Cited by 318Reproductive Biology and FertilityMolecular Biology Techniques and ApplicationsPlant tissue culture and regeneration

The DOI matches its Crossref record.

Cryopreservation and Freeze-Drying Protocols

John Day, Glyn Stacey·2007·Methods in Molecular Biology

DOI 10.1007/978-1-59745-362-2

Open this paper in CoChat — free account

Abstract

Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995. This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.

Integrity signals

Registry

Crossref · 10.1007/978-1-59745-362-2

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Sorted by citations

Items 201–300 of 313

Cryopreservation of Plant Germplasm
Keller, E. R. J., & Senula, A. (2010) · doi:10.1002/9780470686522.ch8 · Cited by 8
Storage procedures for yeast preservation: phenotypic and genotypic evaluation
Sant’Ana, P. D. L., Gonçalves, R. B., & Höfling, J. F. (2007) · Annals of Microbiology · doi:10.1007/bf03175090 · Cited by 7
Effect of the desiccation tolerance and cryopreservation methods on the viability of Citrus limon L. Burm cv. Eureka seeds
Orjuela-Palacio, J. M., Graiver, N., Santos, M. V., et al. (2019) · Cryobiology · doi:10.1016/j.cryobiol.2019.05.006 · Cited by 7
Zinc ferrite nanoparticles: simple synthesis via lyophilisation and electrochemical application as glucose biosensor
Cao, X. (2021) · Nano Express · doi:10.1088/2632-959x/abfdd2 · Cited by 7
Protective Effects of Cryoprotectants and Lyoprotectants on the Survival of Persipeptide Producing Streptomyces zagrosensis UTMC 1154
Mohammadipanah, F., Parvizi, L., Hamedi, J., et al. (2017) · Biopreservation and Biobanking · doi:10.1089/bio.2017.0046 · Cited by 7
Natural deep eutectic systems—A new era of cryopreservation
Duarte, A. R. C., Meneses, L., Duarte, A. R. C., & Paiva, A. (2020) · Advances in botanical research · doi:10.1016/bs.abr.2020.09.015 · Cited by 7
Oral Staphylococcus Species and MRSA Strains in Patients with Orofacial Clefts Undergoing Surgical Rehabilitation Diagnosed by MALDI-TOF MS
Oliveira, M. C., Boriollo, M. F. G., de Souza, A. C., et al. (2024) · Pathogens · doi:10.3390/pathogens13090763 · Cited by 6
Medium-term cryopreservation of rabies virus samples
Aguiar, T. D. D. F., Teixeira, M. F. D. S., Costa, E. C., et al. (2013) · Revista da Sociedade Brasileira de Medicina Tropical · doi:10.1590/0037-8682-0135-2013 · Cited by 6
The Family Haloplasmataceae
Antunes, A. (2014) · Springer eBooks · doi:10.1007/978-3-642-30120-9_206 · Cited by 7
Algae at Extreme Low Temperatures
Benson, E. E., Harding, K., & Day, J. (2007) · Cellular origin and life in extreme habitats · doi:10.1007/978-1-4020-6112-7_19 · Cited by 6
Self-citation
Asymmetric reduction of a ketone by wet and lyophilized cells of Geotrichum candidum in organic solvents
Bhattacharyya, M. S., Singh, A., & Banerjee, U. C. (2011) · New Biotechnology · doi:10.1016/j.nbt.2011.07.005 · Cited by 6
Humidity and Deterioration Mechanisms
Camuffo, D. (2019) · Elsevier eBooks · doi:10.1016/b978-0-444-64106-9.00006-7 · Cited by 6
Monitoring of growth and biochemical composition of Dunaliella salina and Dunaliella polymorpha in different photobioreactors
Demirel, Z. (2022) · AQUATIC RESEARCH · doi:10.3153/ar22013 · Cited by 6
Cryopreservation of Plant Cells
Keller, E. R. J., Senula, A., Höfer, M., Heine‐Dobbernack, E., & Schumacher, H. M. (2013) · Encyclopedia of Industrial Biotechnology · doi:10.1002/9780470054581.eib244.pub2 · Cited by 6
Cryopreservation and recovery of a complex hypersaline microbial mat community
Bolhuis, H., & Grego, M. (2024) · Cryobiology · doi:10.1016/j.cryobiol.2024.104859 · Cited by 5
Impact of the Use of Cryoprotectants in the Production of Freeze-Dried Soluble Coffee from Cold Brew Arabica Coffee
