Structural revision of sulfated polysaccharide B-1 isolated from a marine Pseudomonas species and its cytotoxic activity against human cancer cell lines
Abstract
The structure of a sulfated polysaccharide (B-1) isolated and purified from the culture filtrate of marine Pseudomonas sp. WAK-1 was revised to have a repeating unit as follows: -2)-beta-D-Galp(4SO4)(1-4)[beta-D-Glcp(1-6)]-beta-D-Galp(3SO4)(1-. B-1 was evaluated for anticancer activity using a human cancer cell line panel coupled with a drug sensitivity database. The average B-1 concentration required for 50% growth inhibition against the panel of 39 cell lines was 63.2 micro g/ml. Among the cancer cell lines tested, high sensitivities to B-1 were observed in central nervous system cancer and lung cancer cell lines. The COMPARE analysis revealed that the differential growth inhibition pattern of B-1 had no significant correlation with those of more than 200 standard compounds, most of which were anticancer drugs and different types of inhibitors. This lack of similarities in the cytotoxic patterns appears to reflect previously unrecognized biological properties of B-1. It was revealed that B-1 induced apoptosis in U937 cells, as shown by cell morphology and internucleosomal DNA fragmentation.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A sulfated polysaccharide isolated from marine Pseudomonas spp. (B-1) filtrate induced apoptosis of human leukaemic cells (U937).
“A sulfated polysaccharide isolated from a marine Pseudomonas spp. (B-1) filtrate induced apoptosis of human leukaemic cells (U937) [172], whereas pancreatic islet carcinoma apoptosis was observed with PI-88, a sulfated oligosaccharide [173].”
From Marine Natural Products: A Source of Novel Anticancer Drugs (2019) · cites this paper as [172]
Polysaccharide B1 from the marine Pseudomonas sp. demonstrated cytotoxicity against tumor cells, being more active to the central nervous system and lung cancer cell lines.
“Polysaccharide B1 from the marine Pseudomonas sp. has repeating units as -2)-β-d-Galp(4-sulfate)(1,4)[β-d-Glcp(1,6)]-β-d-Galp(3-sulfate)(1- and demonstrated cytotoxicity against tumor cells, being more active to the central nervous system and lung cancer cell lines.”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [126]
Polysaccharide B1 from the marine Pseudomonas sp. induced apoptosis in U937 cells.
“It induced apoptosis in U937 cells [126].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [126]
Cites
Apoptosis: A Basic Biological Phenomenon with Wideranging Implications in Tissue Kinetics
Kerr, J. F. R., Wyllie, A. H., & Currie, A. R. (1972) · British Journal of Cancer · doi:10.1038/bjc.1972.33 · Cited by 15,673 · see citing works
Paull, K. D., Shoemaker, R. H., Hodes, L., Monks, A. P., Scudiero, D. A., Rubinstein, L., Plowman, J., & Boyd, M. R. (1989) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/81.14.1088 · Cited by 970 · see citing works
Rubinstein, L., Shoemaker, R. H., Paull, K. D., Simon, R. M., Tosini, S., Skehan, P., Scudiero, D. A., Monks, A. P., & Boyd, M. R. (1990) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/82.13.1113 · Cited by 927 · see citing works
Apoptosis induced by anticancer drugs
Hickman, J. A. (1992) · Cancer and Metastasis Reviews · doi:10.1007/bf00048059 · Cited by 860 · see citing works
Programmed cell death: Apoptosis and oncogenesis
Williams, G. T. (1991) · Cell · doi:10.1016/0092-8674(91)90002-g · Cited by 845 · see citing works
Activation of Murine Macrophages by Polysaccharide Fractions from Marine Algae (Porphyra yezoensis)
Yoshizawa, Y., Enomoto, A., Todoh, H., Ametani, A., & Kaminogawa, S. (1993) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.57.1862 · Cited by 109 · see citing works
The involvement of oncogenes and tumor suppressor genes in the control of apoptosis
Oren, M. (1992) · Cancer and Metastasis Reviews · doi:10.1007/bf00048060 · Cited by 96 · see citing works
Antiviral Activities of Marine Pseudomonas Polysaccharides and Their Oversulfated Derivatives
Matsuda, M., Shigeta, S., & Okutani, K. (1999) · Marine Biotechnology · doi:10.1007/pl00011753 · Cited by 51 · see citing works
Self-citationControl of Apoptosis and Growth of Malignant T Lymphoma Cells by Lymph Node Stromal Cells
Kataoka, S., Naito, M., Fujita, N., Ishii, H., Ishii, S., Yamori, T., Nakajima, M., & TSURUO, T. (1993) · Experimental Cell Research · doi:10.1006/excr.1993.1193 · Cited by 27 · see citing works
Self-citationSimultaneous Production of Muco- and Sulfated Polysaccharides by Marine Pseudomonas.
