Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae—A review

Lees, N. D.; Skaggs, B.; Kirsch, D. R.; Bard, M.

doi:10.1007/bf02537824

Cited by 202Fungal and yeast genetics researchPlant biochemistry and biosynthesisAntifungal resistance and susceptibility

Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae—A review

Lees, N. D., Skaggs, B., Kirsch, D. R., Bard, M.·1995·Lipids

DOI 10.1007/bf02537824

Open this paper in CoChat — free account

Abstract

Research on the ergosterol biosynthetic pathway in fungi has focused on the identification of the specific sterol structure required for normal membrane structure and function and for completion of the cell cycle. The pathway and its end product are also the targets for a number of antifungal drugs. Identification of essential steps in ergo-sterol biosynthesis could provide new targets for the development of novel therapeutic agents. Nine of the eleven genes in the portion of the pathway committed exclusively to ergosterol biosynthesis have been cloned, and their essentiality for aerobic growth has been determined. The first three genes, ERG9 (squalene synthase), ERG1 (squalene epoxidase), and ERG7 (lanosterol synthase), have been cloned and found to be essential for aerobic viability since their absence would result in the cell being unable to synthesize a sterol molecule. The remaining eight genes encode enzymes which metabolize the first sterol, lanosterol, to ultimately form ergosterol. The two earliest genes, ERG11 (lanosterol demethylase) and ERG24 (C-14 reductase), have been cloned and found to be essential for aerobic growth but are suppressed by mutations in the C-5 desaturase (ERG3) gene and fen1 and fen2 mutations, respectively. The remaining cloned genes, ERG6 (C-24 methylase), ERG2 (D8AE7 isomerase), ERG3 (C-5 desaturase), and ERG4 (C-24(28) reductase), have been found to be nonessential. The remaining genes not yet cloned are the C-4 demethylase and the C-22 des…

Integrity signals

Registry

Crossref · 10.1007/bf02537824

Cites

57 of 57 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

N. crassa deletion mutants Δerg2, Δerg3, Δerg4 and Δerg5 are viable.
“All the mentioned gene deletion mutants are viable (Figure 4A), similarly to that described in S. cerevisiae (Lees et al., 1995).”
From Abnormal Ergosterol Biosynthesis Activates Transcriptional Responses to Antifungal Azoles (2018) · cites this paper as Lees et al., 1995