Barroso, L. A., Viegas, C., Stančiauskaitė, M., Macedo, A. S., Lemos, I. L., da Costa, J. M. G., Schmiele, M. M., da Silveira, J. V. W., Brandão, P., Amaral, T. N., et al. (2024) · Food and Bioprocess Technology · doi:10.1007/s11947-024-03387-z · Cited by 5
Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods
Salas‐Leiva, J. S., & Dupré, E. (2011) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) · doi:10.4067/s0718-560x2011000200008 · Cited by 5
Innovative technology for microalgal cell preservation through immobilization in polylactic acid nanofibers
de Almeida, A. C. A., Vaz, B. D. S., Costa, J. A. V., & de Morais, M. G. (2024) · Algal Research · doi:10.1016/j.algal.2024.103781 · Cited by 5
The potential benefits of Lactobacillus farraginis and Enterococcus durans , isolated from kefir, as probiotic candidates on innate immune responses, expression of some immune genes, and resistance to Lactococcosis disease in juvenile rainbow trout
Ali, M., Soltanian, S., Mirghaed, A. T., Akhlaghi, M., & Hoseinifar, S. H. (2021) · Aquaculture Research · doi:10.1111/are.15419 · Cited by 5
Humidity and Conservation
Camuffo, D. (2013) · Elsevier eBooks · doi:10.1016/b978-0-444-63296-8.00003-2 · Cited by 5
Evaluation of Ethylene Glycol as a Cryoprotectant in the Sperm Cryopreservation of Trans-andean Shovelnose Catfish (Sorubim Cuspicaudus, Pimelodidae)
Atencio-García, V., Dorado, M. D. P., Navarro, E., et al. (2014) · Acta Biológica Colombiana · doi:10.15446/abc.v19n2.41288 · Cited by 5
Multiphoton imaging of freezing and heating effects in plant leaves
Breunig, H. G., Tümer, F., & König, K. (2012) · Journal of Biophotonics · doi:10.1002/jbio.201200093 · Cited by 5
“Understanding temperature-induced effects in plants is important for cryobiology and cryopreservation [1], furthermore, to investigate the response to external cooling and heating [2–5] and/or the associated microscopic effects on molecules such as chlorophyll [6, 7].”
Background
Culture Collections and Biological Resource Centres ( BRCs )
Smith, D. I. (2009) · Encyclopedia of Industrial Biotechnology · doi:10.1002/9780470054581.eib246 · Cited by 5
Slow-delivery and distributed exchange of cryoprotective agents with hydrogel beads
Sevenler, D., Bean, H., Toner, M., et al. (2021) · Cryobiology · doi:10.1016/j.cryobiol.2021.09.006 · Cited by 4
Ultra-Rapid Freezing Preserves Morphofunctional Integrity and Fertilizing Ability of Epididymal Cat Spermatozoa
Colombo, M., Morselli, M. G., Zahmel, J., et al. (2022) · Frontiers in Veterinary Science · doi:10.3389/fvets.2022.866953 · Cited by 4
“Indeed, cryoprotectants might be toxic (17), especially at higher temperatures (18), and they could cause osmotic stress (19), but modern vitrification approaches try to reduce or avoid their use.”
Background
Meet interesting abbreviations in clinical mass spectrometry: from compound classification by REIMS to multimodal and mass spectrometry imaging (MSI)
Luptáková, D., Pluháček, T., Palyzová, A., et al. (2017) · Acta Virologica · doi:10.4149/av_2017_316 · Cited by 4
“Cryo-preservation (Day and Stacey, 2007), heating and micro-waving (Mayers, 1970) are used among the usually applied physical methods.”
Method
The motility of Tor soro fish (Valenciennes, 1842) using post cryopreservation sperm: the effect of grape juice (Vitis vinifera) as a natural antioxidant
Anabella, N. A., Abinawanto, A., Subagja, J., et al. (2020) · IOP Conference Series Earth and Environmental Science · doi:10.1088/1755-1315/584/1/012063 · Cited by 4
Programmed Cooling and Vitrification Method Applied to the Germplasm Preservation of Eriobotrya Plants