Matsuda, M., Worawattanamateekul, W., & Okutani, K. (1992) · NIPPON SUISAN GAKKAISHI · doi:10.2331/suisan.58.1735 · Cited by 18 · see citing works
Self-citation[A human cell line panel for screening anti-cancer drugs].
Yamori, T. (1997) · PubMed · Cited by 11 · see citing works
Self-citationStructural Analysis of a Sulfated Polysaccharide from a Marine Pseudomonas.
Matsuda, M., Hasui, M., & Okutani, K. (1993) · NIPPON SUISAN GAKKAISHI · doi:10.2331/suisan.59.535 · Cited by 9 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Marine Polysaccharides in Pharmaceutical Applications: An Overview
Laurienzo, P. (2010) · Marine Drugs · doi:10.3390/md8092435 · Cited by 538 · see citing works
Marine Natural Products: A Source of Novel Anticancer Drugs
Khalifa, S. A. M., Elias, N., Farag, M. A., Chen, L., Saeed, A., Hegazy, M.-E. F., Moustafa, M. S., Abd El-Wahed, A., Al-Mousawi, S. M., Musharraf, S. G., et al. (2019) · Marine Drugs · doi:10.3390/md17090491 · Cited by 555 · see citing works
Bioactivities, isolation and purification methods of polysaccharides from natural products: A review
Shi, L. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.06.100 · Cited by 563 · see citing works
Anticancer Drugs from Marine Flora: An Overview
Boopathy, N. S., & Kathiresan, K. (2010) · Journal of Oncology · doi:10.1155/2010/214186 · Cited by 402 · see citing works
Casillo, A., Lanzetta, R., Parrilli, M., & Corsaro, M. M. (2018) · Marine Drugs · doi:10.3390/md16020069 · Cited by 251 · see citing works
Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects
Fedorov, S., Ermakova, S., Zvyagintseva, T., & Stonik, V. (2013) · Marine Drugs · doi:10.3390/md11124876 · Cited by 189 · see citing works
Marine Anticancer Agents: An Overview with a Particular Focus on Their Chemical Classes
Barreca, M., Spanò, V., Montalbano, A., Cueto, M., Díaz‐Marrero, A. R., Deniz, İ., Erdoğan, A., Bilela, L. L., Moulin, C., de Givenchy, E. T., et al. (2020) · Marine Drugs · doi:10.3390/md18120619 · Cited by 150 · see citing works
Escárcega‐González, C. E., Garza-Cervantes, J. A., Rodríguez, A. V., & Morones‐Ramírez, J. R. (2018) · International Journal of Polymer Science · doi:10.1155/2018/7045852 · Cited by 105 · see citing works
Zhang, F., Shi, J.-J., Thakur, K., Hu, F., Zhang, J., & Wei, Z. (2017) · Frontiers in Pharmacology · doi:10.3389/fphar.2017.00102 · Cited by 101 · see citing works
Athukorala, Y., Ahn, G. N., Jee, Y.-H., Kim, G.-Y., Kim, S.-H., Ha, J.-H., Kang, J.-S., Lee, K.-W., & Jeon, Y.-J. (2009) · Journal of Applied Phycology · doi:10.1007/s10811-008-9368-7 · Cited by 89 · see citing works
Bacterial polysaccharides—A big source for prebiotics and therapeutics
Khan, R., Shah, M. D., Shah, L., Lee, P.-C., & Khan, I. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.1031935 · Cited by 88 · see citing works
Bioactive substances produced by marine isolates of Pseudomonas
Isnansetyo, A., & Kamei, Y. (2009) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-009-0611-2 · Cited by 86 · see citing works
Exopolysaccharides from Marine Microbes: Source, Structure and Application
Qi, M., Zheng, C., Wu, W., Yu, G., & Wang, P. (2022) · Marine Drugs · doi:10.3390/md20080512 · Cited by 81 · see citing works
Mishra, A., & Jha, B. (2013) · Springer eBooks · doi:10.1007/978-3-642-31331-8_25 · Cited by 78 · see citing works
Gugliandolo, C., Spanò, A., Lentini, V., Arena, A., & Maugeri, T. L. (2013) · Journal of Applied Microbiology · doi:10.1111/jam.12422 · Cited by 67 · see citing works
Bilal, M., & Iqbal, H. M. N. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.02.037 · Cited by 61 · see citing works
