Multiple interactions between cryptochrome and phototropin blue-light signalling pathways in Arabidopsis thaliana
Abstract
Higher plants contain two structurally unrelated flavoprotein blue-light photoreceptors, the cryptochromes and the phototropins, which mediate largely distinct response pathways. Cryptochromes regulate plant development and photomorphogenesis whereas phototropins are primarily implicated in photomovement responses such as phototropism and chloroplast relocation. In the present study we identify interactions between cryptochromes and phototropins in several photoresponses of Arabidopsis thaliana. Cryptochromes are shown to exert a positive effect on phototropic curvature under long-term irradiation conditions. Specifically, in a phot1-deficient genetic background (phot1 mutant), curvature is reduced in the absence of cryptochromes, particularly at wavelengths where cryptochromes show preferential absorption. Phototropins in turn exert a small promotive effect on such cryptochrome-mediated responses as hypocotyl elongation and anthocyanin accumulation. These effects are apparent in a cryptochrome-deficient (cry1cry2 mutant) genetic background. In addition to positive interactions between signalling pathways, we demonstrate that the cryptochromes also exert a negative regulatory effect. Levels of phot1 protein decrease in blue light as a function of cryptochrome photoreceptor activation. This negative regulation occurs in part at the level of phot1 transcription but may also involve post-transcriptional mechanisms. These two classes of photoreceptor thereby reciprocally modulate their overall responsivity to blue light through multiple forms of interaction.
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Phytochrome A and cryptochromes sense blue light signals, are localized in the nucleus, and indirectly affect hypocotyl phototropism by regulating transcription of related genes.
“Phytochrome A (PHYA) and cryptochromes (CRYs) can also sense blue light signals. These proteins are localized in the nucleus and indirectly affect the phototropism of hypocotyls by regulating the transcription of related genes (Kang et al., 2008; Nagashima et al., 2008b; Goyal et al., 2013; Liscum et al., 2014).”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Kang et al., 2008
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Phototropism: Growing towards an Understanding of Plant Movement
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The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants
Xin, G.-Y., Li, L.-P., Wang, P.-T., Li, X.-Y., Han, Y.-J., & Zhao, X. (2022) · Stress Biology · doi:10.1007/s44154-022-00066-x · Cited by 17 · see citing works
Suzuki, H., Okamoto, A., Kojima, A., Nishimura, T., Takano, M., Kagawa, T., Kadota, A., Kanegae, T., & Koshiba, T. (2014) · Planta · doi:10.1007/s00425-014-2082-6 · Cited by 12 · see citing works
- Authors
- Kang, B., Grancher, N., Koyffmann, V., Lardemer, D., Burney, S., & Ahmad, M.
- Affiliations
- Pennsylvania State University (US)
- Université Pierre-et-Marie-Curie (FR)
- Corresponding author
- Ahmad, Margaret
- ORCID
- Ahmad, Margaret
- Venue
- Planta
- Published
- 2008-01-09
- Volume
- 227
- Issue
- 5
- Pages
- 1091-1099
- ISSN
- 0032-0935 (ISSN-L)
- 1432-2048
- OpenAlex
- W1987284502
- Cited by
- 57
- References
- 30
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 4 · 2020: 2 · 2019: 3 · 2018: 4 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 8
- FWCI
- 5.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Planta (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Keywords
- cryptochrome, phototropin, phototropism, photomorphogenesis, hypocotyl elongation, anthocyanin accumulation, post-transcriptional regulation, Arabidopsis thaliana
- MeSH terms
- Anthocyanins, Flavoproteins, Light, Mutation, Blotting, Western, Signal Transduction, Arabidopsis, Phototropism, Hypocotyl, Reverse Transcriptase Polymerase Chain Reaction, Arabidopsis Proteins, Cryptochromes
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