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Cited by 87Plant Stress Responses and ToleranceBacterial Genetics and BiotechnologyBiocrusts and Microbial Ecology

The role of a gene cluster for trehalose metabolism in dehydration tolerance of the filamentous cyanobacterium Anabaena sp. PCC 7120

Higo, Akiyoshi, Katoh, Hiroshi, Ohmori, Kazuko, Ikeuchi, Masahiko, Ohmori, Masayuki·2006·Microbiology
DOI 10.1099/mic.0.28583-0
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Abstract

Expression of the genes for trehalose synthesis ( mts and mth , encoding maltooligosyl trehalose synthase and hydrolase) and trehalose hydrolysis ( treH ) in Anabaena sp. PCC 7120 was up-regulated markedly upon dehydration. However, the amount of trehalose accumulated during dehydration was small, whereas a large amount of sucrose was accumulated. Northern blotting analysis revealed that these genes were transcribed as an operon. Gene disruption of mth resulted in a decrease in the trehalose level and in tolerance during dehydration. In contrast, gene disruption of treH resulted in an increase in both the amount of trehalose and tolerance. These results suggest that trehalose is important for the dehydration tolerance of this cyanobacterium. The amount of trehalose accumulated during dehydration was small, corresponding to 0·05–0·1 % of dry weight, suggesting that trehalose did not stabilize proteins and membranes directly during dehydration. To reveal the role of trehalose, the expression profiles of the wild-type strain and gene disruptants during dehydration were compared by using oligomeric DNA microarray. It was found that the expression of two genes, one of which encodes a cofactor of a chaperone DnaK, correlated with trehalose content, suggesting that a chaperone system induced by trehalose is important for the dehydration tolerance of Anabaena sp. PCC 7120.

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  • Gene disruption experiments indicated that trehalose, like sucrose, plays a crucial role in dehydration and salt stress tolerance in different organisms.

    “Gene disruption experiments indicated that trehalose, like sucrose, plays a crucial role in dehydration (Asthana et al. 2008; Higo et al. 2006) and salt stress tolerance (Salerno et al. 2004) in different organisms.”

    From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Higo et al. 2006

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Authors
Higo, A., Katoh, H., Ohmori, K., Ikeuchi, M., & Ohmori, M.
Affiliations
  • Saitama University (JP)
  • Mie University (JP)
  • Showa Women's University (JP)
  • The University of Tokyo (JP)
ORCID
Venue
Microbiology
Publisher
Microbiology Society
Published
2006-04-01
Volume
152
Issue
4
Pages
979-987
ISSN
  • 1350-0872 (ISSN-L)
  • 1465-2080
DOI
10.1099/mic.0.28583-0
OpenAlex
W2114973135
Cited by
87
References
53
Citations by year
2026: 5 · 2025: 5 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 8 · 2019: 1 · 2018: 4 · 2017: 2 · 2016: 4 · 2015: 5 · 2014: 4 · 2013: 6 · 2012: 10
FWCI
6.14
Type
journal article
Language
en
Open access
closed
Locations
  • Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Biocrusts and Microbial Ecology
Keywords
trehalose metabolism, dehydration tolerance, Anabaena sp. PCC 7120, cyanobacteria, gene clusters, operon, trehalose, sucrose accumulation, gene disruption, gene expression profiling, osmoprotection
MeSH terms
Adaptation, Physiological, Bacterial Proteins, Dehydration, Genes, Bacterial, Multigene Family, Glucosidases, Glucosyltransferases, RNA, Bacterial, RNA, Messenger, Sucrose, Transcription, Genetic, Trehalose, Blotting, Northern, Gene Expression Regulation, Bacterial, Mutagenesis, Insertional, Anabaena, Gene Deletion, Molecular Chaperones, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling

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