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Preparation of giant vesicles by external AC electric fields. Kinetics and applications
Abstract
We suggest a method for preparation of large (∼ 15 ÷ 70 μm in diameter), unilamellar, fluctuating, isolated vesicles, allowing us to perform routine measurements of membrane bending elasticity modulus k c by image analysis of thermal fluctuations. The method is based on the effects of AC electric fields on the lipid swelling and liposome formation. We have observed a monotonous decrease of k c for egg phosphatidylcholine (EPC) vesicles from (0.66 ± 0.06) · 10−19 J for 1 day to (0.45 ± 0.05) · 10−19 J for 13 days after vesicle formation. The lipid mixture Chol/PC 37:63 mol/mol led to k c = (1.06 ± 0.15) · 10−19 J, and PE/PC 25:75 mol/mol — to (0.72 ± 0.06) · 10−19 J. — The mechanism of vesicle formation is discussed. It proved that the basic phenomenon is sequences of lateral fusions of smaller vesicles induced by the applied AC electric field.
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Metadata
- Authors
- Angelova, M. I., Soléau, S., Méléard, P., Faucon, F., & Bothorel, P.
- Venue
- Progress in Colloid & Polymer Science
- Publisher
- Steinkopff
- Published
- 2007-12-10
- Pages
- 127-131
- OpenAlex
- W2237164154
- Cited by
- 739
- References
- 0
- FWCI
- 72.28
- Type
- book chapter
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
