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Cryopreservation and Freeze-Drying Protocols
Abstract
Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995. This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.
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“In fact, Nostoc muscorum was successfully lyophilized to test this concept, and it was found to remain viable after five years [84].”
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Items 101–200 of 313
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“There was no gradual control of temperature in cryopreservation tank in this study.(14,25) Furthermore, in the process of thawing in the room temperature, the process of freezing is reversed.”
BackgroundMethodLong-term preservation of Leptospira spp.: challenges and prospects
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Recombinant peptide reverses cryo-capacitation in ram sperm and improves in vitro fertilization
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Cryopreservation of undifferentiated and differentiated human neuronal cells
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Gene Banking: The Freezing Strategy
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Effect of Marine-Derived Ice-Binding Proteins on the Cryopreservation of Marine Microalgae
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“The serial maintenance is not only costly, but is also likely to cause genetic drift, contamination, and the loss of their nutritional value as aquaculture feed [29,46,47].”
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Upton, R. M. O., Clulow, S., Colyvas, K., Mahony, M. J., & Clulow, J. (2023) · Reproduction · doi:10.1530/rep-22-0486 · Cited by 15
Viability of fastidious Phytophthora following different cryopreservation treatments
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“Advanced toxicity assessments allow measurements at single-cell level such as; 1) using fluorescein diacetate (FDA) (Agusti et al., 1998), and 2) Live/ Dead BacLight (Day and Stacey, 1995; Boulos et al., 1999; Mori et al., 2002).”
MethodImpact of Storage at −80°C on Encapsulated Liver Spheroids After Liquid Nitrogen Storage
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Cryopreservation of sea urchin sperm and early life stages
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Fundamental Points to Consider in the Cryopreservation and Shipment of Cells for Human Application
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Self-citationEffect of high DMSO concentration on albumin during freezing and vitrification
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Pollen Cryopreservation: Advances and Prospects
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“Proper tissue preservation from a wide range of animals of different species is of paramount importance, as these tissue samples could be used to reintroduce lost genes back into the breeding pool by somatic cloning (Day and Stacey 2007a, b).”
BackgroundSemen cryopreservation of yellow croaker Larimichthys polyactis
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Metadata
- Authors
- Day, J., & Stacey, G.
- Affiliations
- National Institute for Biological Standards and Control (GB)
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2007
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W1506233354
- Cited by
- 318
- References
- 0
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 11 · 2023: 15 · 2022: 15 · 2021: 21 · 2020: 16 · 2019: 19 · 2018: 21 · 2017: 25 · 2016: 14 · 2015: 17 · 2014: 16 · 2013: 23 · 2012: 27
- FWCI
- 13.41
- Type
- book
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Methods in molecular biology (publishedVersion)
- DIAL (Catholic University of Leuven) (submittedVersion)
- Funders
- NHS Blood and Transplant
- University of Bedfordshire
- Cardiff University
- Directorate for Biological Sciences
- Biotechnology and Biological Sciences Research Council
- Universitätsklinikum Hamburg-Eppendorf
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
- Keywords
- cryopreservation, freeze-drying, ex situ conservation, biological resource centers, protein preservation, long-term preservation
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