Infectivity of influenza B virus in cultured human muscle
Abstract
Severe muscle symptoms, particularly in children, are frequently associated with influenza B virus infection. In this study we examined the effects of influenza B virus (Lee Strain) on cultured human muscle by light and electron microscopy (EM), immunofluorescence, hemadsorption and plaque assays. Muscle injury was also evaluated by the appearance of muscle-specific creatine kinase (CK) in the culture medium. By fluorescence immunocytochemistry viral antigen was demonstrated in muscle cell nuclei within 3 h postinoculation (p.i.) and in the cytoplasm at 6 h p.i. Membrane-associated viral antigen was seen at 16 h p.i., at which time budding influenza virus-like particles could be demonstrated by EM, both in myoblasts and multinucleated myotubes. At 16 h all cells were hemadsorption positive. Plaque assays showed peak virus production at 48 h (p.i.), at which time cytopathic effects (cell retraction, pycnosis and cytoplasmic vacuolization) were prominent and some cells detached from the substratum. Leakage of muscle-specific CK isozyme into the culture medium could be demonstrated as early as 6 h p.i. with peak enzyme activity around 40-48 h p.i. Cytopathic changes and virus production were observed both in myoblasts and myotubes indicating that both cell types are susceptible.
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Influenza B virus can replicate in regenerating human muscle cell cultures.
“Influenza B virus can replicate in regenerating human muscle cell cultures”
From Myocardial Injury and Bacterial Pneumonia Contribute to the Pathogenesis of Fatal Influenza B Virus Infection (2012) · cites this paper as [45]
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- Authors
- Servidei, S., Miranda, A. F., & Gamboa, E. T.
- Affiliations
- Columbia University (US)
- ORCID
- Servidei, S.
- Venue
- Acta Neuropathologica
- Published
- 1987
- Volume
- 73
- Issue
- 1
- Pages
- 67-76
- ISSN
- 0001-6322 (ISSN-L)
- 1432-0533
- OpenAlex
- W1981144389
- Cited by
- 24
- References
- 35
- Citations by year
- 2025: 1 · 2024: 2 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 1 · 2016: 1 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Acta Neuropathologica (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (NS-11766, NS-18446)
- Topics
- Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
- Keywords
- influenza B, Lee strain, muscle infection, myoblasts, myotubes, cytopathic effect, viral antigen localization, viral budding, hemoadsorption, plaque formation, muscle damage
- MeSH terms
- Creatine Kinase, Fluorescent Antibody Technique, Hemagglutination, Viral, Humans, Influenza, Human, Muscles, Myositis, Influenza B virus, Virus Replication, Culture Techniques
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