Kamble, P., Cheriyamundath, S., Lopus, M., & Vavilala, S. L. (2018) · Journal of Applied Phycology · doi:10.1007/s10811-018-1397-2 · Cited by 55 · see citing works
Sun, M., Zhao, F., Chen, X., Zhang, X., Zhang, Y., Song, X., Sun, C., & Yang, J. (2020) · Marine Drugs · doi:10.3390/md18010048 · Cited by 55 · see citing works
Anticancer Drugs Discovery and Development from Marine Organisms
Chakraborty, C., Hsu, C., Wen, Z., & Lin, C.-S. (2009) · Current Topics in Medicinal Chemistry · doi:10.2174/156802609789909803 · Cited by 45 · see citing works
Chu, H.-L., Mao, H., Feng, W., Liu, J., & Geng, Y. (2012) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2012.12.025 · Cited by 39 · see citing works
Ko, S., Lee, S., Ahn, G., Kim, K., Cha, S., Kim, S., Jeon, B.-T., Park, P., Lee, K.-W., & Jeon, Y. (2011) · Journal of Applied Phycology · doi:10.1007/s10811-011-9685-0 · Cited by 38 · see citing works
Tahmourespour, A., Ahmadi, A., & Fesharaki, M. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.01.268 · Cited by 38 · see citing works
WU, X., Chen, D., & XIE, G. (2006) · Cell Biology International · doi:10.1016/j.cellbi.2006.04.005 · Cited by 33 · see citing works
Bioactive Carbohydrate Polymers—Between Myth and Reality
Drira, M., Hentati, F., Babich, O., Sukhikh, S., Larina, V., Sharifian, S., Homaei, A. A., Fendri, I., Lemos, M. F. L., Félix, C., et al. (2021) · Molecules · doi:10.3390/molecules26237068 · Cited by 34 · see citing works
Zayed, A., Mansour, M. K., Sedeek, M. S., Habib, M. H., Ulber, R., & Farag, M. A. (2021) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2021.1942779 · Cited by 32 · see citing works
Exopolysaccharides from marine bacteria: production, recovery and applications
Dave, S. R., Upadhyay, K. H., Vaishnav, A. M., & Tipre, D. R. (2020) · Environmental Sustainability · doi:10.1007/s42398-020-00101-5 · Cited by 31 · see citing works
Campa‐Córdova, Á. I., Núñez-Vázquez, E. J., Luna‐González, A., Romero‐Geraldo, M. D. J., & Ascencio, F. (2008) · Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology · doi:10.1016/j.cbpc.2008.08.006 · Cited by 28 · see citing works
Medical application of exopolymers produced by marine bacteria
Abdelhamid, S. A., Mohamed, S. S., & Selim, M. S. (2020) · Bulletin of the National Research Centre/Bulletin of the National Research Center · doi:10.1186/s42269-020-00323-x · Cited by 28 · see citing works
Campa-Córdova, A., Hernández-Saavedra, N. Y., Aguirre-Guzmán, G., & Ascencio, F. (2005) · Ciencias Marinas · doi:10.7773/cm.v31i4.32 · Cited by 25 · see citing works
Das, M. K., Sahu, P. K., Rao, G. S., Mukkanti, K., & Silpavathi, L. (2014) · Saudi Journal of Biological Sciences · doi:10.1016/j.sjbs.2014.02.003 · Cited by 23 · see citing works
Marine bacterial extracellular polysaccharides: A review
(2016) · Journal of Coastal Life Medicine · doi:10.12980/jclm.5.2017j6-207 · Cited by 23 · see citing works
Zhan, T., Bai, Q., & Zhao, Z. (2021) · Arabian Journal of Chemistry · doi:10.1016/j.arabjc.2021.103389 · Cited by 22 · see citing works
Effects of sulfated polysaccharides on tumour biology
Wu, X., & Chen, D. (2006) · West Indian Medical Journal · doi:10.1590/s0043-31442006000400009 · Cited by 22 · see citing works
Microbial Exopolysaccharides: Structure and Therapeutic Properties
Santra, H. K., & Banerjee, D. (2021) · Springer eBooks · doi:10.1007/978-981-16-0045-6_17 · Cited by 20 · see citing works
The sulfated O-specific polysaccharide from the marine bacterium Cobetia pacifica KMM 3879T
