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Exosome-like vesicles released from lipid-induced insulin-resistant muscles modulate gene expression and proliferation of beta recipient cells in mice
Open accessAbstract
Aims/hypothesis The crosstalk between skeletal muscle (SkM) and beta cells plays a role in diabetes aetiology. In this study, we have investigated whether SkM-released exosome-like vesicles (ELVs) can be taken up by pancreatic beta cells and can deliver functional cargoes. Methods Mice were fed for 16 weeks with standard chow diet (SCD) or with standard diet enriched with 20% palmitate (HPD) and ELVs were purified from quadriceps muscle. Fluorescent ELVs from HPD or SCD quadriceps were injected i.v. or intramuscularly (i.m.) into mice to determine their biodistributions. Micro (mi)RNA quantification in ELVs was determined using quantitative real-time RT-PCR (qRT-PCR)-based TaqMan low-density arrays. Microarray analyses were performed to determine whether standard diet ELVs (SD-ELVs) and high palmitate diet ELVs (HPD-ELVs) induced specific transcriptional signatures in MIN6B1 cells. Results In vivo, muscle ELVs were taken up by pancreas, 24 h post-injection. In vitro, both SD-ELVs and HPD-ELVs transferred proteins and miRNAs to MIN6B1 cells and modulated gene expressions whereas only HPD-ELVs induced proliferation of MIN6B1 cells and isolated islets. Bioinformatic analyses suggested that transferred HPD-ELV miRNAs may participate in these effects. To validate this, we demonstrated that miR-16, which is overexpressed in HPD-ELVs, was transferred to MIN6B1 cells and regulated Ptch1, involved in pancreas development. In vivo, islets from HPD mice showed increased size and altered expression of genes involved in development, including Ptch1, suggesting that the effect of palm oil on islet size in vivo was reproduced in vitro by treating beta cells with HPD-ELVs. Conclusions/interpretation Our data suggest that muscle ELVs might have an endocrine effect and could participate in adaptations in beta cell mass during insulin resistance.
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In the citing paper
“These data suggested for the first time that SkM-released ELVs might transmit signals to the pancreas during the development of insulin-resistance.”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Jalabert et al., 2016
In the citing paper
“ELVs collected from conditioned medium of SkM explants from DIO mice induced specific transcriptional signatures in pancreatic MIN6B1 recipient cells compared to SkM-ELVs from NCD mice.”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Jalabert et al., 2016
In the citing paper
“these data strongly suggest that SkM can release ELV in blood”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Jalabert et al., 2016
In the citing paper
“Microarray data analyses showed that the expressions of 460 mRNAs were significantly modulated including genes involved in immune responses known to be affected in pancreatic islets of diabetic patients”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Jalabert et al., 2016
In the citing paper
“suggesting again a paracrine-like action of muscle-released ELVs.”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Jalabert et al., 2016
In the citing paper
“Twenty-four hours after injection, fluorescence was detected in the left TA and in the right quadriceps too”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Jalabert et al., 2016
In the citing paper
“Intraperitoneal injections of fluorescent SkM-ELVs resulted in the fluorescent labeling of 9 organs in mice”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Jalabert et al., 2016
In the citing paper
“Additional bioinformatic analyses combined with in vitro experiments demonstrated that SkM-ELVs mediated the transfer of miRNAs from insulin-resistant muscles into MIN6B1 and affected the expression of proliferative genes such as Ptch1”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Jalabert et al., 2016
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Citations
Cites
Smyth, G. K. (2004) · Statistical Applications in Genetics and Molecular Biology · doi:10.2202/1544-6115.1027 · Cited by 11,829
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Muscles, exercise and obesity: skeletal muscle as a secretory organ
Pedersen, B. K., & Febbraio, M. A. (2012) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2012.49 · Cited by 2,909
Exosomes: Extracellular organelles important in intercellular communication
Mathivanan, S., Ji, H. L., & Simpson, R. J. (2010) · Journal of Proteomics · doi:10.1016/j.jprot.2010.06.006 · Cited by 2,581
Lötvall, J., Hill, A. F., Hochberg, F., Buzás, E. I., Di Vizio, D., Gardiner, C., Gho, Y. S., Kurochkin, I. V., Mathivanan, S., Quesenberry, P., et al. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.26913 · Cited by 2,451
Skeletal Muscle Insulin Resistance Is the Primary Defect in Type 2 Diabetes
DeFronzo, R. A., & Tripathy, D. (2009) · Diabetes Care · doi:10.2337/dc09-s302 · Cited by 2,102 · cited 1×
“Until now, the relationship between insulin resistance in SkM and decrease of insulin secretion by the pancreas remains an enigma [28].”
