Functional analysis of the calcineurin-encoding gene cnaA from Aspergillus oryzae: evidence for its putative role in stress adaptation
Abstract
The presence of putative STRE (stress response regulatory element) and HSF (heat-shock factor) transcription factor binding sites in the promoter region of the gene encoding calcineurin ( cnaA) from Aspergillus oryzae implicated a probable role for calcineurin in the stress response. The activity of calcineurin was enhanced during growth of the wild-type strain in the presence of 1 M NaCl (2.6-fold), at alkaline pH 10.0 (2.9-fold) and at 37 degrees C (1.6-fold). The induction of cnaA antisense expression resulted in reduced calcineurin activity (1.4-fold) and caused a growth defect under the stress conditions. Induction of a strain overexpressing cnaA resulted in an increase in calcineurin activity under stress conditions, such as the presence of 1 M NaCl (73%), alkaline pH 10.0 (70%), and a temperature of 37 degrees C (50%), in addition to tolerance to FK506 (a specific inhibitor of calcineurin). While a role for calcineurin in hyphal growth is well recognized, the present study suggests that stress adaptation mechanisms in filamentous fungi involve calmodulin/calcineurin-mediated signal transduction pathways.
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Calcineurin is implicated in regulation of growth under alkaline pH, high salt concentrations and heat stress in filamentous fungi.
“Subsequently calcineurin was also implicated in the regulation growth under alkaline pH, high salt concentrations and heat stress conditions in filamentous fungi (Juvvadi et al., 2003).”
From Calcineurin as a multifunctional regulator: Unraveling novel functions in fungal stress responses, hyphal growth, drug resistance, and pathogenesis (2014) · cites this paper as Juvvadi et al., 2003
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- Authors
- Juvvadi, P. R., Kuroki, Y., Arioka, M., Nakajima, H., & Kitamoto, K.
- Affiliations
- The University of Tokyo (JP)
- Corresponding author
- Kitamoto, Katsuhiko
- Venue
- Archives of Microbiology
- Published
- 2003-04-23
- Volume
- 179
- Issue
- 6
- Pages
- 416-422
- ISSN
- 0003-9276 (ISSN-L)
- 0302-8933
- OpenAlex
- W1885923855
- Cited by
- 44
- References
- 42
- Citations by year
- 2025: 1 · 2023: 2 · 2022: 2 · 2021: 1 · 2018: 1 · 2017: 2 · 2016: 5 · 2015: 2 · 2014: 4 · 2013: 3 · 2012: 2
- FWCI
- 0.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Archives of Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- calcineurin, Aspergillus oryzae, stress adaptation, promoter analysis, NaCl tolerance, alkaline tolerance, heat tolerance, antisense expression, FK506, hyphal growth, filamentous fungi
- MeSH terms
- Adaptation, Physiological, Aspergillus oryzae, Base Sequence, DNA, Fungal, Genes, Fungal, Hydrogen-Ion Concentration, Molecular Sequence Data, Sodium Chloride, Temperature, Gene Expression Regulation, Fungal, Sequence Alignment, Calcineurin
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