The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
How reliable are G-protein-coupled receptor antibodies?
Open accessAbstract
A cluster of manuscripts in this issue of the Journal highlights a lack of selectivity of 49 antibodies against 19 subtypes of alpha(1)- and beta-adrenoceptors, muscarinic, dopamine and galanin receptors as well as vanilloid (TRPV1) receptors. Taken together these data demonstrate that lack of selectivity appears to be the rule rather than the exception for antibodies against G-protein-coupled and perhaps also other receptors. Thus, the previously often applied validation of such antibodies by the disappearance of staining in the presence of blocking peptide, i.e. the antigen against which the antibody was raised, alone is insufficient to demonstrate specificity. We propose that receptor antibodies should be validated by at least one of the following techniques: a) disappearance of staining in knock-out animals of the target receptor, b) reduction of staining upon knock-down approaches such as siRNA treatment, c) selectivity of staining in immunoblots or immunocytochemistry for the target receptor vs. related subtypes when expressed in the same cell line and/or d) antibodies raised against multiple distinct epitopes of a receptor yielding very similar staining patterns. Other issues of consideration to obtain reliable results based on receptor antibodies in applications such as immunohistochemistry or immunoblotting are also being discussed.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“However, there is uncertainty around the usefulness of antibodies against GPCRs (Michel et al., 2009”
From Self-reported symptom burden in postural orthostatic tachycardia syndrome (POTS): A narrative review of observational and interventional studies (2023) · cites this paper as Michel et al., 2009
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Chen, W., Mahadomrongkul, V., Berger, U. V., Bassan, M., DeSilva, T. M., Tanaka, K., Irwin, N., Aoki, C. J., & Rosenberg, P. A. (2004) · Journal of Neuroscience · doi:10.1523/jneurosci.1586-03.2004 · Cited by 269
Impact of GPCRs in clinical medicine: Monogenic diseases, genetic variants and drug targets
Insel, P. A., Tang, C., Hahntow, I. N., & Michel, M. C. (2006) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2006.09.029 · Cited by 178
Self-citationTen commercial antibodies for alpha-1-adrenergic receptor subtypes are nonspecific
Jensen, B. C., Swigart, P. M., & Simpson, P. C. (2009) · Naunyn-Schmiedeberg's Archives of Pharmacology · doi:10.1007/s00210-008-0368-6 · Cited by 176
Antibodies as valuable neuroscience research tools versus reagents of mass distraction
Rhodes, K. J., & Trimmer, J. S. (2006) · The Journal of Neuroscience · doi:10.1523/jneurosci.2728-06.2006 · Cited by 162
Expression and functional role of β-adrenoceptors in the human urinary bladder urothelium
Otsuka, A., Shinbo, H., Matsumoto, R., KURITA, Y., & Ozono, S. (2008) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/s00210-008-0274-y · Cited by 162
Pradidarcheep, W., Stallen, J., Labruyère, W. T., Dabhoiwala, N. F., Michel, M. C., & Lamers, W. H. (2009) · Naunyn-Schmiedeberg's Archives of Pharmacology · doi:10.1007/s00210-009-0393-0 · Cited by 152
Self-citationDistribution and regulation of protein expression of the free fatty acid receptor GPR120
Miyauchi, S., Hirasawa, A., Iga, T., Liu, N., Itsubo, C., Sadakane, K., Hara, T., & Tsujimoto, G. (2009) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/s00210-008-0390-8 · Cited by 146
Self-citation1-Adrenergic receptors prevent a maladaptive cardiac response to pressure overload
O’Connell, T. D. (2006) · Journal of Clinical Investigation · doi:10.1172/jci22811 · Cited by 143
Jositsch, G., Papadakis, T., Haberberger, R. V., Wolff, M., Wess, J., & Kummer, W. (2008) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/s00210-008-0365-9 · Cited by 143
Tanaka, T., Yano, T., Adachi, T., Koshimizu, T., Hirasawa, A., & Tsujimoto, G. (2008) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/s00210-007-0250-y · Cited by 120
Self-citation
Cited by
Sorted by citations
Items 301–305 of 305
Importance de la S-nitrosation des récepteurs cérébrovasculaires de l’angiotensine II
Bouressam, M. (2018) · theses.fr (ABES) · doi:10.70675/ea40fd3bz38a3z4244zaca8z260f194edc85
Expression of GPR34 in microglia remains stable in human Alzheimer’s disease
Seiffer, S., Rotter, J., Brendler, J., Ricken, A. M., Detzer, Z., Braune, M., Schöneberg, T., Schulz, A., Winter, K., & Bechmann, I. (2026) · Acta Neuropathologica · doi:10.1007/s00401-026-03035-0
Increased Adrenergic Receptor Expression in Meniere Disease
Xiao, A. Y., Kanaris, A. A., Ishii, M., Leković, G. P., López, I. A., Ishiyama, G. P., & Ishiyama, A. (2026) · Otology & Neurotology · doi:10.1097/mao.0000000000005010
Metadata
- Authors
- Michel, M. C., Wieland, T., & Tsujimoto, G.
- Affiliations
- Amsterdam UMC Location University of Amsterdam (NL)
- Heidelberg University (DE)
- Kyoto University (JP)
- Corresponding author
- Michel, Martin C.
- Venue
- Naunyn-Schmiedeberg's Archives of Pharmacology
- Published
- 2009-01-27
- Volume
- 379
- Issue
- 4
- Pages
- 385-388
- ISSN
- 0028-1298 (ISSN-L)
- 1432-1912
- OpenAlex
- W2029187954
- Cited by
- 306
- References
- 28
- Citations by year
- 2026: 2 · 2025: 11 · 2024: 18 · 2023: 19 · 2022: 19 · 2021: 23 · 2020: 16 · 2019: 19 · 2018: 14 · 2017: 21 · 2016: 14 · 2015: 30 · 2014: 23 · 2013: 18 · 2012: 21
- FWCI
- 7.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Naunyn-Schmiedeberg s Archives of Pharmacology (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Pure Amsterdam UMC (publishedVersion)
- UvA-DARE (University of Amsterdam) (acceptedVersion, other-oa)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- G protein-coupled receptors, antibody specificity, cross-reactivity, adrenergic receptors, muscarinic receptors, dopamine receptors, galanin receptors, TRPV1, blocking peptides, siRNA knockdown, epitope specificity, immunohistochemistry, Western blot
- MeSH terms
- Animals, Antibodies, Antibody Specificity, Humans, Receptors, Adrenergic, Receptors, Dopamine, Receptors, Muscarinic, Conserved Sequence, Sequence Homology, Amino Acid, Editorial Policies, Receptors, G-Protein-Coupled, Receptors, Galanin, TRPV Cation Channels, Validation Studies as Topic
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