BackgroundInflammation as a link between obesity, metabolic syndrome and type 2 diabetes
Esser, N., Legrand-Poels, S., Piette, J., Scheen, A. J., & Paquot, N. (2014) · Diabetes Research and Clinical Practice · doi:10.1016/j.diabres.2014.04.006 · Cited by 1,981
Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs
Villarroya-Beltri, C., Gutiérrez-Vázquez, C., Sánchez-Cabo, F., Pérez-Hernández, D., Vázquez, J., Martin-Cofreces, N., Martinez-Herrera, D. J., Pascual-Montano, A., Mittelbrunn, M., & Sánchez-Madrid, F. (2013) · Nature Communications · doi:10.1038/ncomms3980 · Cited by 1,971
Wiklander, O. P. B., Nordin, J. Z., O'Loughlin, A., Gustafsson, Y., Corso, G., Mäger, I., Vader, P., Lee, Y., Sork, H., Seow, Y., et al. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.26316 · Cited by 1,730
Self-citationSystemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells
Ohno, S.-I., Takanashi, M., Sudo, K., Ueda, S., Ishikawa, A., Matsuyama, N., Fujita, K., Mizutani, T., Ohgi, T., Ochiya, T., et al. (2013) · Molecular Therapy · doi:10.1038/mt.2012.180 · Cited by 1,666
Théry, C., Boussac, M., Véron, P., Ricciardi‐Castagnoli, P., Raposo, G., Garin, J., & Amigorena, S. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.166.12.7309 · Cited by 1,564
Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis
Dragovic, R., Gardiner, C., Brooks, A. S., Tannetta, D., Ferguson, D. J. P., Hole, P., Carr, B., Redman, C. W. G., Harris, A. L., Dobson, P. J., et al. (2011) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2011.04.003 · Cited by 1,368
Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation
Kalra, H., Simpson, R. J., Ji, H., Aikawa, E., Altevogt, P., Askenase, P., Bond, V. C., Borràs, F. E., Breakefield, X., Budnik, V., et al. (2012) · PLoS Biology · doi:10.1371/journal.pbio.1001450 · Cited by 1,367
Miyazaki, J., Araki, K., Yamato, E., Ikegami, H., Asano, T., Shibasaki, Y., Oka, Y., & Yamamura, K. (1990) · Endocrinology · doi:10.1210/endo-127-1-126 · Cited by 1,267
Gibbings, D., Ciaudo, C., Erhardt, M., & Voinnet, O. (2009) · Nature Cell Biology · doi:10.1038/ncb1929 · Cited by 1,037
Record, M., Carayon, K., Poirot, M., & Silvente‐Poirot, S. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2013.10.004 · Cited by 893
Takahashi, Y., Nishikawa, M., Shinotsuka, H., Matsui, Y., Ohara, S., Imai, T., & Takakura, Y. (2013) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2013.03.013 · Cited by 743
Endogenous RNAs modulate microRNA sorting to exosomes and transfer to acceptor cells.
Squadrito, M. L., Baer, C., Burdet, F., Maderna, C., Gilfillan, G. D., Lyle, R., Ibberson, M., & De Palma, M. (2014) · Cell Reports · doi:10.1016/j.celrep.2014.07.035 · Cited by 604
Adipose Tissue Exosome-Like Vesicles Mediate Activation of Macrophage-Induced Insulin Resistance
Deng, Z., Poliakov, A., Hardy, R. W., Clements, R. H., Liu, C., Liu, Y., Wang, J., Xiang, X., Zhang, S., Zhuang, X., et al. (2009) · Diabetes · doi:10.2337/db09-0216 · Cited by 596
Imai, T., Takahashi, Y., Nishikawa, M., Kato, K., Morishita, M., Yamashita, T., Matsumoto, A., Charoenviriyakul, C., & Takakura, Y. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.26238 · Cited by 592
Bobrie, A., Colombo, M., Krumeich, S., Raposo, G., & Théry, C. (2012) · Journal of Extracellular Vesicles · doi:10.3402/jev.v1i0.18397 · Cited by 581
Nontemplated Nucleotide Additions Distinguish the Small RNA Composition in Cells from Exosomes
Koppers-Lalic, D., Hackenberg, M., Bijnsdorp, I. V., van Eijndhoven, M. A. J., Sadek, P., Sie, D., Zini, N., Middeldorp, J. M., Ylstra, B., de Menezes, R. X., et al. (2014) · Cell Reports · doi:10.1016/j.celrep.2014.08.027 · Cited by 560
Regulation of pancreas development by hedgehog signaling
