Blue-light regulation of ZmPHOT1 and ZmPHOT2 gene expression and the possible involvement of Zmphot1 in phototropism in maize coleoptiles
Abstract
Main conclusion ZmPHOT1 and ZmPHOT2 are expressed differentially in maize coleoptiles and leaves, with Zmphot1 possibly involved in first-positive phototropic curvature of red-light-adapted maize coleoptiles exposed to pulsed low-fluence blue light. Unilateral blue-light perception by phototropin(s) is the first event of phototropism, with the subsequent signal causing lateral transport of auxin at the coleoptile tip region of monocots. In this study, we analyzed the behavior of two maize phototropin genes: ZmPHOT1 and ZmPHOT2, the latter identified from the maize genome database and newly characterized. Quantitative real-time PCR analysis demonstrated that ZmPHOT1 was abundantly expressed in etiolated coleoptiles, while lower expressions of both ZmPHOT1 and ZmPHOT2 were observed in young leaves. Interestingly, these genes were not specifically expressed in the coleoptile tip region, a key position for photoperception in phototropism. Exposure to pulsed low-fluence blue light (LBL) (0.33 µmol m(-2) s(-1) × 8 s) and continuous high-fluence blue light (HBL) (10 µmol m(-2) s(-1)) rapidly decreased ZmPHOT1 gene expression in coleoptiles, with levels of ZmPHOT2 not significantly altered in that tissue. In young leaves, no drastic expression changes were induced in either ZmPHOT1 or ZmPHOT2 by LBL or HBL irradiation. The Zmphot1 protein was investigated by Western blot analysis with anti-Osphot1 antibodies. Zmphot1 was detected in microsomal fractions, with higher levels in coleoptiles than in leaves. HBL caused rapid phosphorylation of the protein, whereas no phot1 phosphorylation was induced by LBL. The involvement of Zmphot1 in LBL-induced phototropic curvature of maize coleoptiles is discussed.
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Low-fluence blue light induces phosphorylation of zmphot1, and five phosphorylation sites were detected; Ser369, Ser291, and Ser376 participate in regulating the first positive phototropism but do not affect the second positive phototropism.
“Low-fluence blue light was recently shown to induce the phosphorylation of zmphot1, and five phosphorylation sites were detected (Ser265, Ser291, Ser369, Ser376 and Ser753). Among them, Ser369, Ser291, and Ser376 participate in regulating the first positive phototropism but do not affect the second positive phototropism (Suzuki et al., 2014).”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Suzuki et al., 2014
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- Authors
- Suzuki, H., Okamoto, A., Kojima, A., Nishimura, T., Takano, M., Kagawa, T., Kadota, A., Kanegae, T., & Koshiba, T.
- Affiliations
- Tokyo Metropolitan University (JP)
- Institute of Agrobiological Sciences (JP)
- Corresponding author
- Koshiba, Tomokazu
- Venue
- Planta
- Published
- 2014-05-11
- Volume
- 240
- Issue
- 2
- Pages
- 251-261
- ISSN
- 0032-0935 (ISSN-L)
- 1432-2048
- OpenAlex
- W2018982289
- Cited by
- 12
- References
- 49
- Citations by year
- 2025: 2 · 2024: 1 · 2022: 2 · 2020: 2 · 2019: 2 · 2017: 1 · 2016: 1 · 2014: 1
- FWCI
- 1.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Planta (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (23570061, 23570060)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Keywords
- phototropin, phototropism, blue light, maize coleoptiles, gene expression, auxin transport, light perception, monocotyledons
- MeSH terms
- Zea mays, Light, Phosphorylation, Plant Proteins, Blotting, Western, Plant Leaves, Phototropism, Cotyledon, Real-Time Polymerase Chain Reaction
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