Cited by 40Probiotics and Fermented FoodsGut microbiota and healthAnimal Nutrition and Physiology

Levilactobacillus brevis from carnivores can ameliorate hypercholesterolemia: In vitro and in vivo mechanistic evidence

Munir, Aneela, Javed, Ghulam Ayesha, Javed, Saman, Arshad, Najma·2022·Journal of Applied Microbiology

Abstract

Aims: The aim was to explore the probiotic and hypocholesterolaemic potential of two Levilactobacillus brevis strains of carnivore origin along with selected underlying mechanisms. Methods and Results: Levilactobacillus brevis MT950194 and L. brevis MW365351 were analysed in vitro for oro-gastro-intestinal stress tolerance, cholesterol reduction, cholesterol adsorption (through scanning electron microscopy) and bile salt hydrolase (BSH) activity. Strains could survive (>80%) in oro-gastro-intestinal conditions and reduce high amount of cholesterol (35% and 54%) from media containing bile salts (0.3%) as compared with Lactobacillus acidophilus ATCC 4356 and presented the least pathogenicity towards mammalian cells. Exopolysaccharide production, cell surface cholesterol adherence and BSH activity were witnessed as possible cholesterol-lowering mechanisms. In in vivo experiment, the treatments of hypercholesterolaemic rats with L. brevis MT950194, L. brevis MW365351 and their mixture led to significant (p < 0.05) reduction in serum and hepatic cholesterol, low-density lipids, cholesterol ratio, liver steatosis and size of adipocytes. It further ameliorated diet-induced changes in hepatic enzymes. Conclusions: Levilactobacillus brevis MT950194 and L. brevis MW365351 from carnivores have probiotic pharmacological potential and can reduce serum cholesterol through surface adherence and BSH production. Significance and Impact of the Study: These strains may be utilized in treating hypercholesterolaemia and production of low-fat functional foods.

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Crossref · 10.1111/jam.15678

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Authors
Munir, A., Javed, G. A., Javed, S., & Arshad, N.
Affiliations
  • University of the Punjab (PK)
  • University of Lahore (PK)
Venue
Journal of Applied Microbiology
Published
2022-09-01
Volume
133
Issue
3
Pages
1725-1742
ISSN
  • 1364-5072 (ISSN-L)
  • 1365-2672
OpenAlex
W4283315615
Cited by
40
References
80
Citations by year
2026: 11 · 2025: 20 · 2024: 4 · 2023: 4 · 2022: 1
FWCI
3.86
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Applied Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Keywords
Lactobacillus brevis, probiotics, hypercholesterolemia, bile salt hydrolase, cholesterol adsorption, exopolysaccharide production, liver cholesterol, hepatic steatosis, adipocyte size, functional foods
MeSH terms
Animals, Bile Acids and Salts, Cholesterol, Hypercholesterolemia, Lactobacillaceae, Mammals, Probiotics, Rats, Levilactobacillus brevis

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