The DOI matches its Crossref record.
Cryopreservation and Freeze-Drying Protocols
Abstract
Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995. This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.
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“In fact, Nostoc muscorum was successfully lyophilized to test this concept, and it was found to remain viable after five years [84].”
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Items 301–313 of 313
Paredes, E. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-809633-8.20629-8
(2019) · Elsevier eBooks · doi:10.1016/b978-0-12-812465-9.00013-x
Process Technologies of Cyanobacteria
Witthohn, M., Strieth, D., Kollmen, J., Schwarz, A. M., Ulber, R., & Muffler, K. (2022) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2022_214
Gorordo, M. F., Lucca, M. E., & Sangorrín, M. P. (2022) · SSRN Electronic Journal · doi:10.2139/ssrn.4259988
Smith, D., Ryan, M. J., & Buddie, A. (2023) · CABI eBooks · doi:10.1079/9781800622135.0004
Cryopreservation of six marine microalgae used in Taiwanese aquaculture
Gwo, J., Twan, W., & Lin, S. (2023) · Journal of the World Aquaculture Society · doi:10.1111/jwas.12957
Sari, M., & Idris, M. (2024) · JURNAL BIOLOGI TROPIS · doi:10.29303/jbt.v24i2.6651
Orchid Cryopreservation Via Protocorms
Soonthornkalump, S., Yamamoto, S., & Thammasiri, K. (2024) · Springer protocols handbooks/Springer protocols · doi:10.1007/978-1-0716-4031-9_12
IUCN Species Survival Commission biobanking guidelines for conservation purposes
Hvilsom, C., Brown, A., Bruford, M., Berner, J., Boettcher, P., Bragg, J., Calatayud, N., Costa, M., Daly, J., Dulloo, M. E., et al. (2025) · doi:10.2305/eclr4913 · Cited by 1
Vieillissement et maladies neurodégénératives : nouvelles contraintes apportées par la métallomique
Sauzéat, L. (2018) · theses.fr (ABES) · doi:10.70675/6f22af22z8cfcz4a9fzb647zf065e4131641
Gorordo, M. F., Lucca, M. E., & Sangorrín, M. P. (2026) · Current Microbiology · doi:10.1007/s00284-026-05053-3
Miyanmahaleh, Y., Hosseini, F., Hogan, S. A., Stanton, C., & Ross, R. P. (2026) · Applied Food Research · doi:10.1016/j.afres.2026.102569
Metadata
- Authors
- Day, J., & Stacey, G.
- Affiliations
- National Institute for Biological Standards and Control (GB)
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2007
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W1506233354
- Cited by
- 318
- References
- 0
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 11 · 2023: 15 · 2022: 15 · 2021: 21 · 2020: 16 · 2019: 19 · 2018: 21 · 2017: 25 · 2016: 14 · 2015: 17 · 2014: 16 · 2013: 23 · 2012: 27
- FWCI
- 13.41
- Type
- book
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Methods in molecular biology (publishedVersion)
- DIAL (Catholic University of Leuven) (submittedVersion)
- Funders
- NHS Blood and Transplant
- University of Bedfordshire
- Cardiff University
- Directorate for Biological Sciences
- Biotechnology and Biological Sciences Research Council
- Universitätsklinikum Hamburg-Eppendorf
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
- Keywords
- cryopreservation, freeze-drying, ex situ conservation, biological resource centers, protein preservation, long-term preservation
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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