The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal
Open accessAbstract
Promising approaches to produce higher alcohols, e.g., isobutanol, using Escherichia coli have been developed with successful results. Here, we translated the isobutanol process from shake flasks to a 1-L bioreactor in order to characterize three E. coli strains. With in situ isobutanol removal from the bioreactor using gas stripping, the engineered E. coli strain (JCL260) produced more than 50 g/L in 72 h. In addition, the isobutanol production by the parental strain (JCL16) and the high isobutanol-tolerant mutant (SA481) were compared with JCL260. Interestingly, we found that the isobutanol-tolerant strain in fact produced worse than either JCL16 or JCL260. This result suggests that in situ product removal can properly overcome isobutanol toxicity in E. coli cultures. The isobutanol productivity was approximately twofold and the titer was 9% higher than n-butanol produced by Clostridium in a similar integrated system.
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Cites
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Self-citationEngineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production
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BackgroundMetabolic engineering of Escherichia coli for 1-butanol production
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Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli
Shen, C. R., Lan, E. I., Dekishima, Y., Baez, A., Cho, K. M., & Liao, J. C. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.03034-10 · Cited by 623
Self-citationA comprehensive metabolic map for production of bio-based chemicals
Lee, S. Y., Kim, H. U., Chae, T. U., Cho, J. S., Kim, J. W., Shin, J. H., Kim, D. I., Ko, Y.-S., Jang, W. D., & Jang, Y.-S. (2019) · Nature Catalysis · doi:10.1038/s41929-018-0212-4 · Cited by 607
A review on commercial-scale high-value products that can be produced alongside cellulosic ethanol
Rosales-Calderon, O., & Arantes, V. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1529-1 · Cited by 503
Transformation of Biomass into Commodity Chemicals Using Enzymes or Cells
Straathof, A. J. J. (2014) · Chemical Reviews · doi:10.1021/cr400309c · Cited by 459
Tao, L., Tan, E. C. D., McCormick, R., Zhang, M., Aden, A., He, X., & Zigler, B. T. (2014) · Biofuels, Bioproducts and Biorefining · doi:10.1002/bbb.1431 · Cited by 241
Metadata
- Authors
- Baez, A., Cho, K.-M., & Liao, J. C.
- Venue
- Applied Microbiology and Biotechnology
- Published
- 2011-03-10
- Volume
- 90
- Issue
- 5
- Pages
- 1681-1690
- ISSN
- 0175-7598 (ISSN-L)
- 1432-0614
- OpenAlex
- W2090813334
- Cited by
- 255
- References
- 36
- FWCI
- 7.88
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Baez, A., Cho, K.-M., & Liao, J. C. (2011). High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-011-3173-y
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