Regulation of the mevalonate pathway
Goldstein, J. L., & Brown, M. S. (1990) · Nature · doi:10.1038/343425a0 · Cited by 5,394 · see citing works
Inactivation of Antibiotics and the Dissemination of Resistance Genes
DAVIES, J. E. (1994) · Science · doi:10.1126/science.8153624 · Cited by 1,729 · see citing works
Essential role for mevalonate synthesis in DNA replication
Quesney-Huneeus, V., Wiley, M. H., & Siperstein, M. D. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.10.5056 · Cited by 297 · see citing works
In vitro and in vivo effects of the antimycotic drug ketoconazole on sterol synthesis
den Bossche, H. V., Willemsens, G., Cools, W., Cornelissen, F. W., Lauwers, W., & Cutsem, J. M. V. (1980) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.17.6.922 · Cited by 277 · see citing works
The yeast gene ERG6 is required for normal membrane function but is not essential for biosynthesis of the cell-cycle-sparking sterol.
Gaber, R. F., Copple, D. M., Kennedy, B. K., Vidal, M., & Bard, M. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.8.3447 · Cited by 257 · see citing works
Self-citation
Multiple functions for sterols in Saccharomyces cerevisiae
Rodriguez, R. J., Low, C., Bottema, C. D. K., & Parks, L. W. (1985) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(85)90057-8 · Cited by 253 · see citing works
Isolation of an Arabidopsis thaliana gene encoding cycloartenol synthase by functional expression in a yeast mutant lacking lanosterol synthase by the use of a chromatographic screen.
Corey, E. J., Matsuda, S., & Bartel, B. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.24.11628 · Cited by 240 · see citing works
The lamin B receptor of the nuclear envelope inner membrane: a polytopic protein with eight potential transmembrane domains.
Worman, H. J., Evans, C. D., & Blobel, G. (1990) · The Journal of Cell Biology · doi:10.1083/jcb.111.4.1535 · Cited by 236 · see citing works
Human Mycoses: Drugs and Targets for Emerging Pathogens
Georgopapadakou, N. H., & Walsh, T. J. (1994) · Science · doi:10.1126/science.8153622 · Cited by 234 · see citing works
Reviving the Antibiotic Miracle?
Travis, J. W. (1994) · Science · doi:10.1126/science.8153615 · Cited by 201 · see citing works
Defective sterol C5-6 desaturation and azole resistance: A new hypothesis for the mode of action of azole antifungals
Watson, P. F., Rose, M. E., Ellis, S. W., England, H., & Kelly, S. L. (1989) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(89)91792-0 · Cited by 197 · see citing works
The dual role of mevalonate in the cell cycle.
Quesney-Huneeus, V., Galick, H. A., Siperstein, M. D., Erickson, S. K., Spencer, T. A., & Nelson, J. A. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)33268-x · Cited by 195 · see citing works
Primary Structure of the P450 Lanosterol Demethylase Gene from Saccharomyces cerevisiae
Kalb, V. F., Woods, C. W., Turi, T. G., Dey, C. R., Sutter, T. R., & LOPER, J. C. (1987) · DNA · doi:10.1089/dna.1987.6.529 · Cited by 176 · see citing works
Genetic characterization of pathogenic Saccharomyces cerevisiae isolates.
McCusker, J. H., Clemons, K. V., Stevens, D. A., & Davis, R. W. (1994) · Genetics · doi:10.1093/genetics/136.4.1261 · Cited by 171 · see citing works
Molecular cloning and characterization of the yeast gene for squalene synthetase.
Jennings, S., Tsay, Y. H., Fisch, T. M., & Robinson, G. W. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.14.6038 · Cited by 162 · see citing works
Conservation between Human and Fungal Squalene Synthetases: Similarities in Structure, Function, and Regulation
Robinson, G. W., Tsay, Y. H., Kienzle, B. K., Smith-Monroy, C., & Bishop, R. W. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.5.2706 · Cited by 158 · see citing works
Cholesterol in mycoplasma membranes. Composition, ultrastructure and biological properties of membranes from Mycoplasma mycoides var. capri cells adapted to grow with low cholesterol concentrations
Rottem, S., Yashouv, J., Neʼeman, Z., & Razin, S. (1973) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(73)90158-2 · Cited by 157 · see citing works
Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase
Arthington, B. A., Bennett, L. G., Skatrud, P. L., Guynn, C. J., Barbuch, R. J., UIbright, C. E., & Bard, M. (1991) · Gene · doi:10.1016/0378-1119(91)90535-j · Cited by 149 · see citing works
Self-citation
Inhibition of ergosterol biosynthesis in saccharomyces cerevisiae and Ustilago maydis by tridemorph, fenpropimorph and fenpropidin
Baloch, R. I., MERCER, E. I., Wiggins, T. E., et al. (1984) · Phytochemistry · doi:10.1016/s0031-9422(00)80523-3 · Cited by 127 · see citing works
