Characterization of gut microbiota profiles in coronary artery disease patients using data mining analysis of terminal restriction fragment length polymorphism: gut microbiota could be a diagnostic marker of coronary artery disease
Abstract
The association between atherosclerosis and gut microbiota has been attracting increased attention. We previously demonstrated a possible link between gut microbiota and coronary artery disease. Our aim of this study was to clarify the gut microbiota profiles in coronary artery disease patients using data mining analysis of terminal restriction fragment length polymorphism (T-RFLP). This study included 39 coronary artery disease (CAD) patients and 30 age- and sex- matched no-CAD controls (Ctrls) with coronary risk factors. Bacterial DNA was extracted from their fecal samples and analyzed by T-RFLP and data mining analysis using the classification and regression algorithm. Five additional CAD patients were newly recruited to confirm the reliability of this analysis. Data mining analysis could divide the composition of gut microbiota into 2 characteristic nodes. The CAD group was classified into 4 CAD pattern nodes (35/39 = 90 %), while the Ctrl group was classified into 3 Ctrl pattern nodes (28/30 = 93 %). Five additional CAD samples were applied to the same dividing model, which could validate the accuracy to predict the risk of CAD by data mining analysis. We could demonstrate that operational taxonomic unit 853 (OTU853), OTU657, and OTU990 were determined important both by the data mining method and by the usual statistical comparison. We classified the gut microbiota profiles in coronary artery disease patients using data mining analysis of T-RFLP data and demonstrated the possibility that gut microbiota is a diagnostic marker of suffering from CAD.
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Gut microbiota has a strong relationship with the progression of cardiovascular diseases, including coronary artery disease.
“Increasing evidence has suggested a strong relationship between gut microbiota and the progression of cardiovascular diseases, and we previously reported such a relationship with coronary artery disease (CAD) for the first time.1,2”
From <i>Bacteroides vulgatus</i> and <i>Bacteroides dorei</i> Reduce Gut Microbial Lipopolysaccharide Production and Inhibit Atherosclerosis (2018) · cites this paper as [2]
The abundance of Bacteroides is lower in coronary artery disease patients than in non-CAD patients with coronary risk factors or in healthy volunteers.
“our previous study using terminal-restriction fragment length polymorphism analysis confirmed that the abundance of Bacteroides is lower in CAD patients than in non-CAD patients with coronary risk factors or in healthy volunteers.1,2”
From <i>Bacteroides vulgatus</i> and <i>Bacteroides dorei</i> Reduce Gut Microbial Lipopolysaccharide Production and Inhibit Atherosclerosis (2018) · cites this paper as [2]
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- Authors
- Emoto, T., Yamashita, T., Kobayashi, T., Sasaki, N., Hirota, Y., Hayashi, T., So, A., Kasahara, K., Yodoi, K., Matsumoto, T., Mizoguchi, T., Ogawa, W., & Hirata, K.-I.
- Affiliations
- Kobe University (JP)
- Miyagi University (JP)
- Corresponding author
- Yamashita, Tomoya
- ORCID
- Venue
- Heart and Vessels
- Published
- 2016-04-28
- Volume
- 32
- Issue
- 1
- Pages
- 39-46
- ISSN
- 0910-8327 (ISSN-L)
- 1615-2573
- OpenAlex
- W2344361464
- Cited by
- 174
- References
- 32
- Citations by year
- 2026: 6 · 2025: 11 · 2024: 20 · 2023: 21 · 2022: 33 · 2021: 25 · 2020: 22 · 2019: 13 · 2018: 13 · 2017: 6 · 2016: 4
- FWCI
- 6.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Heart and Vessels (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Takeda Science Foundation
- Suzuken Memorial Foundation
- Uehara Memorial Foundation
- Mochida Memorial Foundation for Medical and Pharmaceutical Research
- Mitsui Life Social Welfare Foundation
- Kanae Medical Foundation
- Senshin Medical Research Foundation
- Yakult Bioscience Research Foundation
- Translational Research Grant from Japan Circulation Society
- Arteriosclerosis Research Foundation of Japan
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Keywords
- gut microbiota, coronary artery disease, atherosclerosis, diagnostic biomarkers, fecal microbiota, T-RFLP, data mining classification, operational taxonomic units, microbiota profiling
- MeSH terms
- Gastrointestinal Microbiome, Aged, Coronary Artery Disease, DNA, Bacterial, Feces, Female, Humans, Japan, Male, Middle Aged, Polymorphism, Restriction Fragment Length, Biomarkers, Case-Control Studies, Data Mining
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