Liu, Y., Huang, T., Lin, S., et al. (2023) · HortScience · doi:10.21273/hortsci16816-22 · Cited by 4
Immobilization of microbial multienzyme preparation on calcium alginate beads as well as lyophilization with mosambi peel matrix improved its shelf-life and stability
Singh, B., Soni, S. K., Vaish, S., et al. (2023) · Folia Microbiologica · doi:10.1007/s12223-023-01079-3 · Cited by 4
“Additionally, lyophilized enzymes can be kept for a significantly longer period for future usage (Day and Stacey 2007; Merivaara et al. 2022).”
Real time stability and viability prediction of the anticancer BCG after lyophilization
Mohamed, D., Elsayed, I., Mohamed, A. F., et al. (2015) · Drug Development and Industrial Pharmacy · doi:10.3109/03639045.2015.1004181 · Cited by 4
Histological evaluation of the repair process of replanted rat teeth after storage in resveratrol dissolved in dimethyl sulphoxide
Poi, W. R., Sonoda, C. K., Amaral, M. F., et al. (2018) · Dental Traumatology · doi:10.1111/edt.12402 · Cited by 4
Methods for the Ultrastructural Analysis of Plant Cells and Tissues
Dashek, W. V., & Mayfield, J. E. (2000) · Humana Press eBooks · doi:10.1007/978-1-59259-232-6_14 · Cited by 4
Investigation of membrane permeability of carp spermatozoa for water molecules
Puhovkin, A., Kopeika, E., Nardid, O. A., et al. (2014) · BIOPHYSICS · doi:10.1134/s000635091403018x · Cited by 4
Maintenance of Aquatic Hyphomycete Cultures
Marvanová, L. (2005) · Springer eBooks · doi:10.1007/1-4020-3466-0_20 · Cited by 4
Oxidative status and stress associated with cryopreservation of germplasm of recalcitrant-seeded species.
Naidoo, C. (2012) · ResearchSpace (University of KwaZulu-Natal) · Cited by 4
Ziziphus mauritiana (masau) fruits fermentation in Zimbabwe: from black-box to starter culture development
Nyanga, L. K. (2012) · doi:10.18174/239532 · Cited by 4
Genetic Transformation in Conifers
Minocha, S. C., & Minocha, R. (1999) · Forestry sciences · doi:10.1007/978-94-011-4774-3_17 · Cited by 4
The Impact of Precisely Controlled Pre-Freeze Cooling Rates on Post-Thaw Stallion Sperm
Bitton, A., Frishling, A., Kalo, D., et al. (2025) · Animals · doi:10.3390/ani16010021 · Cited by 3
Influence of Long-Term Agar-Slant Preservation at 4 °C on the Recombinant Enzyme Activity of Engineered Yeast
Liang, X., Gong, T., Chen, J., et al. (2023) · Fermentation · doi:10.3390/fermentation9020104 · Cited by 3
CRYOPRESERVATION OF HUMAN UMBILICAL CORD-DERIVED MESENCHYMAL STEM CELLS IN COMPLEX SUGAR BASED CRYOPROTECTIVE SOLUTIONS
Naaldijk, Y., Federova, V., & Stolzing, A. (2013) · Loughborough University Institutional Repository (Loughborough University) · Cited by 3
Preservation of Microbial Strains in the Wine Industry
Lopez, M. D. G., López-Coronado, J., López‐Ocaña, L., et al. (2011) · Elsevier eBooks · doi:10.1016/b978-0-12-375021-1.10012-8 · Cited by 3
Maintenance, Conservation and Regulation of Microbial Resources for Defense Applications
Sangwan, P., Sharma, K., Celin, M., et al. (2018) · Soil biology · doi:10.1007/978-3-319-96971-8_14 · Cited by 3
The numerical modeling of cell freezing in binary solution under subcooling conditions
Smakulski, P., Pietrowicz, S., & Ishimoto, J. (2019) · International Journal of Numerical Methods for Heat & Fluid Flow · doi:10.1108/hff-12-2018-0740 · Cited by 3
Comparison of different freezing rates on post-thaw viability, proliferation, and stemness of sheep spermatogonial stem cells
Binsila, B., Tomcy, T. A., Krishnappa, B., Sadikh, M., Ramachandran, N., Kolte, A. P., & Selvaraju, S. (2025) · Cryobiology · doi:10.1016/j.cryobiol.2025.105203 · Cited by 3
On the assessment of the stability of vitrified cryo-media by differential scanning calorimetry: A new tool for biobanks to derive standard operating procedures for storage, access and transport