In vivo effects of fenpropimorph on the yeast Saccharomyces cerevisiae and determination of the molecular basis of the antifungal property
Marcireau, C., Guilloton, M., & Karst, F. (1990) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.34.6.989 · Cited by 125 · see citing works
A protein kinase antigenically related to pp60v-src possibly involved in yeast cell cycle control: positive in vivo regulation by sterol.
Dahl, C., Biemann, H. P., & Dahl, J. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.12.4012 · Cited by 122 · see citing works
Sterol synthesis and viability oferg11 (cytochrome P450 lanosterol demethylase) mutations inSaccharomyces cerevisiae andCandida albicans
Bard, M., Lees, N. D., Turi, T. G., Craft, D., Cofrin, L., Barbuch, R. J., Koegel, C., & Loper, J. C. (1993) · Lipids · doi:10.1007/bf02537115 · Cited by 121 · see citing works
Self-citation
The gene encoding squalene epoxidase from Saccharomyces cerevisiae: cloning and characterization
Jandrositz, A., Turnowsky, F., & Högenauer, G. (1991) · Gene · doi:10.1016/0378-1119(91)90310-8 · Cited by 119 · see citing works
Structural and physiological features of sterols necessary to satisfy bulk membrane and sparking requirements in yeast sterol auxotrophs
Rodriguez, R. J., & Parks, L. W. (1983) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(83)90099-1 · Cited by 111 · see citing works
Molecular cloning, characterization, and overexpression of ERG7, the Saccharomyces cerevisiae gene encoding lanosterol synthase.
Corey, E. J., Matsuda, S., & Bartel, B. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.6.2211 · Cited by 110 · see citing works
Biology of Cholesterol
Yèagle, P. L. (1988) · Medical Entomology and Zoology · Cited by 92 · see citing works
Stereochemical specificity for sterols in Saccharomyces cerevisiae.
Pinto, W. J., & Nes, W. R. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32647-4 · Cited by 83 · see citing works
Differences in crystal violet uptake and cation-induced death among yeast sterol mutants
Bard, M., Lees, N. D., Burrows, L. S., & Kleinhans, F. W. (1978) · Journal of Bacteriology · doi:10.1128/jb.135.3.1146-1148.1978 · Cited by 83 · see citing works
Self-citation
Isolation of a cytochrome P-450 structural gene from Saccharomyces cerevisiae
Kalb, V. F., Loper, J. C., Dey, C. R., Woods, C. W., & Sutter, T. R. (1986) · Gene · doi:10.1016/0378-1119(86)90021-1 · Cited by 81 · see citing works
Total enzymic synthesis of cholesterol from lanosterol. Cytochrome b5-dependence of 4-methyl sterol oxidase.
Fukushima, H., Grinstead, G. F., & Gaylor, J. L. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)69327-0 · Cited by 81 · see citing works
Cloning and disruption of the yeast C‐8 sterol isomerase gene
Ashman, W. H., Barbuch, R. J., Ulbright, C. E., Jarrett, H. W., & Bard, M. (1991) · Lipids · doi:10.1007/bf02536427 · Cited by 79 · see citing works
Self-citation
The Yeast Gene ERG6 Is Required for Normal Membrane Function but Is Not Essential for Biosynthesis of the Cell-Cycle-Sparking Sterol
Gaber, R. F., Copple, D. M., Kennedy, B. K., Vidal, M., & Bard, M. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.8.3447-3456.1989 · Cited by 78 · see citing works
Self-citation
Yeast mutants blocked in removing the methyl group of lanosterol at C-14. Separation of sterols by high-pressure liquid chromatography
Trocha, P. J., Jasne, S. J., & Sprinson, D. B. (1977) · Biochemistry · doi:10.1021/bi00640a029 · Cited by 69 · see citing works
The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway
LAI, M. H., Bard, M., Pierson, C. A., F.Alexander, J., Goebl, M. G., Carter, G. T., & Kirsch, D. R. (1994) · Gene · doi:10.1016/0378-1119(94)90728-5 · Cited by 69 · see citing works
Self-citation
Cloning, Sequencing, and Disruption of the Gene Encoding Sterol C-14 Reductase in Saccharomyces cerevisiae
Lorenz, R. T., & Parks, L. W. (1992) · DNA and Cell Biology · doi:10.1089/dna.1992.11.685 · Cited by 67 · see citing works
Strategies in prevention of invasive pulmonary aspergillosis in immunosuppressed or neutropenic patients
Beyer, J., Schwartz, S., Heinemann, V., & Siegert, W. (1994) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.38.5.911 · Cited by 66 · see citing works
Construction and growth properties of a yeast strain defective in sterol 14-reductase
Marcireau, C., Guyonnet, D., & Karst, F. (1992) · Current Genetics · doi:10.1007/bf00317919 · Cited by 62 · see citing works
Isolation and primary structure of the ERG9 gene of Saccharomyces cerevisiae encoding squalene synthetase
Fégueur, M., Richard, L., Charles, A., et al. (1991) · Current Genetics · doi:10.1007/bf00317063 · Cited by 59 · see citing works
