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Cited by 674

The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.

Analysis of Nuclear DNA content in plant cells by Flow cytometry

Dpooležel, J., Binarová, Pavla, Lcretti, S.·1989·Biologia Plantarum

Abstract

Flow cytometry was used to analyse the DNA content of nuclei isolated from intact plant tissues and from callus and cell suspension cultures in vitro . Cell nuclei were isolated either mechanically (chopping, syringing) or by a hypotonic lysis of isolated protoplasts. Although both methods gave similar results, a slight shift to lower ploidy levels was observed after protoplast isolation from intact tissues and calli. No differences were observed if the two methods were compared using cell suspension cultures. The results showed that flow cytometry is a rapid method of nuclear DNA content analysis in intact plant tissues and various in vitro cultures.

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Metadata

Authors
Dpooležel, J., Binarová, P., & Lcretti, S.
Venue
Biologia Plantarum
Published
1989-03
Volume
31
Issue
2
Pages
113-120
ISSN
  • 0006-3134 (ISSN-L)
  • 1573-8264
OpenAlex
W2091543204
Cited by
674
References
6
FWCI
1.49
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Analysis of Nuclear DNA content in plant cells by Flow cytometry. https://app.cochat.ai/papers/10.1007/bf02907241
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Dpooležel, J., Binarová, P., & Lcretti, S. (1989). Analysis of Nuclear DNA content in plant cells by Flow cytometry. Biologia Plantarum. https://doi.org/10.1007/bf02907241

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.