The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the international society for extracellular vesicles.
Abstract
Secreted membrane‐enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non‐vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Particles isolated from human and mouse serum by ExoQuick complied with the characteristics of small extracellular vesicles.
“In short, particles obtained from human and C57BL/6 mouse serum by ExoQuick complied with the characteristics of sEVs (Lötvall et al., 2014).”
From Acute Cerebral Ischemia Increases a Set of Brain-Specific miRNAs in Serum Small Extracellular Vesicles (2022) · cites this paper as Lötvall et al., 2014
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Self-citationVesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation
Kalra, H., Simpson, R. J., Ji, H., Aikawa, E., Altevogt, P., Askenase, P., Bond, V. C., Borràs, F. E., Breakefield, X., Budnik, V., et al. (2012) · PLoS Biology · doi:10.1371/journal.pbio.1001450 · Cited by 1,367
Self-citation
Flagged first, then by citations
Research works printed but not linked: 19
- 10.1038/nrm3335 · DOI
- 10.1161/ATVBAHA.112.300139 · DOI
- Grein SG, 2014, “Small Talk” in the innate immune system via RNA‐containing extracellular vesicles, Front Immunol, 5, 542
- 10.1073/pnas.1019055108 · DOI · Paper page
- 10.1038/ncb2210 · DOI · Paper page
- 10.1002/pmic.201300282 · DOI
- 10.3402/jev.v3.24858 · DOI
- Zonneveld MI, 2014, Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures, J Extracell Vesicles, 3, 10.3402/jev.v3.24215 · DOI
- 10.3402/jev.v2i0.20389 · DOI
- 10.3402/jev.v2i0.20360 · DOI
- Hill AF, 2014, ISEV position paper: extracellular vesicle RNA analysis and bioinformatics, J Extracell Vesicles, 2
- Kim DK, 2014, EVpedia: a community web portal for extracellular vesicles research, Bioinformatics
- 10.1016/j.tins.2006.02.005 · DOI
- 10.3402/jev.v3.23111 · DOI
- 10.1016/j.yexcr.2009.03.010 · DOI
- 10.3402/jev.v3.24783 · DOI
- 10.1016/j.ajpath.2012.07.030 · DOI
- 10.1074/jbc.272.27.17216 · DOI
- 10.1158/0008-5472.CAN-12-0925 · DOI
Cited by
Sorted by citations
Théry, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin‐Smith, G. K., et al. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1535750 · Cited by 11,099
Self-citationCommunication by Extracellular Vesicles: Where We Are and Where We Need to Go
Tkach, M., & Théry, C. (2016) · Cell · doi:10.1016/j.cell.2016.01.043 · Cited by 3,444
Self-citationGlypican-1 identifies cancer exosomes and detects early pancreatic cancer
Melo, S. A., Luecke, L. B., Kahlert, C., Fernandez, A. F., Gammon, S. T., Kaye, J., LeBleu, V. S., Mittendorf, E. A., Weitz, J., Rahbari, N., et al. (2015) · Nature · doi:10.1038/nature14581 · Cited by 2,880
Concise review: MSC-derived exosomes for cell-free therapy
Phinney, D. G., & Pittenger, M. F. (2017) · Stem Cells · doi:10.1002/stem.2575 · Cited by 1,834
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research
EV-TRACK Consortium, Van Deun, J., Mestdagh, P., Agostinis, P., Akay, Ö., Anand, S., Anckaert, J., Martinez, Z. A., Baetens, T., Beghein, E., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4185 · Cited by 1,284
Self-citationTherapeutically harnessing extracellular vesicles
Cheng, L., & Hill, A. F. (2022) · Nature Reviews Drug Discovery · doi:10.1038/s41573-022-00410-w · Cited by 737
Self-citationExtracellular vesicles round off communication in the nervous system
Budnik, V., Ruiz-Cañada, C., & Wendler, F. (2016) · Nature Reviews Neuroscience · doi:10.1038/nrn.2015.29 · Cited by 712
Kim, M. S., Haney, M. J., Zhao, Y., Yuan, D., Deygen, I., Klyachko, N. L., Kabanov, A. V., & Batrakova, E. V. (2018) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2017.09.011 · Cited by 663
Tian, Y., Gong, M., Hu, Y., Liu, H., Zhang, W., Zhang, M., Hu, X., Aubert, D., Zhu, S., Wu, L., et al. (2020) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2019.1697028 · Cited by 643