Hebrok, M., Kim, S. K., St-Jacques, B., McMahon, A. P., & Melton, D. A. (2000) · Development · doi:10.1242/dev.127.22.4905 · Cited by 412
Handschin, C., Choi, C. S., Chin, S., Kim, S., Kawamori, D., Kurpad, A. J., Neubauer, N., Hu, J., Mootha, V. K., Kim, Y.-B., et al. (2007) · Journal of Clinical Investigation · doi:10.1172/jci31785 · Cited by 335
C2C12 myoblasts release micro-vesicles containing mtDNA and proteins involved in signal transduction
Guescini, M., Guidolin, D., Vallorani, L., Casadei, L., Gioacchini, A. M., Tibollo, P., Battistelli, M., Falcieri, E., Battistin, L., Agnati, L. F., et al. (2010) · Experimental Cell Research · doi:10.1016/j.yexcr.2010.04.006 · Cited by 294
Hedgehog signalling: Emerging evidence for non-canonical pathways
Jenkins, D. (2009) · Cellular Signalling · doi:10.1016/j.cellsig.2009.01.033 · Cited by 234
Forterre, A. V., Jalabert, A., Chikh, K., Pesenti, S., Euthine, V., Granjon, A., Errazuriz, E., Lefai, É., Vidal, H., & Rome, S. (2013) · Cell Cycle · doi:10.4161/cc.26808 · Cited by 191
Self-citationAswad, H., Forterre, A., Wiklander, O. P. B., Vial, G., Danty-Berger, E., Jalabert, A., Lamazière, A., Meugnier, E., Pesenti, S., Ott, C., et al. (2014) · Diabetologia · doi:10.1007/s00125-014-3337-2 · Cited by 185
Self-citationForterre, A. V., Jalabert, A., Berger, E., Baudet, M., Chikh, K., Errazuriz-Cerda, E., Larichaudy, J. D., Chanon, S., Weiss‐Gayet, M., Hesse, A.-M., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0084153 · Cited by 176
Self-citationVega-López, S., Ausman, L. M., Jalbert, S. M., Erkkilä, A. T., & Lichtenstein, A. H. (2006) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/84.1.54 · Cited by 160
Horizontal transfer of exosomal microRNAs transduce apoptotic signals between pancreatic beta-cells
Guay, C., Menoud, V., Rome, S., & Regazzi, R. (2015) · Cell Communication and Signaling · doi:10.1186/s12964-015-0097-7 · Cited by 158
Self-citationLe Bihan, M.-C., Bigot, A., Jensen, S. S., Dennis, J. L., Rogowska-Wrzesinska, A., Lainé, J., Gache, V., Furling, D., Jensen, O. N., Voit, T., et al. (2012) · Journal of Proteomics · doi:10.1016/j.jprot.2012.09.008 · Cited by 153
Hedgehog signaling regulation of insulin production by pancreatic beta-cells.
Thomas, M. K., Rastalsky, N., Lee, J. H., & Habener, J. F. (2000) · Diabetes · doi:10.2337/diabetes.49.12.2039 · Cited by 153
Dif, N., Euthine, V., Gonnet, E., Laville, M., Vidal, H., & Lefai, É. (2006) · Biochemical Journal · doi:10.1042/bj20060499 · Cited by 148
Self-citationGranjon, A., Gustin, M., Rieusset, J., Lefai, É., Meugnier, E., Güller, I., Cerutti, C., Paultre, C. Z., Disse, E., RABASA-LHORET, R. P. R., et al. (2009) · Diabetes · doi:10.2337/db09-0165 · Cited by 141
Self-citationDifferential Gene Expression in Well-Regulated and Dysregulated Pancreatic β-Cell (MIN6) Sublines
Lilla, V., Webb, G. C., Rickenbach, K., Maturana, A. D., Steiner, D. F., Halban, P. A., & Irminger, J.-C. (2003) · Endocrinology · doi:10.1210/en.2002-220916 · Cited by 129
Bouzakri, K., Plomgaard, P., Berney, T., Donath, M. Y., Pedersen, B. K., & Halban, P. A. (2011) · Diabetes · doi:10.2337/db10-1178 · Cited by 122
Trazzi, S., Mitrugno, V. M., Valli, E., Fuchs, C., Rizzi, S., Guidi, S., Perini, G., Bartesaghi, R., & Ciani, E. (2011) · Human Molecular Genetics · doi:10.1093/hmg/ddr033 · Cited by 114
Exosomes: mediators of communication in eukaryotes
Lopez‐Verrilli, M. A., & Court, F. A. (2013) · Biological Research · doi:10.4067/s0716-97602013000100001 · Cited by 101
Defect of insulin signal in peripheral tissues: Important role of ceramide
Hassan, R. H. (2014) · World Journal of Diabetes · doi:10.4239/wjd.v5.i3.244 · Cited by 97
Hedgehog signaling in pancreas development and disease