Kreiner, A., Stracke, F., & Zimmermann, H. (2019) · Cryobiology · doi:10.1016/j.cryobiol.2019.06.002 · Cited by 3
Maintenance of Aquatic Hyphomycete Cultures
Marvanová, L. (2020) · Springer eBooks · doi:10.1007/978-3-030-30515-4_24 · Cited by 3
Slow growth in vitro conservation of Zizyphus jujuba Mill.
Sota, V., & Kongjika, E. (2014) · The Journal Agriculture and Forestry · Cited by 3
Cryopreservation of cyanobacteria
Day, J. (2013) · doi:10.1002/9781118402238.ch20 · Cited by 3
Self-citation
Long-term storage of microalgae: determination of optimum cryopreservation conditions
Deniz, İ., Demirel, Z., İmamoğlu, E., & Dalay, M. C. (2022) · Journal of the Marine Biological Association of the United Kingdom · doi:10.1017/s0025315422000479 · Cited by 3
Review on Cryoprotectant Utilization in Grouper Sperm Cryopreservation
Arsin, N. E. B., Zuldin, W. H., Chu, I. K. C., et al. (2025) · Reviews in Aquaculture · doi:10.1111/raq.70108 · Cited by 2
Heat and Mass Transfer Models and Measurements for Low-Temperature Storage of Biological Systems
Shaik, S. M., & Devireddy, R. V. (2018) · Springer eBooks · doi:10.1007/978-3-319-26695-4_73 · Cited by 2
Implications of a Catastrophic Refrigeration Failure on the Viability of Cryogenically Stored Samples
Day, J., Childs, K. H., & Stacey, G. N. (2022) · Protist · doi:10.1016/j.protis.2022.125915 · Cited by 3
Self-citation
Cryopreservation of Cat’s Claw (Uncaria tomentosa)
Pérez, J., Guillen, A., Ramos, K., Engelmann, F., & Abdelnour-Esquivel, A. (2015) · American Journal of Plant Sciences · doi:10.4236/ajps.2015.618291 · Cited by 2
Criopreservación de células estromales derivadas del tejido adiposo: comparación de diferentes medios de criopreservación
Pereira, N., González, E., & Caviedes, P. A. (2016) · Revista de Cirugía · doi:10.1016/j.rchic.2016.03.007 · Cited by 2
Template-based formation of ultrasound microbubble contrast agents
Huang, H., Lin, C. J., Chang, W. H., et al. (2016) · RSC Advances · doi:10.1039/c6ra09316g · Cited by 2
Cryopreservation in Tissue Banking
Kilbride, P., & Meneghel, J. (2021) · Springer eBooks · doi:10.1007/978-3-030-71621-9_8 · Cited by 2
Cryopreservation of Stem Cells
Chowdhury, S., & Ghosh, S. (2021) · Springer eBooks · doi:10.1007/978-981-16-1638-9_3 · Cited by 2
REVIEW OF MODERN METHODS OF CRYOPRESERVATION OF VARIOUS TYPES OF BIOLOGICAL MATERIAL
Заикина, Е. В., Goncharova, A. S., Pozdnyakova, V. V., et al. (2022) · Современные проблемы науки и образования · doi:10.17513/spno.31790 · Cited by 2
Novel method enabling a rapid vitality determination of cyanobacteria
Witthohn, M., Schwarz, A. M., Walther, J., et al. (2020) · Engineering in Life Sciences · doi:10.1002/elsc.201900164 · Cited by 2
Development of in-vitro culture and cryopreservation protocol for zebrafish (Danio rerio) ovarian tissue fragments
Anil, S. (2013) · University of Bedfordshire Repository (University of Bedfordshire) · Cited by 2
Aislamiento y caracterización molecular de bacteriófagos de bacterias enteropatógenas para biocontrol de enfermedades transmitidas por alimentos (ETA)
Dini, C. (2011) · doi:10.35537/10915/2686 · Cited by 2
Somatic embryogenesis and long term conservation of cocoa (Theobroma cacao L.) germplasm
Gámez, D. A. J. (2012) · Cited by 2
Writing Standard Operating Procedures (SOPs) for Cryostorage Protocols: Using Shoot Meristem Cryopreservation as an Example
Harding, K., & Benson, E. E. (2014) · Methods in molecular biology · doi:10.1007/978-1-4939-2193-5_22 · Cited by 1
Study of Osmotic Behavior of Astasia longa and Dunaliella salina Cells as an Important Stage in Developing Their Cryopreservation Protocol
