The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Simple Procedure for Drug Susceptibility Testing of Mycobacterium tuberculosis Using a Commercial Colorimetic Assay
Abstract
The aim of this study was to evaluate a simple method using a commercial colorimetric assay (Alamar Blue Oxidation-Reduction Indicator; Accumed, USA) in a microtiter format for testing the susceptibility of 94 strains of Mycobacterium tuberculosis to isoniazid, rifampicin, ethambutol and streptomycin. The method makes use of one critical concentration of each drug, and the results are available within 8-10 days. Overall, 97.1% agreement with the proportion method was obtained. Full agreement was obtained for isoniazid and rifampicin. The method is simple to perform, permits visual reading of results, and is practicable for laboratories with limited resources.
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“A rapid and inexpensive colorimetric method based on the oxidation-reduction indicators Alamar blue and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] have been successfully used for determining MICs of first-line drugs in DST of Mycobacterium tuberculosis (1, 9, 20, 31, 38, 49).”
From Resazurin Microtiter Assay Plate Testing of Mycobacterium tuberculosis Susceptibilities to Second-Line Drugs: Rapid, Simple, and Inexpensive Method (2003) · cites this paper as [38]
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Cites
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A NEW FLUOROMETRIC ASSAY FOR CYTOTOXICITY MEASUREMENTS IN-VITRO
PAGE, B., PAGE, M., & NOEL, C. (1993) · International Journal of Oncology · doi:10.3892/ijo.3.3.473 · Cited by 583
Roberts, G. D., Goodman, N. L., Heifets, L., Larsh, H. W., Lindner, T. H., McClatchy, J. K., McGinnis, M. R., Siddiqi, S. H., & Wright, P. (1983) · Journal of Clinical Microbiology · doi:10.1128/jcm.18.3.689-696.1983 · Cited by 309
Colorimetric method for determining MICs of antimicrobial agents for Mycobacterium tuberculosis
Yajko, D. M., Madej, J. J., Lancaster, M. V., Sanders, C. A., Cawthon, V. L., Gee, B., Babst, A., & Hadley, W. K. (1995) · Journal of Clinical Microbiology · doi:10.1128/jcm.33.9.2324-2327.1995 · Cited by 209
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Cited by
Palomino, J.-C., Martin, A., Camacho, M., Guerra, H., Swings, J., & Portaels, F. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.8.2720-2722.2002 · Cited by 1,229
Self-citationMartin, A., Camacho, M., Portaels, F., & Palomino, J. C. (2003) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.47.11.3616-3619.2003 · Cited by 306
Self-citation
Metadata
- Authors
- Palomino, J. C., & Portaels, F.
- Affiliations
- Instituut voor Tropische Geneeskunde (BE)
- ORCID
- Palomino, J. C.
- Venue
- European Journal of Clinical Microbiology and Infectious Diseases
- Published
- 1999-06-11
- Volume
- 18
- Issue
- 5
- Pages
- 380-383
- ISSN
- 0934-9723 (ISSN-L)
- OpenAlex
- W2162228435
- Cited by
- 57
- References
- 19
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2018: 2 · 2017: 3 · 2015: 5 · 2013: 2 · 2012: 3
- FWCI
- 1.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- European Journal of Clinical Microbiology & Infectious Diseases (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Action Damien
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Keywords
- Mycobacterium tuberculosis, drug susceptibility testing, colorimetric assay, Alamar Blue, microplates, isoniazid, rifampicin, ethambutol, streptomycin, proportion method, low-resource laboratories
- MeSH terms
- Antibiotics, Antitubercular, Colorimetry, Evaluation Studies as Topic, Microbial Sensitivity Tests, Mycobacterium tuberculosis, Sensitivity and Specificity
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- CoChat. (2026). Citation check: Simple Procedure for Drug Susceptibility Testing of Mycobacterium tuberculosis Using a Commercial Colorimetic Assay. https://app.cochat.ai/papers/10.1007/pl00015025
- The paper
- Palomino, J. C., & Portaels, F. (1999). Simple Procedure for Drug Susceptibility Testing of Mycobacterium tuberculosis Using a Commercial Colorimetic Assay. European Journal of Clinical Microbiology and Infectious Diseases. https://doi.org/10.1007/pl00015025
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
