Trehalose-Producing Enzymes MTSase and MTHase in Anabaena 7120 Under NaCl Stress
Abstract
Salt tolerance, a multigenic trait, necessitates knowledge about biosynthesis and function of candidate gene(s) at the cellular level. Among the osmolytes, trehalose biosynthesis in cyanobacteria facing NaCl stress is little understood. Anabaena 7120 filaments exposed to 150 mM: NaCl fragmented and recovered on transfer to -NaCl medium with the increased heterocysts frequency (7%) over the control (4%). Cells failed to retain Na+ beyond a threshold [2.19 mM/cm3 (PCV)]. Whereas NaCl-stressed cells exhibited a marginal rise in K+ (1.1-fold) only at 30 h, for Na+ it was 130-fold at 48 h over cells in control. A time-course study (0-54 h) revealed reduction in intracellular Na+ beyond 48 h [0.80 mM/cm3 (PCV)] suggestive of ion efflux. The NaCl-stressed cells showed differential expression of maltooligosyltrehalose synthase (MTSase; EC 5.4.99.15) and maltooligosyltrehalose trehalohydrolase (MTHase; EC 3.2.1.141) depending on the time and the extent of intracellular Na+ buildup.
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Gene disruption experiments indicated that trehalose, like sucrose, plays a crucial role in dehydration and salt stress tolerance in different organisms.
“Gene disruption experiments indicated that trehalose, like sucrose, plays a crucial role in dehydration (Asthana et al. 2008; Higo et al. 2006) and salt stress tolerance (Salerno et al. 2004) in different organisms.”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Asthana et al. 2008
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Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering
Quintana, N., Van der Kooy, F., Van de Rhee, M. D., Voshol, G. P., & Verpoorte, R. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3394-0 · Cited by 338 · see citing works
- Authors
- Asthana, R. K., Nigam, S., Maurya, A., Kayastha, A. M., & Singh, S. P.
- Affiliations
- Banaras Hindu University (IN)
- Corresponding author
- Asthana, Ravi K.
- Venue
- Current Microbiology
- Published
- 2008-03-06
- Volume
- 56
- Issue
- 5
- Pages
- 429-435
- ISSN
- 0343-8651 (ISSN-L)
- 1432-0991
- OpenAlex
- W2001397836
- Cited by
- 15
- References
- 37
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2018: 3 · 2013: 2 · 2012: 1
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Current Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- trehalose biosynthesis, Anabaena sp. PCC 7120, cyanobacteria, NaCl stress, salt tolerance, osmolytes, intracellular sodium accumulation, ion efflux, heterocyst formation, gene expression, osmotic stress
- MeSH terms
- Superoxide Dismutase-1, Chlorates, Glucosidases, Glucosyltransferases, Potassium, Superoxide Dismutase, Trehalose, Anabaena
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