Cardiac Tissue Engineering Models of Inherited and Acquired Cardiomyopathies
Open accessAbstract
The lack of biomimetic in vitro models of the human heart has posed a critical barrier to progress in the field of modeling cardiac disease. Human engineered cardiac tissues (hECTs)-autonomous, beating structures that recapitulate key aspects of native cardiac muscle physiology-offer an attractive alternative to traditional in vitro models. Here we describe the use of hECTs to advance our understanding and modeling of cardiac diseases in order to test therapeutic interventions, with a focus on contractile dysfunction in the setting of inherited and acquired forms of cardiomyopathies. Four major procedures are discussed in this chapter: (1) preparation of hECTs from human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) on single-tissue and multitissue bioreactors; (2) data acquisition of hECT contractile function on both of these platforms; (3) hECT modeling of hereditary phospholamban-R14 deletion-dilated cardiomyopathy; and (4) cryo-injury and doxorubicin-induced hECT models of acquired cardiomyopathy.
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Bioengineered cardiac tissues, created from human pluripotent stem cell (hPSC)-derived cardiomyocytes, have successfully modeled aspects of native human heart structure and function, including cardiotoxic and therapeutic responses, and phenotypic characteristics of genetic and acquired cardiomyopathies.
“Bioengineered cardiac tissues, created from human pluripotent stem cell (hPSC)derived cardiomyocytes, have successfully modeled aspects of native human heart structure and function, 16 including cardiotoxic and therapeutic responses, 17,18 and phenotypic characteristics of genetic and acquired cardiomyopathies.19,20”
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- Authors
- Turnbull, I. C., Mayourian, J., Murphy, J. F., Stillitano, F., Ceholski, D. K., & Costa, K. D.
- Affiliations
- Icahn School of Medicine at Mount Sinai (US)
- Corresponding author
- Costa, Kevin D.
- ORCID
- Venue
- Methods in molecular biology
- Publisher
- Springer New York
- Published
- 2018
- Volume
- 1816
- Pages
- 145-159
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- PMC
- PMC6561092
- OpenAlex
- W2844037327
- Cited by
- 26
- References
- 11
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 5 · 2022: 3 · 2021: 5 · 2020: 4 · 2019: 2 · 2018: 1
- FWCI
- 4.03
- Type
- book chapter
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- American Heart Association (15POST25090116)
- National Institutes of Health
- National Heart, Lung, and Blood Institute (F30HL134283, R01HL132226, K01HL133424)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiomyopathy and Myosin Studies
- Keywords
- engineered heart tissue, human induced pluripotent stem cell-derived cardiomyocytes, cardiomyopathy, familial cardiomyopathy, acquired cardiomyopathy, contractile dysfunction, dilated cardiomyopathy, cryoinjury, doxorubicin cardiotoxicity, bioreactors, therapeutic testing
- MeSH terms
- Cardiomyopathy, Dilated, Cell Differentiation, Doxorubicin, Equipment Design, Freezing, Humans, Myocardial Contraction, Cardiomyopathies, Myocardium, Bioreactors, Tissue Engineering, Myocytes, Cardiac, Induced Pluripotent Stem Cells
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