. (2019) · Algal Research · doi:10.1016/j.algal.2019.101662 · Cited by 7 · see citing works

Genetic Engineering Approaches for Algae

Rosales‐Mendoza, S. (2016) · Springer eBooks · doi:10.1007/978-3-319-32232-2_2 · Cited by 7 · see citing works

Algae: A New Biomass Resource

Pinnola, A., Formighieri, C., & Bassi, R. (2017) · Springer eBooks · doi:10.1007/978-1-4939-2493-6_436-3 · Cited by 7 · see citing works

New Synthetic Operon Vectors for Expressing Multiple Proteins in the Chlamydomonas reinhardtii Chloroplast

Yeon, J., Miller, S. M., & Dejtisakdi, W. (2023) · Genes · doi:10.3390/genes14020368 · Cited by 6 · see citing works

Genetic transformation of Chlamydomonas nuclear, chloroplast, and mitochondrial genomes

Weeks, D. P. (2023) · Elsevier eBooks · doi:10.1016/b978-0-12-822457-1.00018-2 · Cited by 6 · see citing works

Accelerating Chloroplast Engineering: A New System for Rapid Generation of Marker-Free Transplastomic Lines of Chlamydomonas reinhardtii

Taunt, H. N., Jackson, H. O., Gunnarsson, Í. N., Pervaiz, R., & Purton, S. (2023) · Microorganisms · doi:10.3390/microorganisms11081967 · Cited by 6 · see citing works

Chloroplast expression of myoglobin and functional characterization of C. reinhardtii biomass for alternative meat applications

Phadnis, G., & Prakash, G. (2024) · Algal Research · doi:10.1016/j.algal.2024.103807 · Cited by 5 · see citing works

Harnessing microalgae for bioproducts: innovations in synthetic biology

Li, Z., Cheng, Y., Li, C., Wu, Q., & Xin, Y. (2025) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-025-04727-7 · Cited by 4 · see citing works

Chlamydomonas reinhardtii for the production of recombinant proteins: current knowledge and perspectives

Quezada-Rivera, J. J., Ponce-Alonso, J., Dávalos-Guzmán, S. D., & Soria‐Guerra, R. E. (2024) · Elsevier eBooks · doi:10.1016/b978-0-323-98388-4.00010-1 · Cited by 4 · see citing works

Estimating the Maximum Achievable Productivity in Outdoor Ponds: Microalgae Biomass Growth Modeling and Climate-Simulated Culturing

Huesemann, M. H., Wigmosta, M. S., Crowe, B., Waller, P. M., Chavis, A., Hobbs, S. J., Edmundson, S. J., Chubukov, B. A., Tocco, V., & Coleman, A. M. (2016) · CRC Press eBooks · doi:10.1201/b19464-6 · Cited by 4 · see citing works

Microbial-based cleaning products in use and the potential role of transgenic micro-organisms

OECD (2015) · Harmonisation of regulatory oversight in biotechnology · doi:10.1787/9789264213562-13-en · Cited by 4 · see citing works

Metabolic Engineering of Selected Secondary Metabolites

Ludwig‐Müller, J. (2014) · Springer eBooks · doi:10.1007/978-94-017-9223-3_21 · Cited by 3 · see citing works

Plastid Transformation in Algae

Nickelsen, J., & Bohne, A. (2012) · Advances in photosynthesis and respiration · doi:10.1007/978-94-007-2920-9_16 · Cited by 3 · see citing works

Recent Insights Into Algal Biotechnology

Parkavi, K., Raja, R., Arunkumar, K., Coelho, A., Hemaiswarya, S., & Carvalho, I. S. (2020) · Wiley eBooks · doi:10.1002/9781119143802.ch19 · Cited by 3 · see citing works

Algal biomass pretreatment and developments for better biofuel production

Mateescu, C. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-824270-4.00007-9 · Cited by 2 · see citing works

Strategies to Study Dark Growth Deficient or Slower Mutants in Chlamydomonas reinhardtii

Yang, H., Han, F., Wang, Y., Yang, W., & Tu, W. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1370-2_13 · Cited by 2 · see citing works

Algae: A New Biomass Resource

Pinnola, A., Formighieri, C., & Bassi, R. (2018) · Springer eBooks · doi:10.1007/978-1-4939-7813-7_436 · Cited by 2 · see citing works

Lessons of the impact of genetically engineered micro-organisms on natural ecosystems like soil

OECD (2015) · Harmonisation of regulatory oversight in biotechnology · doi:10.1787/9789264213562-7-en · Cited by 2 · see citing works

Phytosanitation and the development of biocontrol agents

OECD (2015) · Harmonisation of regulatory oversight in biotechnology · doi:10.1787/9789264213562-6-en · Cited by 2 · see citing works

Algae, a New Biomass Resource

Formighieri, C., & Bassi, R. (2012) · Springer eBooks · doi:10.1007/978-1-4419-0851-3_436 · Cited by 2 · see citing works

Genetic Engineering of Microalgae: Current Status and Future Prospects

Spicer, A., & Purton, S. (2016) · Microalgal Production for Biomass and High-Value Products · doi:10.1201/b19464-7 · Cited by 17 · see citing works

Development of chloroplast engineering tools for Asterarcys sp.: A resilient scenedesmaceae microalga

Kumari, S., Lali, A. M., & Prakash, G. (2022) · Algal Research · doi:10.1016/j.algal.2022.102770 · Cited by 1 · see citing works