ESR determination of membrane order parameter in yeast sterol mutants
Lees, N. D., Bard, M., Kemple, M. D., Haak, R. A., & Kleinhans, F. W. (1979) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(79)90302-x · Cited by 58 · see citing works
Self-citation
Cloning and characterization of the 2,3-oxidosqualene cyclase-coding gene of Candida albicans
Kelly, R., Miller, S. M., LAI, M. H., & Kirsch, D. R. (1990) · Gene · doi:10.1016/0378-1119(90)90299-7 · Cited by 58 · see citing works
Self-citation
Stereochemically distinct roles for sterol in Saccharomycescerevisiae
Pinto, W. J., Lozano, R., Sekula, B. C., & Nes, W. R. (1983) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(83)91795-3 · Cited by 56 · see citing works
Structural discrimination in the sparking function of sterols in the yeast Saccharomyces cerevisiae
Lorenz, R. T., Casey, W., & Parks, L. W. (1989) · Journal of Bacteriology · doi:10.1128/jb.171.11.6169-6173.1989 · Cited by 52 · see citing works
Requirement for a second sterol biosynthetic mutation for viability of a sterol C-14 demethylation defect in Saccharomyces cerevisiae
Taylor, F. R., Rodriguez, R. J., & Parks, L. W. (1983) · Journal of Bacteriology · doi:10.1128/jb.155.1.64-68.1983 · Cited by 51 · see citing works
XIII . Yeast sequencing reports. SED6 is identical to ERG6, and encodes a putative methyltransferase required for ergosterol synthesis
Hardwick, K., & Pelham, H. R. B. (1994) · Yeast · doi:10.1002/yea.320100213 · Cited by 45 · see citing works
ESR determinations of membrane permeability in a yeast sterol mutant
Kleinhans, F. W., Lees, N. D., Bard, M., Haak, R. A., & Woods, R. A. (1979) · Chemistry and Physics of Lipids · doi:10.1016/0009-3084(79)90042-2 · Cited by 45 · see citing works
Self-citation
The Effects of Varied Energy Source and Detergent on the Growth of Sterol Mutants of Saccharomyces cerevisiae
Lees, N. D., LOFTON, S. L., Woods, R. A., et al. (1980) · Microbiology · doi:10.1099/00221287-118-1-209 · Cited by 45 · see citing works
Self-citation
Conservation between Human and Fungal Squalene Synthetases: Similarities in Structure, Function, and Regulation
Robinson, G. W., Tsay, Y. H., Kienzle, B. K., et al. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.5.2706-2717.1993 · Cited by 37 · see citing works
Azole susceptibility and hyphal formation in a cytochrome P-450-deficient mutant of Candida albicans
Lees, N. D., Broughton, M. C., Sanglard, D., & Bard, M. (1990) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.34.5.831 · Cited by 35 · see citing works
Self-citation
Accumulation of 14-methyl sterols and defective hyphal growth inCandida albicans
Shimokawa, O., Kato, Y., & Nakayama, H. (1986) · Medical Mycology · doi:10.1080/02681218680000481 · Cited by 34 · see citing works
Fission yeast sts1+ gene encodes a protein similar to the chicken lamin B receptor and is implicated in pleiotropic drug-sensitivity, divalent cation-sensitivity, and osmoregulation.
Shimanuki, M., Goebl, M. G., Yanagida, M., & Toda, T. (1992) · Molecular Biology of the Cell · doi:10.1091/mbc.3.3.263 · Cited by 28 · see citing works
Nucleotide sequence of the gene encoding yeast C-8 sterol isomerase
Arthington, B. A., Hoskins, J., Skatrud, P. L., et al. (1991) · Gene · doi:10.1016/0378-1119(91)90314-2 · Cited by 26 · see citing works
Inhibitors of 2,3-oxidosqualene lanosterol-cyclase as potential antifungal agents
Jolidon, S., Polak, A., Guerry, P., et al. (1990) · Biochemical Society Transactions · doi:10.1042/bst0180047 · Cited by 25 · see citing works
Accumulation of 14α-methylergosta-8,24(28)-dien-3β,6α-diol in 14α-demethylation mutants of Candida albicans: genetic evidence for the involvement of 5-desaturase
Shimokawa, O., Kato, Y., Kawano, K., et al. (1989) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(89)90092-1 · Cited by 23 · see citing works
VII . Yeast sequencing reports. The YGL022 gene encodes a putative transport protein
Chen, W., Capieaux, E., Balzi, E., & Goffeau, A. (1991) · Yeast · doi:10.1002/yea.320070313 · Cited by 21 · see citing works
The polyene macrolide antibiotics
Kerridge, D. (1979) · Postgraduate Medical Journal · doi:10.1136/pgmj.55.647.653 · Cited by 14 · see citing works
Membrane fluidity alterations in a cytochrome P-450-deficient mutant of candida albicans
Lees, N. D., Kleinhans, F. W., Broughton, M. C., Pennington, D. E., Ricker, V. A., & Bard, M. (1989) · Steroids · doi:10.1016/0039-128x(89)90032-9 · Cited by 10 · see citing works
Self-citation
Predicting the Weather
Sheppard, P. A. (1973) · Nature · doi:10.1038/243425a0 · Cited by 8 · see citing works