Chernobai, N., Kadnikova, N., Vozovyk, K., et al. (2021) · doi:10.3390/iecps2021-11939 · Cited by 1
“The traditional methods of longterm storage of eukaryotic microalgae and protist, unfortunately, cannot fully meet the above requirements [3,4].”
Method
Unit operations for extraction and purification of biological products
Castro, F., da Silva, N. R., Silvério, S. C., et al. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-91167-2.00005-8 · Cited by 1
Toxicity and toxicodynamics of cryoprotectant used in pharmaceutical products
Pingale, P., Rajput, A., Chavan, Y. S., Patil, R., Singh, A. D., & Tekade, R. K. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-15842-1.00020-x · Cited by 1
Osteoinductive accelerating potential of myxobacterial secondary metabolites
Parhizkari, H., Mohammadipanah, F., & Hodjat, M. (2024) · The Microbe · doi:10.1016/j.microb.2024.100204 · Cited by 1
Two-photon imaging of intact living plants during freezing with a flexible multiphoton tomograph
Breunig, H. G., & König, K. (2015) · Laser Physics Letters · doi:10.1088/1612-2011/12/2/025601 · Cited by 1
Cryopreservation of Tobacco BY-2 Suspension Cell Cultures Using an Encapsulation — Simple Prefreezing Method
Kobayashi, T., Niino, T., & Kobayashi, M. (2006) · Springer eBooks · doi:10.1007/3-540-32674-x_21 · Cited by 1
Powders containing microorganisms and enzymes
Ghandi, A., Adhikari, B., & Powell, I. B. (2024) · Elsevier eBooks · doi:10.1016/b978-0-323-98820-9.00008-9 · Cited by 1
Antibacterial and cytotoxic evaluation of sequential extract of Moringa oleifera leaves
Naeem, U. B., Iftikhar, W., Rafiq, M., et al. (2022) · Bionatura · doi:10.21931/rb/2022.07.01.15 · Cited by 1
Heat and Mass Transfer Models and Measurements for Low-Temperature Storage of Biological Systems
Shaik, S. M., & Devireddy, R. V. (2017) · Springer eBooks · doi:10.1007/978-3-319-32003-8_73-1 · Cited by 1
Novel Cryopreservation Strategies for Cell‐Therapies and Pre‐Clinical Research
Malpique, R. (2010) · Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) · Cited by 1
Raman scattering evidence of hydrohalite formation on frozen yeast cellsPreprint
Okotrub, K. A., & Surovtsev, N. V. (2012) · arXiv (Cornell University) · doi:10.48550/arxiv.1208.5586 · Cited by 1
“While most cryopreservation protocols have been developed empirically [9], our understanding of physical processes underneath of the cryoprocesses is still obscure.”
Background
Freezing technology
Gökoğlu, N., & Yerlikaya, P. (2015) · doi:10.1002/9781118512210.ch7 · Cited by 1
Mathematical modelling for development, scale-up and optimization of a process of lyophilisation
Duarte, M. L. A. (2018) · Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) · Cited by 1
Culture Collections
Smith, D. I. (2014) · Encyclopedia of Food Microbiology · doi:10.1016/b978-0-12-384730-0.00079-3 · Cited by 1
Smart Macroporous Structures for the Purification of Viral Particles
Sousa, M. (2014) · New University of Lisbon's Repository (New University of Lisbon)
Virus-enabled synthesis and 2D/3D assembly of nanowire networks
Liau, F. (2013) · DSpace@MIT (Massachusetts Institute of Technology)
Alternative Methods for Long Term Storage of Skeletal Muscle Tissue
Hempel, B. (2014) · UKnowledge (University of Kentucky)
Growth Kinetic Study on Lyophilized and Cryopreserved Pleurotus sajor-caju Spawn
Shayuti, S. A. M., Chong, S. F., Zakaria, Z., et al. (2015) · Applied Mechanics and Materials · doi:10.4028/www.scientific.net/amm.754-755.1076
Avaliação das características seminais e criopreservação do sêmen de Pseudoplatystoma corruscans (Siluriforme pimelodidae)
Velarde, J. M. C. (2013) · Americanae (AECID Library)