Mesenchymal stromal cell secretome: influencing therapeutic potential by cellular pre-conditioning
Ferreira, J. R., Teixeira, G. Q., Santos, S. G., Barbosa, M. A., Almeida-Porada, G., & Gonçalves, R. M. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02837 · Cited by 572
Targeting and Crossing the Blood-Brain Barrier with Extracellular Vesicles
Saint-Pol, J., Gosselet, F., Duban-Deweer, S., Pottiez, G., & Karamanos, Y. (2020) · Cells · doi:10.3390/cells9040851 · Cited by 503
Exosome-mediated delivery of hydrophobically modified siRNA for huntingtin mRNA silencing
Didiot, M.-C., Hall, L. M., Coles, A. H., Haraszti, R. A., Godinho, B. M., Chase, K., Sapp, E., Ly, S., Alterman, J. F., Hassler, M. R., et al. (2016) · Molecular Therapy · doi:10.1038/mt.2016.126 · Cited by 483
Evidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine
Fais, S., O’Driscoll, L., Borras, F. E., Buzas, E., Camussi, G., Cappello, F., Carvalho, J., Cordeiro da Silva, A., Del Portillo, H., El Andaloussi, S., et al. (2016) · ACS Nano · doi:10.1021/acsnano.5b08015 · Cited by 481
The potential of endurance exercise-derived exosomes to treat metabolic diseases
Safdar, A., Saleem, A., & Tarnopolsky, M. A. (2016) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2016.76 · Cited by 463
Extracellular vesicles in neurodegenerative disease — pathogenesis to biomarkers
Thompson, A. G., Gray, E., Heman-Ackah, S. M., Mäger, I., Talbot, K., Andaloussi, S. E., Wood, M. J., & Turner, M. R. (2016) · Nature Reviews Neurology · doi:10.1038/nrneurol.2016.68 · Cited by 431
Physical exercise induces rapid release of small extracellular vesicles into the circulation
Frühbeis, C., Helmig, S., Tug, S., Simon, P., & Krämer‐Albers, E. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.28239 · Cited by 360
Donor dendritic cell–derived exosomes promote allograft-targeting immune response
Liu, Q., Rojas-Canales, D. M., Divito, S. J., Shufesky, W. J., Stolz, D. B., Erdos, G., Sullivan, M. L. G., Gibson, G. A., Watkins, S. C., Larregina, A. T., et al. (2016) · Journal of Clinical Investigation · doi:10.1172/jci84577 · Cited by 302
The function and clinical application of extracellular vesicles in innate immune regulation
Zhou, X., Xie, F., Wang, L., Zhang, L., Zhang, S., Fang, M., & Zhou, F. (2020) · Cellular & Molecular Immunology · doi:10.1038/s41423-020-0391-1 · Cited by 294
Upadhya, R., Zingg, W., Shetty, S., & Shetty, A. K. (2020) · Journal of Controlled Release · doi:10.1016/j.jconrel.2020.04.017 · Cited by 282
Candida albicans biofilm–induced vesicles confer drug resistance through matrix biogenesis
Zarnowski, R., Sanchez, H., Covelli, A. S., Dominguez, E., Jaromin, A., Bernhardt, J., Mitchell, K. F., Heiss, C., Azadi, P., Mitchell, A., et al. (2018) · PLOS Biology · doi:10.1371/journal.pbio.2006872 · Cited by 260
Cellular Mechanisms of Liver Fibrosis
Acharya, P., Chouhan, K., Weiskirchen, S., & Weiskirchen, R. (2021) · Frontiers in Pharmacology · doi:10.3389/fphar.2021.671640 · Cited by 252
Combining Machine Learning and Nanofluidic Technology To Diagnose Pancreatic Cancer Using Exosomes
Ko, J., Bhagwat, N., Yee, S. S., Ortiz, N., Sahmoud, A., Black, T., Aiello, N. M., McKenzie, L., O’Hara, M., Redlinger, C., et al. (2017) · ACS Nano · doi:10.1021/acsnano.7b05503 · Cited by 249
Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90
Zhang, G., Liu, Z., Ding, H., Zhou, Y., Doan, H. A., Sin, K. W. T., Zhu, Z. J., Flores, R., Wen, Y., Gong, X., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-00726-x · Cited by 248
Improved Loading of Plasma-Derived Extracellular Vesicles to Encapsulate Antitumor miRNAs
Pomatto, M. A. C., Bussolati, B., D’Antico, S., Ghiotto, S., Tetta, C., Brizzi, M. F., & Camussi, G. (2019) · Molecular Therapy - Methods & Clinical Development · doi:10.1016/j.omtm.2019.01.001 · Cited by 243