Flagged first, then by citations

Sorted by citations

Clinical, Cellular, and Molecular Factors That Contribute to Antifungal Drug Resistance
White, T. C., Marr, K. A., & Bowden, R. A. (1998) · Clinical Microbiology Reviews · doi:10.1128/cmr.11.2.382 · Cited by 1,353 · see citing works
Upregulation of ERG Genes in Candida Species by Azoles and Other Sterol Biosynthesis Inhibitors
Henry, K. W., Nickels, J. T., & Edlind, T. D. (2000) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.44.10.2693-2700.2000 · Cited by 216 · see citing works
Role of the non‐respiratory pathways in the utilization of molecular oxygen by Saccharomyces cerevisiae
Rosenfeld, E., & Beauvoit, B. (2003) · Yeast · doi:10.1002/yea.1026 · Cited by 149 · see citing works
Molecular Mechanisms of Drug Resistance in Clinical Candida Species Isolated from Tunisian Hospitals
Eddouzi, J., Parker, J. E., Vale-Silva, L. A., Coste, A., Ischer, F., Kelly, S., Manai, M., & Sanglard, D. (2013) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00555-13 · Cited by 128 · see citing works
A Nonsense Mutation in the ERG6 Gene Leads to Reduced Susceptibility to Polyenes in a Clinical Isolate of Candida glabrata
Vandeputte, P., Tronchin, G., Larcher, G., Ernoult, E., Bergès, T., Chabasse, D., & Bouchara, J.-P. (2008) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00423-08 · Cited by 117 · see citing works
Abnormal Ergosterol Biosynthesis Activates Transcriptional Responses to Antifungal Azoles
Hu, C., Zhou, M., Wang, W., Sun, X., Yarden, O., & Li, S. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00009 · Cited by 114 · see citing works
Reduced Susceptibility to Polyenes Associated with a Missense Mutation in the ERG6 Gene in a Clinical Isolate of Candida glabrata with Pseudohyphal Growth
Vandeputte, P., Tronchin, G., Bergès, T., Hennequin, C., Chabasse, D., & Bouchara, J.-P. (2007) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01510-06 · Cited by 109 · see citing works
Transcriptional regulation of the squalene synthase gene (ERG9) in the yeast Saccharomyces cerevisiae
Kennedy, M. A., Barbuch, R., & Bard, M. (1999) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(99)00035-4 · Cited by 85 · see citing works
Self-citation
Mot3 is a transcriptional repressor of ergosterol biosynthetic genes and is required for normal vacuolar function in Saccharomyces cerevisiae
Hongay, C., Jia, N., Bard, M., & Winston, F. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf415 · Cited by 72 · see citing works
Self-citation
Sterol C-22 desaturase ERG5 mediates the sensitivity to antifungal azoles in Neurospora crassa and Fusarium verticillioides.
Sun, X., Wang, W., Wang, K., Yu, X., Liu, J., Zhou, F., Xie, B., & Li, S. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00127 · Cited by 37 · see citing works

