Current state of purification, isolation and analysis of bacteriocins produced by lactic acid bacteria
Abstract
The scientific interest for the search of natural means of microbial inhibitors has not faded for several years. A search of natural antibiotics, so-called bacteriocins which are produced by lactic acid bacteria (LAB), gains a huge attention of the scientists in the last century, in order to reduce the usage of synthetic food additives. Pure bacteriocins with wide spectra of antibacterial activity are promising among the natural biopreservatives. The usage of bacteriocin(s) producing LAB as starter culture for the fermentation of some food products, in order to increase their shelf-life, when synthetic preservatives are not allowable, is also possible. There are a lot of studies focusing on the isolation of new bacteriocins from traditional fermented food, dairy products and other foods or sometimes even from unusual non-food matrices. Bacteriocins producing bacteria have been isolated from different sources with the different antibacterial activity against food-borne microorganisms. This review covers the classification of bacteriocins, diversity of sources of bacteriocin(s) producing LAB, antibacterial spectra of isolated bacteriocins and analytical methods for the bacteriocin purification and analysis within the last 15 years.
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Bacteriocins are peptides with antimicrobial activity, synthesized by bacteria in ribosomes; these peptides often exhibit a narrow inhibitory spectrum and inhibit taxonomically-close bacteria.
“Bacteriocins are peptides with antimicrobial activity, synthesized by bacteria in ribosomes. These peptides often exhibit a narrow inhibitory spectrum and inhibit taxonomically-close bacteria [143,183,184,185].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [185]
Nisin A and pediocin are the only bacteriocins commercially available at present.
“The only bacteriocins commercially available at present are nisin A, produced by Lc. lactis, and pediocin, produced by P. acidilactici [51,185].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [185]
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- Authors
- Kaškonienė, V., Stankevičius, M., Bimbiraitė-Survilienė, K., Naujokaitytė, G., Šernienė, L., Mulkytė, K., Malakauskas, M., & Maruška, A.
- Affiliations
- Vytautas Magnus University (LT)
- Lithuanian University of Health Sciences (LT)
- Corresponding author
- Kaškonienė, Vilma
- Venue
- Applied Microbiology and Biotechnology
- Published
- 2017-01-09
- Volume
- 101
- Issue
- 4
- Pages
- 1323-1335
- ISSN
- 0175-7598 (ISSN-L)
- 1432-0614
- OpenAlex
- W2570650478
- Cited by
- 99
- References
- 126
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 15 · 2023: 8 · 2022: 12 · 2021: 20 · 2020: 9 · 2019: 5 · 2018: 15 · 2017: 7
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Applied Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Lithuanian University of Health Sciences (submittedVersion)
- Vytautas Magnus University (submittedVersion)
- Funders
- Research Council of Lithuania (MIP-063/2015 (BIOLACTIS))
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Keywords
- bacteriocins, lactic acid bacteria, bacteriocin purification, antibacterial activity, biopreservatives, starter cultures, fermented foods, dairy products, foodborne pathogens, natural antimicrobials, bacteriocin classification
- MeSH terms
- Bacteria, Bacteriocins, Food Microbiology, Lactic Acid
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