Transcriptional changes in Bifidobacterium bifidum involved in synergistic multispecies biofilms
Abstract
Bifidobacterium bifidum is part of the core microbiota of healthy infant guts where it may form biofilms on epithelial cells, mucosa, and food particles in the gut lumen. Little is known about transcriptional changes in B. bifidum engaged in synergistic multispecies biofilms with ecologically relevant species of the human gut. Recently, we reported prevalence of synergism in mixed-species biofilms formed by the human gut microbiota. This study represents a comparative gene expression analysis of B. bifidum when grown in a single-species biofilm and in two multispecies biofilm consortia with Bifidobacterium longum subsp. infantis, Bacteroides ovatus, and Parabacteroides distasonis in order to identify genes involved in this adaptive process in mixed biofilms and the influence on its metabolic and functional traits. Changes up to 58% and 43% in its genome were found when it grew in three- and four-species biofilm consortia, respectively. Upregulation of genes of B. bifidum involved in carbohydrate metabolism (particularly the galE gene), quorum sensing (luxS and pfs), and amino acid metabolism (especially branched chain amino acids) in both multispecies biofilms, compared to single-species biofilms, suggest that they may be contributing factors for the observed synergistic biofilm production when B. bifidum coexists with other species in a biofilm.
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The physiological functions of Bifidobacterium include restraining the growth of pathogenic bacteria, inhibiting proinflammatory cytokines, protecting against intestinal barrier dysfunction (in vitro and in vivo), synthesizing vitamins and amino acids in the intestine, increasing calcium bioavailability, and antitumor effects.
“The physiological functions of Bifidobacterium are mainly as follows: restraining the growth of pathogenic bacteria, inhibiting proinflammatory cytokines [26,27], protecting against intestinal barrier dysfunction (in vitro and in vivo), synthesizing vitamins and amino acids in the intestine and increasing calcium bioavailability [28,29], and antitumor effects, according to experimental results sug”
From The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties (2024) · cites this paper as [30]
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The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties
Maftei, N.-M., Raileanu, C. R., Balta, A. A., Ambrose, L., Boev, M., Marin, D. B., & Lisa, E. L. (2024) · Microorganisms · doi:10.3390/microorganisms12020234 · Cited by 381 · see citing works
- Authors
- Sadiq, F. A., Wenwei, L., Wei, C., Jianxin, Z., & Zhang, H.
- Affiliations
- Jiangnan University (CN)
- Corresponding author
- Zhang, Hao
- Venue
- Microbial Ecology
- Published
- 2021-10-21
- Volume
- 84
- Issue
- 3
- Pages
- 922-934
- ISSN
- 0095-3628 (ISSN-L)
- 1432-184X
- OpenAlex
- W3206904099
- Cited by
- 12
- References
- 63
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 2 · 2022: 3
- FWCI
- 0.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Microbial Ecology (publishedVersion)
- PubMed (publishedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (submittedVersion)
- Funders
- National Natural Science Foundation of China (31871774)
- the Fundamental Research Funds for the Central Universities (JUSRP51903B)
- the National First-class Discipline Program of Food Science and Technology (JUFSTR20180102)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- Bifidobacterium bifidum, polymicrobial biofilms, gene expression, human gut microbiome, Bifidobacterium longum subsp. infantis, Bacteroides ovatus, Parabacteroides distasonis, quorum sensing, gales, branched-chain amino acids, carbohydrate metabolism, amino acid metabolism, infant gut microbiota
- MeSH terms
- Gastrointestinal Microbiome, Bifidobacterium bifidum, Bifidobacterium, Humans, Infant, Biofilms, Microbiota
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