Lau, J., Kawahira, H., & Hebrok, M. (2006) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-005-5357-z · Cited by 95
Rudin, C. M. (2012) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-12-0568 · Cited by 93
HOW TO CHOOSE A NORMALIZATION STRATEGY FOR MIRNA QUANTITATIVE REAL-TIME (QPCR) ARRAYS
Deo, A., Carlsson, J., & Lindlöf, A. (2011) · Journal of Bioinformatics and Computational Biology · doi:10.1142/s0219720011005793 · Cited by 56
Müller, G. A., Schneider, M., Biemer‐Daub, G., & Wied, S. (2011) · Obesity · doi:10.1038/oby.2011.29 · Cited by 45
Forterre, A. V., Jalabert, A., Berger, E., Baudet, M., Chikh, K., Errazuriz-Cerda, E., Larichaudy, J. D., Chanon, S., Weiss‐Gayet, M., Hesse, A.-M., et al. (2014) · PLoS ONE · doi:10.1371/annotation/ecd1e074-2618-4ad0-95c0-efdb467c714b · Cited by 43
Self-citationKubota, S., Chiba, M., Watanabe, M., Sakamoto, M., & Watanabe, N. (2014) · Oncology Reports · doi:10.3892/or.2014.3605 · Cited by 30
Mai, G., Bucher, P., Morel, P., Mei, J., Bosco, D., Andrès, A., Máthé, Z., Wekerle, T., Berney, T., & Bühler, L. H. (2005) · American Journal of Transplantation · doi:10.1111/j.1600-6143.2005.00795.x · Cited by 23
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Kang, M., Jordan, V., Blenkiron, C., & Chamley, L. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12085 · Cited by 519
Roles and mechanisms of exosomal non-coding RNAs in human health and diseases
Li, C., Ni, Y., Xu, H., Xiang, Q., Zhao, Y. Q., Zhan, J., He, J., Li, S., & Liu, Y. (2021) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-021-00779-x · Cited by 424
Extracellular Vesicles: Novel Mediators of Cell Communication In Metabolic Disease
Huang‐Doran, I., Zhang, C., & Vidal‐Puig, A. (2016) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2016.10.003 · Cited by 387
Extracellular Vesicles: Exosomes and Microvesicles, Integrators of Homeostasis
Stahl, P. D., & Raposo, G. (2019) · Physiology · doi:10.1152/physiol.00045.2018 · Cited by 383
High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Kumar, A., Sundaram, K., Mu, J., Dryden, G. W., Sriwastva, M. K., Lei, C., Zhang, L., Qiu, X., Xu, F., Yan, J., et al. (2021) · Nature Communications · doi:10.1038/s41467-020-20500-w · Cited by 276
Metadata
- Authors
- Jalabert, A., Vial, G., Guay, C., Wiklander, O. P. B., Nordin, J. Z., Aswad, H., Forterre, A., Meugnier, E., Pesenti, S., Regazzi, R., Danty-Berger, E., Ducreux, S., Vidal, H., El-Andaloussi, S., Rieusset, J., & Rome, S.
- Affiliations
- Inserm (FR)
- University of Lausanne (CH)
- Karolinska University Hospital (SE)
- Corresponding author
- Rome, Sophie
- ORCID
- Venue
- Diabetologia
- Published
- 2016-02-06
- Volume
- 59
- Issue
- 5
- Pages
- 1049-1058
- ISSN
- 0012-186X (ISSN-L)
- 1432-0428
- OpenAlex
- W2260637370
- Cited by
- 177
- References
- 47
- Citations by year
- 2026: 9 · 2025: 20 · 2024: 18 · 2023: 19 · 2022: 20 · 2021: 24 · 2020: 17 · 2019: 19 · 2018: 15 · 2017: 11 · 2016: 5
- FWCI
- 8.67
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Diabetologia (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- HAL (submittedVersion)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Funders
- AstraZeneca
- Fondation pour la Recherche Médicale (DRM20101220456)
- Université de Lyon
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Keywords
- exosome-like nanoparticles, skeletal muscle, pancreatic beta cells, insulin resistance, microRNA transfer, miR-16, PTCH1, islet development, beta-cell proliferation, diabetes mellitus
- MeSH terms
- Animals, Cell Line, Insulin Resistance, Male, Muscle, Skeletal, Muscle Fibers, Skeletal, MicroRNAs, Insulin-Secreting Cells, Mice, Exosomes
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