Viabilidad de cepas de hongos Basidiomycetes bajo diferentes técnicas de conservación: procesos de crioconservación vs. liofilización
Palacio, A., Gutiérrez, Y., Rojas, D., et al. (2017) · Vitae (Universidad de Antioquia)
A comparative analysis of preservation of functional food cultures by freeze-drying, liquid-drying and freezing methods
Tan, G. H., & Mustapha, N. (2014) · Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia)
Derivation of Stem Cells from in vivo Produced Goat Embryos
Lam, L. (2013) · Libraries and Cultural Resources (University of Calgary) · doi:10.11575/prism/25560
Investigation into the Influence of Temperature on the Cryopreservation of Mesenchymal Stem Cells
Norkus, M. (2012) · ARAN (University of Galway Research Repository) (Ollscoil na Gaillimhe – University of Galway) · doi:10.13025/16096
Modification of a whole-cell biosensor to detect toxins
Zealand, N. (2012)
A new method for long-term storage of titred microbial standard solutions suitable for microbiologic quality control activities of pharmaceutical companies
Chiellini, C., Mocali, S., Fani, R., et al. (2016) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2016.05.016
IDENTIFICACIÓN DE CEPAS NATIVAS CON POTENCIAL PARA OBTENCIÓN DE POLIHIDROXIALCANOATOS - (PHAs) EN LODOS ACTIVADOS IDENTIFICATION OF STRAINS NATIVE WITH POTENTIAL TO OBTAIN POLYHYDROXYALKANOATES-(PHAS) IN ACTIVATED SLUGDES
Lorena, A. C., Arroyave, R., Cardona, B., et al. (2013)
Efeitos de baixas doses de radiação gama no crescimento e na síntese lipídica de Yarrowia lipolytica
Delabio, A. D. S. (2016) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/d.64.2016.tde-23032016-151832
Agrobiodiversity genetic resources conservation for the development of sustainable production systems Conservación de recursos genéticos de la agrobiodiversidad como apoyo al desarrollo de sistemas de producción sostenibles
Arias, M. L., Inés, C., & Cano, M. (2009)
Vitrification for IVF
Dorfmann, A. D. (2017) · Cambridge University Press eBooks · doi:10.1017/9781316569238.011
Caracterización molecular y criopreservación de hongos y bacterias asociados a la rizósfera de especies vegetales acumuladoras de metales pesados en suelos contaminados por minería.
Ecuador, D. D. C. B. U. T. P. D. L., Ochoa, J. F., Sánchez‐Rodríguez, A., et al. (2017) · Bionatura · doi:10.21931/rb/2017.02.02.3
Aislamiento, caracterización y estudios de actividad de bacteriófagos líticos sobre Enterococcus spp. en modelos in vivo e in vitro
Ramírez, B. A. (2016) · doi:10.35537/10915/57371
Comprehensive evaluation of different cryopreservation methods used for the successful storage of human Wharton's Jelly tissue
Mallis, P., Georgiou, E., Michalopoulos, Ε., et al. (2018) · Biomedical Research and Clinical Practice · doi:10.15761/brcp.1000157
Effect of the Thermodynamic and Physical State of the Freeze-Concentrate on Protein Stability
Jena, S. (2017) · University of Minnesota Digital Conservancy (University of Minnesota)
Brusella abortus S-19 aşısının liyofilizasyon öncesi farklı liyoprotektanlar ile kritik formülasyon sıcaklıklarının araştırılması
KARAGÜL, M. S., & EROĞLU, B. (2019) · Etlik Veteriner Mikrobiyoloji Dergisi · doi:10.35864/evmd.560109
Numerical Evaluation of Residual Water Content after Freezing during the Lyophilization of Platelets
Zhang, S., Xie, R., Xu, M., et al. (2020) · Fluid dynamics & materials processing · doi:10.32604/fdmp.2020.010352
Exploración de un transportador de NAD+ y/o sus precursores en Leishmania
Silva, V., & Eliana, S. (2021)
DETERMINACIÓN DE LA PRODUCCIÓN DE EXTRAPOLISACÁRIDO DE BACTERIAS PROVENIENTES DE RESIDUOS OLIVÍCOLAS
Robledo, F. A., Soloaga, M. A., Cordoba, P. A., et al. (2021) · Editora Artemis eBooks · doi:10.37572/edart_21122150712

