The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
An updated histological classification system for multiple sclerosis lesions
Abstract
Multiple sclerosis is a complex and heterogeneous, most likely autoimmune, demyelinating disease of the central nervous system (CNS). Although a number of histological classification systems for CNS lesions have been used by different groups in recent years, no uniform classification exists. In this paper, we propose a simple and unifying classification of MS lesions incorporating many elements of earlier histological systems that aims to provide guidelines for neuropathologists and researchers studying MS lesions to allow for better comparison of different studies performed with MS tissue, and to aid in understanding the pathogenesis of the disease. Based on the presence/absence and distribution of macrophages/microglia (inflammatory activity) and the presence/absence of ongoing demyelination (demyelinating activity), we suggest differentiating between active, mixed active/inactive, and inactive lesions with or without ongoing demyelination. Active lesions are characterized by macrophages/microglia throughout the lesion area, whereas mixed active/inactive lesions have a hypocellular lesion center with macrophages/microglia limited to the lesion border. Inactive lesions are almost completely lacking macrophages/microglia. Active and mixed active/inactive lesions can be further subdivided into lesions with ongoing myelin destruction (demyelinating lesions) and lesions in which the destruction of myelin has ceased, but macrophages are still present (post-demyelinating lesions). This distinction is based on the presence or absence of myelin degradation products within the cytoplasm of macrophages/microglia. For this classification of MS lesions, identification of myelin with histological stains [such as luxol fast blue-PAS] or by immunohistochemistry using antibodies against myelin basic-protein (MBP) or proteolipid-protein (PLP), as well as, detection of macrophages/microglia by, e.g., anti-CD68 is sufficient. Active and demyelinating lesions may be further subdivided into the early and late demyelinating lesions. The former is defined by the presence in macrophages of major and small molecular weight myelin proteins, such as cyclic nucleotide diphosphoesterase (CNP), myelin oligodendrocyte glycoprotein (MOG), or myelin-associated protein (MAG), whereas macrophages in the latter demonstrate merely the presence of the major myelin proteins MBP or PLP. We discuss the histological features and staining techniques required to classify MS lesions, and, in addition, describe the histological hallmarks of cortical pathology and diffuse white matter changes, as well as of remyelination.
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“Monocytes are recruited to plaques in MS [193] (Fig. 1j-l) and have long been considered to play a pathological role in EAE.”
From Microglia and monocytes in inflammatory CNS disease: integrating phenotype and function (2022) · cites this paper as [193]
In the citing paper
“Neuroinflammation is present in all stages of MS, and a proposed classification of MS lesions relies in part on the presence or absence of microglia in the lesions66.”
From Microglia in neurodegeneration (2018) · cites this paper as [66]
Citations
Cites
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Self-citation
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Sorted by citations
Microglia in neurodegeneration
Hickman, S., Izzy, S., Sen, P., Morsett, L., & El Khoury, J. (2018) · Nature Neuroscience · doi:10.1038/s41593-018-0242-x · Cited by 1,710
A lymphocyte–microglia–astrocyte axis in chronic active multiple sclerosis
Absinta, M., Maric, D., Gharagozloo, M., Garton, T., Smith, M. D., Jin, J., Fitzgerald, K. C., Song, A., Liu, P., Lin, J.-P., et al. (2021) · Nature · doi:10.1038/s41586-021-03892-7 · Cited by 768
Myelin in the Central Nervous System: Structure, Function, and Pathology
Stadelmann, C., Timmler, S., Barrantes‐Freer, A., & Simons, M. (2019) · Physiological Reviews · doi:10.1152/physrev.00031.2018 · Cited by 799
Pathogenic Mechanisms Associated With Different Clinical Courses of Multiple Sclerosis
Lassmann, H. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2018.03116 · Cited by 711
Self-citationAssessment of lesions on magnetic resonance imaging in multiple sclerosis: practical guidelines
Filippi, M., Preziosa, P., Banwell, B., Barkhof, F., Ciccarelli, O., Stefano, N. D., Geurts, J. J. G., Paul, F., Reich, D. S., Toosy, A., et al. (2019) · Brain · doi:10.1093/brain/awz144 · Cited by 607
Metadata
- Authors
- Kuhlmann, T., Ludwin, S., Prat, A., Antel, J., Brück, W., & Lassmann, H.
- Affiliations
- University Hospital Münster (DE)
- Montreal Neurological Institute and Hospital (CA)
- Centre Hospitalier de l’Université de Montréal (CA)
- Universitätsmedizin Göttingen (DE)
- Medical University of Vienna (AT)
- Corresponding author
- Kuhlmann, Tanja
- Venue
- Acta Neuropathologica
- Published
- 2016-12-17
- Volume
- 133
- Issue
- 1
- Pages
- 13-24
- ISSN
- 0001-6322 (ISSN-L)
- 1432-0533
- OpenAlex
- W2562977489
- Cited by
- 692
- References
- 59
- Citations by year
- 2026: 58 · 2025: 87 · 2024: 113 · 2023: 81 · 2022: 99 · 2021: 83 · 2020: 59 · 2019: 51 · 2018: 42 · 2017: 17
- FWCI
- 33.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Neuropathologica (publishedVersion)
- PubMed (publishedVersion)
- GRO (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (SFB-TRR128 B-7, Ku1477/6-1)
- IZKF (KuT3/012/15)
- Austrian Science Fund (FWF Project I 2114-B27)
- CIHR; Quebec, FRQS
- CIHR, Quebec, FRQS
- BMBF, KKNMS
- Topics
- Health Sciences › Medicine › Clinical Neurology › Multiple Sclerosis Research Studies
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Clinical Neurology › Peripheral Neuropathies and Disorders
- Keywords
- multiple sclerosis, histological classification, demyelination, macrophages, microglia, active lesions, myelin basic protein, proteolipid protein, myelin oligodendrocyte glycoprotein, myelin-associated glycoprotein, cortical pathology, remyelination, demyelinating disease, central nervous system lesions, neuropathology
- MeSH terms
- Animals, Humans, Macrophages, Multiple Sclerosis, Microglia, White Matter
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