A novel sequence element is involved in the transcriptional regulation of expression of the ERG1 (squalene epoxidase) gene in Saccharomyces cerevisiae
Leber, R., Zenz, R., Schröttner, K., Fuchsbichler, S., Pühringer, B., & Turnowsky, F. (2001) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.2001.01940.x · Cited by 56 · see citing works
Enhanced squalene production by modulation of pathways consuming squalene and its precursor
Tominaga, M., Miyazaki, K., Hataya, S., Mitsui, Y., Kuroda, S., Kondo, A., & Ishii, J. (2022) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2022.04.004 · Cited by 17 · see citing works
Terbinafine: Mode of action and properties of the squalene epoxidase inhibition
Ryder, N. S. (1992) · British Journal of Dermatology · doi:10.1111/j.1365-2133.1992.tb00001.x · Cited by 440 · see citing works
Effect of Fluconazole and Terbinafine on the Activities of Lanosterol 14 α-Demethylase and Squalene Epoxidase in Ergosterol Biosynthetic Pathway
(2014) · Egyptian Journal of Botany · doi:10.21608/ejbo.2014.490 · Cited by 1 · see citing works
Combined overexpression of genes of the ergosterol biosynthetic pathway leads to accumulation of sterols in
Veen, M., Ståhl, U., & LANG, C. (2003) · FEMS Yeast Research · doi:10.1016/s1567-1356(03)00126-0 · Cited by 134 · see citing works

Authors: Lees, N. D., Skaggs, B., Kirsch, D. R., & Bard, M.
Affiliations: Indiana University – Purdue University Indianapolis (US)
Agricultural Research Division American Cyanamid Princeton New Jersey 08543‐0400
Venue: Lipids
Publisher: Wiley
Published: 1995-03
Volume: 30
Issue: 3
Pages: 221-226
ISSN: 0024-4201 (ISSN-L)
1558-9307
DOI: 10.1007/bf02537824
OpenAlex: W2028967650
Cited by: 202
References: 57
Citations by year: 2026: 8 · 2025: 4 · 2024: 6 · 2023: 1 · 2022: 4 · 2021: 4 · 2020: 6 · 2019: 6 · 2018: 5 · 2017: 8 · 2016: 2 · 2015: 2 · 2014: 8 · 2013: 6 · 2012: 12
FWCI: 0.79
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Lipids (publishedVersion)
PubMed (publishedVersion)
Funders: Eli Lilly and Company
Indiana University-Purdue University Indianapolis
National Institute of General Medical Sciences (IR15GM45959-01)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Keywords: ergosterol biosynthesis, late genes, Saccharomyces cerevisiae, gene cloning, ERG1, ERG11, ERG3, ERG6, ERG2
MeSH terms: Cloning, Molecular, Ergosterol, Genes, Fungal, Lanosterol, Saccharomyces cerevisiae, Squalene, Time Factors

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae—A review

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Cites

Cited by

Related

Record

About these checks