Somaclonal variation in oil palm (Elaeis guineensis Jacq.): The DNA methylation hypothesis
Abstract
Elaeis guineensis Jacq.) currently hampers the scaling-up of clonal plant production. In order to investigate the relationship between the "mantled" somaclonal variant and possible alterations in genomic DNA methylation rate, two complementary approaches have been used. HPLC quantification of relative amounts of 5-methyl-deoxycytidine has shown that global methylation in leaf DNA of abnormal regenerants is 0.5-2.5% lower than in their normal counterparts (20.8% vs 22%, respectively). When comparing nodular compact calli and fast growing calli, yielding respectively 5% and 100% of "mantled" plantlets, this decrease was up to 4.5% (from 23.2 to 18.7%). An alternative method, the SssI-methylase accepting assay, based on the enzymatic saturation of CG sites with methyl groups, gave convergent results. This work demonstrates that a correlation exists between DNA hypomethylation and the "mantled" somaclonal variation in oil palm.
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In palm, fast growing calli generate almost 100% mantled plantlets, while nodular compact calli generate only 5% abnormal plants.
“Jaligot et al. (2000) [116], which investigated DNA methylation levels in two different palm calli: fast growing (that generate almost 100% of ”mantled” plantlets—with abnormalities in their floral development, involving an apparent feminization of male parts in the flowers of both sexes), and nodular compact calli (that generate only 5% abnormal plants).”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as Jaligot et al. (2000) [116]
There is a positive correlation between genomic DNA hypomethylation and abnormal in vitro clones regenerated from fast growing palm calli.
“Using two different analysis methods (high-performance liquid chromatography—HPLC, and SssI-mediated reverse-dosage of CG-specific methylation) for assessing the methylation rates, the research team determined that there is a positive correlation between genomic DNA hypomethylation and abnormal in vitro clones regenerated from the fast growing calli.”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [116]
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- Authors
- Jaligot, E., Rival, A., Beulé, T., Dussert, S., & Verdeil, J.-L.
- Affiliations
- Centre de Coopération Internationale en Recherche Agronomique pour le Développement (FR)
- Venue
- Plant Cell Reports
- Published
- 2000-06-13
- Volume
- 19
- Issue
- 7
- Pages
- 684-690
- ISSN
- 0721-7714 (ISSN-L)
- 1432-203X
- OpenAlex
- W2153999292
- Cited by
- 220
- References
- 0
- Citations by year
- 2026: 5 · 2025: 3 · 2024: 7 · 2023: 8 · 2022: 7 · 2021: 4 · 2020: 6 · 2019: 9 · 2018: 11 · 2017: 7 · 2016: 15 · 2015: 12 · 2014: 9 · 2013: 9 · 2012: 11
- FWCI
- 2.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Plant Cell Reports (publishedVersion)
- PubMed (publishedVersion)
- Agritrop (Cirad) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- somaclonal variation, oil palm, DNA methylation, DNA hypomethylation, 5-methyldeoxycytidine, callus, mosaicism
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