Evaluation of the Decreased Cholesterol Potential of Levilactobacillus brevis M-10 Isolated from Spontaneously Fermented Sour Porridge in Mice with High-Cholesterol Levels
Abstract
Excessive cholesterol levels can lead to hypercholesterolemia, which is related to cardiovascular diseases (CVDs), and CVDs are a serious threat to human health. Therefore, lowering cholesterol levels is necessary, and diet intervention is safer than drugs are. The cholesterol-lowering effect of Levilactobacillus brevis M-10 isolated from spontaneously fermented millet sour porridge was investigated in fifty C57BL/6N male mice. After a 4-week intervention, the food intake, weight gains and organ indices were calculated; the lipid contents in the serum, liver, and feces were determined; the histopathology of the liver tissues was observed; the expression of metabolism-related genes was determined; and short-chain fatty acid (SCFA) levels in the droppings were monitored. The results showed that administration of a high dose of L. brevis M-10 (1 × 10 10 CFU/mL) significantly reduced food intake, suppressed weight gain; prevented excessive liver growth; and reduced the total serum cholesterol, triglycerides, low-density lipoproteins; and total hepatic cholesterol and triglyceride contents (P < 0.05) in high-cholesterol mice. Moreover, a high dose of L. brevis M-10 significantly promoted the fecal excretion of cholesterol and triglycerides (P < 0.05) and alleviated liver damage induced by a high-cholesterol diet. Furthermore, a high dose of L. brevis M-10 significantly downregulated the cholesterol metabolism-related gene expression of NPC1L1, ACAT2, HMG-CoA, and SREBP2 but upregulated the gene expression of ABCG5, CYP7A1, and LXR-α (P < 0.05). Additionally, a high dose of L. brevis M-10 significantly increased SCFA contents, including those of acetic acid, propionic acid and n-butyric acid (P < 0.05). These findings could provide support for the use of L. brevis M-10 in the application of functional foods to alleviate hypercholesterolemia.
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L. brevis bile tolerance and cholesterol-lowering activity in vitro are consistent with multiple bsh gene copies.
“Genomic analyses indicate that L. brevis carries multiple bsh gene copies of different subtypes [9], consistent with observed bile tolerance and cholesterol‐lowering activity in vitro [28–30].”
From Bile Salt Hydrolase Activity in the Food-Derived Strain Levilactobacillus brevis M3R3: Genomic and Functional Characterization (2026) · cites this paper as [28]
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- Authors
- Wang, Q., Peng, J., Tian, Y., Li, J., Cai, J., & Qin, W.
- Affiliations
- Shanxi University (CN)
- Shanxi Provincial People’s Hospital (CN)
- China Institute for Radiation Protection (CN)
- Corresponding author
- Qin, Wenjun
- Venue
- Current Microbiology
- Published
- 2024-11-30
- Volume
- 82
- Issue
- 1
- Pages
- 24-24
- ISSN
- 0343-8651 (ISSN-L)
- 1432-0991
- OpenAlex
- W4404862095
- Cited by
- 5
- References
- 39
- Citations by year
- 2026: 2 · 2025: 3
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Current Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Natural Science Foundation of Shanxi Province (20210302123464)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- Lactobacillus brevis, hypercholesterolemia, cholesterol-lowering effect, probiotics, high-cholesterol diet, hepatic cholesterol metabolism, short-chain fatty acids, fecal cholesterol excretion, liver histopathology, NPC1L1, SREBP2, ABCG5, CYP7A1, liver X receptor alpha, ACAT2, functional foods, cardiovascular disease prevention
- MeSH terms
- Fermented Foods, Animals, Anticholesteremic Agents, Cholesterol, Fatty Acids, Volatile, Feces, Fermentation, Hypercholesterolemia, Liver, Male, Mice, Inbred C57BL, Triglycerides, Probiotics, Mice, Levilactobacillus brevis
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