Wang, Y., Cao, Z., Wei, Q., Ma, K., Hu, W., Huang, Q., Su, J., Li, H., Zhang, C., & Fu, X. (2022) · Acta Biomaterialia · doi:10.1016/j.actbio.2022.05.018 · Cited by 225
Exosomes as Mediators of the Systemic Adaptations to Endurance Exercise
Safdar, A., & Tarnopolsky, M. A. (2018) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a029827 · Cited by 203
Ngolab, J., Trinh, I., Rockenstein, E., Mante, M., Florio, J., Trejo, M., Masliah, D., Adame, A., Masliah, E., & Rissman, R. A. (2017) · Acta Neuropathologica Communications · doi:10.1186/s40478-017-0445-5 · Cited by 200
RetractedWang, J.-H., Forterre, A. V., Zhao, J., Frimannsson, D. O., Delcayre, A., Antes, T. J., Efron, B., Jeffrey, S. S., Pegram, M. D., & Matin, A. C. (2018) · Molecular Cancer Therapeutics · doi:10.1158/1535-7163.mct-17-0827 · Cited by 181
Jalabert, A., Vial, G., Guay, C., Wiklander, O. P. B., Nordin, J. Z., Aswad, H., Forterre, A., Meugnier, E., Pesenti, S., Regazzi, R., et al. (2016) · Diabetologia · doi:10.1007/s00125-016-3882-y · Cited by 177
Bhome, R., Goh, R. W., Bullock, M. D., Pillar, N., Thirdborough, S. M., Mellone, M., Mirnezami, R., Galea, D., Veselkov, K., Gu, Q., et al. (2017) · Aging · doi:10.18632/aging.101355 · Cited by 155
Sutaria, D. S., Badawi, M., Phelps, M. A., & Schmittgen, T. D. (2017) · Pharmaceutical Research · doi:10.1007/s11095-017-2123-5 · Cited by 140
Mesenchymal Stem Cell-Derived Extracellular Vesicles to the Rescue of Renal Injury
Birtwistle, L., Chen, X.-M., & Pollock, C. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22126596 · Cited by 113
McNamara, R. P., Costantini, L. M., Myers, T. A., Schouest, B., Maness, N. J., Griffith, J. D., Damania, B. A., MacLean, A. G., & Dittmer, D. P. (2018) · mBio · doi:10.1128/mbio.02344-17 · Cited by 109
Astrocyte-derived extracellular vesicles: A double-edged sword in central nervous system disorders
Zhao, S., Sheng, S., Wang, Y., Ding, L., Xu, X., Xia, X., & Zheng, J. C. (2021) · Neuroscience & Biobehavioral Reviews · doi:10.1016/j.neubiorev.2021.02.027 · Cited by 90
Marcoux, G., Magron, A., Sut, C., Laroche, A., Laradi, S., Hamzeh‐Cognasse, H., Allaeys, I., Cabon, O., Julien, A., Garraud, O., et al. (2019) · Transfusion · doi:10.1111/trf.15300 · Cited by 87
Lee, E. J., Choi, Y., Lee, H. J., Hwang, D. W., & Lee, D. S. (2022) · Journal of Nanobiotechnology · doi:10.1186/s12951-022-01356-2 · Cited by 89
Extracellular Vesicle Proteins and MicroRNAs as Biomarkers for Traumatic Brain Injury
Guedes, V. A., Devoto, C., Leete, J., Sass, D., Acott, J. D., Mithani, S., & Gill, J. M. (2020) · Frontiers in Neurology · doi:10.3389/fneur.2020.00663 · Cited by 85
Extracellular vesicles in renal tissue damage and regeneration
Bruno, S., Porta, S., & Bussolati, B. (2016) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2016.06.058 · Cited by 74
MicroRNAs and miRceptors: a new mechanism of action for intercellular communication
Fabbri, M. (2018) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2016.0486 · Cited by 65
A common vesicle proteome drives fungal biofilm development
Zarnowski, R., Sanchez, H., Jaromin, A., Zarnowska, U. J., Nett, J. E., Mitchell, A. P., & Andes, D. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2211424119 · Cited by 63
Selection of urinary sediment miRNAs as specific biomarkers of IgA nephropathy
Duan, Z.-Y., Cai, G.-Y., Bu, R., Lu, Y., Hou, K., & Chen, X.-M. (2016) · Scientific Reports · doi:10.1038/srep23498 · Cited by 61
Filannino, F. M., Panaro, M. A., Benameur, T., Pizzolorusso, I., & Porro, C. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25031629 · Cited by 60
Desjardins, P., Berthiaume, R., Couture, C., Le-Bel, G., Roy, V., Gros-Louis, F., Moulin, V. J., Proulx, S., Chemtob, S., Germain, L., et al. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232012201 · Cited by 39
“In our present study, all three exosome markers were found to be expressed by hCECs and hCFs exosomes but not by hCEnCs that were positive for CD63 and CD9 but not for CD81.”
