Lipid nanoparticles-encapsulated brain-derived neurotrophic factor mRNA delivered through the round window niche in the cochleae of guinea pigs
Open accessAbstract
The treatment of sensorineural hearing loss (SNHL) may be achieved via the application of a cochlear implant (CI) that allows the electrical stimulation of spiral ganglion neurons (SGNs). Nevertheless, the efficacy of CIs is limited by the degeneration of SGNs following SNHL. Although the application of exogenous neurotrophic factors has been reported to decrease SGN degeneration, non-invasive targeted drug delivery systems are required to achieve effective results. In this study, an SS-cleavable proton-activated lipid-like material [ssPalm; a neutral lipid nanoparticle (LNP)], was loaded with mRNA, and the efficacy of this material as a delivery system was investigated. Our results showed that LNP ssPalm carrying brain-derived neurotrophic factor (BDNF) mRNA was suitable for the treatment of inner ear diseases, preventing the degeneration of SGNs. In conclusion, this modern nanotechnology-based bioconjugation system, LNP ssPalm, is a potential non-invasive targeted therapy allowing the delivering biomaterials to specific structures within the inner ear for the treatment of SHNL.
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- Authors
- Miwa, T., Saito, H., & Akita, H.
- Affiliations
- Kyoto University (JP)
- Kumamoto University (JP)
- Chiba University (JP)
- Corresponding author
- Miwa, Toru
- Venue
- Experimental Brain Research
- Published
- 2020-11-19
- Volume
- 239
- Issue
- 2
- Pages
- 425-433
- ISSN
- 0014-4819 (ISSN-L)
- 1432-1106
- OpenAlex
- W3097986113
- Cited by
- 26
- References
- 24
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 4 · 2023: 5 · 2022: 6 · 2021: 4
- FWCI
- 1.22
- Type
- journal article
- Language
- en
- Open access
- green
- pure.teikyo.jp
- Licence
- Locations
- Experimental Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Neuroscience › Sensory Systems › Hearing, Cochlea, Tinnitus, Genetics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- lipid nanoparticles, brain-derived neurotrophic factor, mRNA delivery, round window niche, cochlea, sensorineural hearing loss, spiral ganglion neurons, neurotrophic therapy, non-invasive drug delivery, inner ear disorders, guinea pig
- MeSH terms
- Animals, Cochlea, Guinea Pigs, Lipids, RNA, Messenger, Brain-Derived Neurotrophic Factor, Nanoparticles
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