Genetic Engineering of Plants for Remediation of Toxic Metals and Metalloids

Nisa, S. G., Hameed, M., Eman, I., Fatima, N., Sadiq, S., Rafique, S., Khan, Z., & Shah, T. M. (2025) · CABI biotechnology series. · doi:10.1079/9781800627659.0013 · Cited by 1 · see citing works

Engineering bidirectional chloroplast promoters for tunable co-expression of multiple genes in microalgae (Chlamydomonas reinhardtii)

Tazon, A. W., Mérindol, N., Fantino, E. I., Bouhadada, A., Awwad, F., Meddeb-Mouelhi, F., & Desgagné‐Penix, I. (2026) · Communications Biology · doi:10.1038/s42003-025-09478-7 · Cited by 2 · see citing works

Reflection on environmental risk assessment of micro-organisms

OECD (2015) · Harmonisation of regulatory oversight in biotechnology · doi:10.1787/9789264213562-18-en · Cited by 1 · see citing works

INSIGHT INTO CRISPR SYSTEM IN EUKARYOTIC MICROALGAE, A REVIEW

Bilal, I., Butnariu, M., & Bilal, E. (2019) · EurasianUnionofScientists · doi:10.31618/esu.2413-9335.2019.3.59.37-48 · Cited by 1 · see citing works

The intentional release of micro-organisms into the environment

OECD (2015) · Harmonisation of regulatory oversight in biotechnology · doi:10.1787/9789264213562-12-en · Cited by 1 · see citing works

Expression of a mosquito larvicidal gene in chloroplast and nuclear compartments of Chlamydomonas reinhardtii

Pervaiz, R., Khan, M. A., Raza, F. A., Ahmad, S., Zafar, A. U., Ahmed, N., & Akram, M. (2022) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2022.11.004 · Cited by 1 · see citing works

MAKING CHLAMYDOMONAS REINHARDTII A BETTER MODEL ORGANISM: TACKLING THE INEFFICIENCY OF NUCLEAR TRANSGENE EXPRESSION AND IMPROVING METHODS FOR THE GENERATION AND CHARACTERIZATION OF INSERTIONAL MUTANT LIBRARIES

Plucinak, T. M. (2013) · Lincoln (University of Nebraska) · Cited by 1 · see citing works

Expression of the bacterial ectA gene in the chloroplast of Chlamydomonas reinhardtii

Lunde, A. (2012) · NORA - Norwegian Open Research Archives · Cited by 1 · see citing works

Plastid Transformation

Hogg, B. V., Lelivelt, C. L. C., Dunne, A., Nguyen, K. L. T., & Nugent, J. M. (2010) · doi:10.1002/9780470686522.ch13 · Cited by 1 · see citing works

Expression of the bacterial ectC gene in the chloroplast of Chlamydomonas reinhardtii

Holck, V. T. (2014) · NORA - Norwegian Open Research Archives

Metabolic engineering of the algal chloroplast for terpenoid production

Hoqani, U. A. (2017) · UCL Discovery (University College London)

The benefits and advantages of commercial algal biomass harvesting

OECD (2015) · Harmonisation of regulatory oversight in biotechnology · doi:10.1787/9789264213562-9-en

Designing bacteria for the environment

(2015) · Harmonisation of regulatory oversight in biotechnology · doi:10.1787/9789264213562-11-en

Overarching issues in the environmental risk assessment of deliberate release of transgenic micro-organisms

OECD (2015) · Harmonisation of regulatory oversight in biotechnology · doi:10.1787/9789264213562-20-en

References to Volume 1

(2009) · Elsevier eBooks · doi:10.1016/b978-0-12-370873-1.00054-x

Use and release of mosquitoes for the control of dengue transmission

OECD (2015) · Harmonisation of regulatory oversight in biotechnology · doi:10.1787/9789264213562-15-en

Foreword Acknowledgements

OECD (2015) · Harmonisation of regulatory oversight in biotechnology · doi:10.1787/9789264213562-1-en

Regulation of photosynthetic cyclic electron transport and photo-production of hydrogen in Chlamydomonas reinhardtii

Dang, T. K. V. (2015) · doi:10.70675/3080146bz46a9z4387za2fcze667fa52bbf7

A Simple, Low-Cost Method for Chloroplast Transformation of the Green Alga Chlamydomonas reinhardtii

Economou, C. K., Brocklehurst, T. W., Szaub, J., & Purton, S. (2014) · Methods in molecular biology · doi:10.1007/978-1-62703-995-6_27 · Cited by 72 · see citing works

Plastid Transformation in Algae

Nickelsen, J., & Bohne, A. (2012) · Advances in photosynthesis and respiration · doi:10.1007/978-94-007-2920-9_16 · Cited by 3 · see citing works

A Simple Technology for Generating Marker-Free Chloroplast Transformants of the Green Alga Chlamydomonas reinhardtii

Larrea-Álvarez, M., Young, R., & Purton, S. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1472-3_17 · Cited by 12 · see citing works

Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas reinhardtii

Guzmán-Zapata, D., Macedo-Osorio, K. S., Almaraz-Delgado, A. L., Durán‐Figueroa, N., & Badillo-Corona, J. A. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-3289-4_5 · Cited by 17 · see citing works

Chloroplast Transformation: Current Results and Future Prospects

Erickson, J. M. (2005) · Kluwer Academic Publishers eBooks · doi:10.1007/0-306-48127-8_32 · Cited by 9 · see citing works