BackgroundInfluentialPlatelet-derived extracellular vesicles in Huntington’s disease
Denis, H. L., Lamontagne-Proulx, J., St-Amour, I., Mason, S. L., Weiss, A., Chouinard, S., Barker, R. A., Boilard, E., & Cicchetti, F. (2018) · Journal of Neurology · doi:10.1007/s00415-018-9022-5 · Cited by 32
Yu, X., Elfimova, N., Müller, M., Bachurski, D., Koitzsch, U., Drebber, U., Mahabir, E., Hansen, H. P., Friedman, S. L., Klein, S., et al. (2022) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2022.02.013 · Cited by 27
Recent Progress in Extracellular Vesicle-Based Carriers for Targeted Drug Delivery in Cancer Therapy
Tang, Y., Liu, X., Sun, M., Xiong, S., Xiao, N., Li, J., He, X., & Xie, J. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15071902 · Cited by 24
Recent advances in our understanding of the allograft response
Hennessy, C., Lewik, G., Cross, A., Hester, J., & Issa, F. (2021) · Faculty Reviews · doi:10.12703/r/10-21 · Cited by 18
Zhou, X., Xu, C., Chao, D., Chen, Z., Li, S., Shi, M., Pei, Y., Dai, Y., Ji, J., Ji, Y., et al. (2022) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2022.874903 · Cited by 13
Lucas, F., Gil‐Pulido, J., LaMacchia, J., Preffer, F., Wallace, P. K., & Lopez, P. (2020) · Cytometry Part A · doi:10.1002/cyto.a.23940 · Cited by 10
Isolation of Extracellular Vesicles Derived from Mesenchymal Stromal Cells by Ultracentrifugation
Ramirez-Bajo, M., Banon-Maneus, E., Rovira, J., Campistol, J. M., & Diekmann, F. (2020) · BIO-PROTOCOL · doi:10.21769/bioprotoc.3860 · Cited by 10
Metadata
- Authors
- Lötvall, J., Hill, A. F., Hochberg, F., Buzás, E. I., Di Vizio, D., Gardiner, C., Gho, Y. S., Kurochkin, I. V., Mathivanan, S., Quesenberry, P., Sahoo, S., Tahara, H., Wauben, M. H., Witwer, K. W., & Théry, C.
- Affiliations
- Krefting Research Centre, University of Gothenburg Göteborg Sweden
- Department of Biochemistry and Molecular Biology Bio21 Molecular Science and Biotechnology Institute, University of Melbourne Parkville Australia
- Department of Neurosurgery University of California at San Diego San Diego CA USA
- Department of Genetics Cell‐ and Immunobiology, Semmelweis University Budapest Hungary
- Cedars‐Sinai Medical Center Los Angeles CA USA
- Nuffield Department of Obstetrics and Gynaecology University of Oxford Oxford UK
- Department of Life Sciences Pohang University of Science and Technology Pohang Republic of Korea
- Bioinformatics Institute, Singapore Singapore
- Department of Biochemistry La Trobe University Melbourne Australia
- The Warren Alpert Medical School of Brown University Providence RI USA
- Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai New York NY USA
- Department of Cellular and Molecular Biology Hiroshima University Institute of Biomedical & Health Sciences Hiroshima Japan
- Department of Biochemistry and Cell Biology, Faculty of Veterinary medicine Utrecht University Utrecht The Netherlands
- Department of Molecular and Comparative Pathobiology Johns Hopkins School of Medicine Baltimore MD USA
- INSERM U932, Institut Curie 26 rue d'Ulm 75005 Paris France
- Venue
- Journal of Extracellular Vesicles
- Publisher
- Wiley
- Published
- 2014-01
- Volume
- 3
- Issue
- 1
- ISSN
- 2001-3078
- PMC
- PMC4275645
- Cited by
- 2,451
- References
- 2
- Language
- en
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