Authors: Day, J., & Stacey, G.
Affiliations: National Institute for Biological Standards and Control (GB)
Venue: Methods in Molecular Biology
Publisher: Humana Press
Published: 2007
ISSN: 1064-3745 (ISSN-L)
1940-6029
DOI: 10.1007/978-1-59745-362-2
OpenAlex: W1506233354
Cited by: 318
References: 0
Citations by year: 2026: 2 · 2025: 5 · 2024: 11 · 2023: 15 · 2022: 15 · 2021: 21 · 2020: 16 · 2019: 19 · 2018: 21 · 2017: 25 · 2016: 14 · 2015: 17 · 2014: 16 · 2013: 23 · 2012: 27
FWCI: 13.41
Type: book
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Methods in molecular biology (publishedVersion)
DIAL (Catholic University of Leuven) (submittedVersion)
Funders: NHS Blood and Transplant
University of Bedfordshire
Cardiff University
Directorate for Biological Sciences
Biotechnology and Biological Sciences Research Council
Universitätsklinikum Hamburg-Eppendorf
Topics: Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
Keywords: cryopreservation, freeze-drying, ex situ conservation, biological resource centers, protein preservation, long-term preservation

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Cryopreservation and Freeze-Drying Protocols

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cited by

Related

Metadata

Record

About these checks