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MicroRNA-128 inhibits glioma cells proliferation by targeting transcription factor E2F3a

Zhang, Yu, Chao, Tengfei, Li, Ran, Liu, Wei, Chen, Yang, Yan, Xingqi, Gong, Yanhua, Yin, Bin, Liu, Wei, Qiang, Boqing, Zhao, Jizhong, Yuan, Jiangang, Peng, Xiaozhong·2009·Journal of Molecular Medicine
DOI 10.1007/s00109-008-0403-6
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Abstract

MicroRNAs are approximately 21nt single-stranded RNAs and function as regulators of gene expression. Previous studies have shown that microRNAs play crucial roles in tumorigenesis by targeting the mRNAs of oncogenes or tumor suppressors. Here we show that brain-enriched miR-128 is down-regulated in glioma tissues and cell lines when compared to normal brain tissues. Overexpression of miR-128 in glioma cells inhibited cell proliferation. A bioinformatics search revealed a conserved target site within the 3'untranslated region (UTR) of E2F3a, a transcription factor that regulates cell cycle progression. The protein levels of E2F3a in gliomas and normal brain tissues were negatively correlated to the expression levels of miR-128 in these tissues. Overexpression of miR-128 suppressed a luciferase-reporter containing the E2F3a-3'UTR and reduced the level of E2F3a protein in T98G cells. Moreover, knocking down of E2F3a had similar effect as overexpression of miR-128, and overexpression of E2F3a can partly rescue the proliferation inhibition caused by miR-128. Taken together, our study demonstrates that miR-128 can inhibit proliferation of glioma cells through one of its targets, E2F3a.

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Crossref · 10.1007/s00109-008-0403-6

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  • The growth and viability of both A172 and U373 cells was not significantly affected by the overexpression of miR-128.

    “Interestingly, the growth and viability of both A172 and U373 cells was not significantly affected by the overexpression of miR-128 (Fig. 3B, S5), contrary to previous studies using other glioma cell types that may retain more stem-cell like characteristics [14], [41].”

    From Reduced Expression of Brain-Enriched microRNAs in Glioblastomas Permits Targeted Regulation of a Cell Death Gene (2011) · cites this paper as [41]

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Research works printed but not linked: 26

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Metadata

Authors
Zhang, Y., Chao, T., Li, R., Liu, W., Chen, Y., Yan, X., Gong, Y., Yin, B., Liu, W., Qiang, B., Zhao, J., Yuan, J., & Peng, X.
Venue
Journal of Molecular Medicine
Publisher
Springer Science and Business Media LLC
Published
2008-09-23
Volume
87
Issue
1
Pages
43-51
ISSN
  • 0946-2716
  • 1432-1440
DOI
10.1007/s00109-008-0403-6
Cited by
218
References
0
Language
en
Licence
www.springer.com/tdm (tdm)

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