Complete Genomic Sequence of the Filamentous Nitrogen-fixing Cyanobacterium Anabaena sp. Strain PCC 7120
Open accessAbstract
The nucleotide sequence of the entire genome of a filamentous cyanobacterium, Anabaena sp. strain PCC 7120, was determined. The genome of Anabaena consisted of a single chromosome (6,413,771 bp) and six plasmids, designated pCC7120alpha (408,101 bp), pCC7120beta (186,614 bp), pCC7120gamma (101,965 bp), pCC7120delta (55,414 bp), pCC7120epsilon (40,340 bp), and pCC7120zeta (5,584 bp). The chromosome bears 5368 potential protein-encoding genes, four sets of rRNA genes, 48 tRNA genes representing 42 tRNA species, and 4 genes for small structural RNAs. The predicted products of 45% of the potential protein-encoding genes showed sequence similarity to known and predicted proteins of known function, and 27% to translated products of hypothetical genes. The remaining 28% lacked significant similarity to genes for known and predicted proteins in the public DNA databases. More than 60 genes involved in various processes of heterocyst formation and nitrogen fixation were assigned to the chromosome based on their similarity to the reported genes. One hundred and ninety-five genes coding for components of two-component signal transduction systems, nearly 2.5 times as many as those in Synechocystis sp. PCC 6803, were identified on the chromosome. Only 37% of the Anabaena genes showed significant sequence similarity to those of Synechocystis, indicating a high degree of divergence of the gene information between the two cyanobacterial strains.
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The genome of Anabaena sp. PCC 7120 has been entirely sequenced, demonstrating a relatively large and intricate genetic composition in comparison to many unicellular cyanobacteria.
“The genome of Anabaena sp. PCC 7120 has been entirely sequenced, demonstrating a relatively large and intricate genetic composition in comparison to many unicellular cyanobacteria [72].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [72]
Section 3.1 reports that the entire Anabaena genome was sequenced and totals 7,211,789 bp. Section 3.5.1 compares its 5,368 potential protein-coding genes with 3,167 in the unicellular Synechocystis, supporting a larger and distinct genetic complement. The paper does not establish this comparison for many unicellular cyanobacteria. (LLM, conf=0.86)
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“The genome of Anabaena sp. PCC 7120 has been entirely sequenced, demonstrating a relatively large and intricate genetic composition in comparison to many unicellular cyanobacteria [72].”
- Authors
- Kaneko, T.
- Affiliations
- Kazusa DNA Research Institute (JP)
- Corresponding author
- Kaneko, T.
- Venue
- DNA Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-01-01
- Volume
- 8
- Issue
- 5
- Pages
- 205-213
- ISSN
- 1340-2838 (ISSN-L)
- OpenAlex
- W2099553504
- Cited by
- 696
- References
- 43
- Citations by year
- 2026: 10 · 2025: 10 · 2024: 7 · 2023: 3 · 2022: 10 · 2021: 12 · 2020: 20 · 2019: 12 · 2018: 19 · 2017: 13 · 2016: 18 · 2015: 29 · 2014: 28 · 2013: 20 · 2012: 38
- FWCI
- 20.02
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- DNA Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Energy (FG0291ER20021)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Legume Nitrogen Fixing Symbiosis
- Keywords
- Anabaena sp. PCC 7120, cyanobacterial genome, nitrogen fixation, heterocyst formation, plasmids, two-component systems, Synechocystis sp. PCC 6803, gene divergence, protein-coding genes, ribosomal DNA, tRNA genes
- MeSH terms
- Genes, Bacterial, Molecular Sequence Data, Nitrogen Fixation, Plasmids, Genome, Bacterial, Anabaena, Sequence Analysis, DNA
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