Kokoulin, M. S., Kalinovsky, A. I., Komandrova, N. A., Tomshich, S. V., Romanenko, L. A., & Vaskovsky, V. E. (2014) · Carbohydrate Research · doi:10.1016/j.carres.2014.01.015 · Cited by 18 · see citing works
Heparin-like Entities from Marine Organisms
Colliec‐Jouault, S., Bavington, C. D., & Delbarre‐Ladrat, C. (2011) · Handbook of experimental pharmacology · doi:10.1007/978-3-642-23056-1_19 · Cited by 17 · see citing works
Kokoulin, M. S., Kuzmich, A. S., Filshtein, A. P., Prassolov, V., & Romanenko, L. A. (2024) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2024.122791 · Cited by 15 · see citing works
A review on marine source as anticancer agents
Rai, T., Kaushik, N., Malviya, R., & Sharma, P. K. (2023) · Journal of Asian Natural Products Research · doi:10.1080/10286020.2023.2249825 · Cited by 14 · see citing works
Pseudomonas for Industrial Biotechnology
Kahlon, R. S. (2016) · Springer eBooks · doi:10.1007/978-3-319-31198-2_8 · Cited by 8 · see citing works
Marine-Derived Polysaccharides: Prospects for Future Pharmaceuticals and Drug Delivery Systems
Sharifian, S., & Homaei, A. A. (2022) · Springer eBooks · doi:10.1007/978-981-16-4787-1_12 · Cited by 8 · see citing works
Immunomodulatory and Therapeutic Potential of Marine Flora Products in the Treatment of Cancer
Singh, A., & Krishna, S. (2019) · Springer eBooks · doi:10.1007/978-981-13-7607-8_7 · Cited by 6 · see citing works
Kokoulin, M. S., Savicheva, Y. V., Filshtein, A. P., Romanenko, L. A., & Isaeva, M. P. (2025) · Marine Drugs · doi:10.3390/md23010029 · Cited by 5 · see citing works
Kokoulin, M. S., Savicheva, Y. V., Otstavnykh, N. Y., Kurilenko, V. V., Meleshko, D., & Isaeva, M. P. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms252312927 · Cited by 3 · see citing works
Research Progress on Bioactive Metabolites from Marine Microorganism and Their Structures
Wang, X., Wu, W., Zhi-Hua, C. H. E. N., ZHANG, J., & Bao, B. (2010) · Chinese Journal of Natural Medicines · doi:10.3724/sp.j.1009.2010.00309 · Cited by 3 · see citing works
Non-Pharmaceutical Interventions in Cancer Therapy
Tyagi, V., Tanwar, N., Kumar, N., Phogat, N., Kumar, P., & Kumar, V. (2024) · Current Cancer Therapy Reviews · doi:10.2174/0115733947268161240404084729 · Cited by 2 · see citing works
Marine Polysaccharides and Their Importance for Human Health
Laurienzo, P. (2018) · Blue Biotechnology · doi:10.1002/9783527801718.ch15 · Cited by 2 · see citing works
Kornprobst, J. (2014) · Wiley eBooks · doi:10.1002/9783527335855.marprod015 · Cited by 1 · see citing works
(2011) · SpringerReference · doi:10.1007/springerreference_3636 · Cited by 1 · see citing works
Advances in the Bio-Active Products from Marine Bacteria—A Review
徐, 佰. (2012) · Bioprocess · doi:10.12677/bp.2012.21002
Ahn, G., Lee, W., Kim, K., Lee, J., Heo, S., Kang, N., Lee, S., Ahn, C., & Jeon, Y. (2015) · EXCLI journal · doi:10.17179/excli2014-676 · Cited by 32 · see citing works
Campa‐Córdova, Á. I., González-Ocampo, H. A., Luna‐González, A., Mazón‐Suástegui, J. M., Ascencio, F., & Act, A. (2009)
Duttaroy, A. K. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-822405-2.00027-x
- Authors
- Matsuda, M., Yamori, T., Naitoh, M., & Okutani, K.
- Affiliations
- Kagawa University (JP)
- Japanese Foundation For Cancer Research (JP)
- The University of Tokyo (JP)
- Corresponding author
- Okutani, Koichi
- Venue
- Marine Biotechnology
- Published
- 2003-02-01
- Volume
- 5
- Issue
- 1
- Pages
- 13-19
- ISSN
- 1436-2228 (ISSN-L)
- 1436-2236
- OpenAlex
- W2008562292
- Cited by
- 55
- References
- 12
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 1 · 2022: 3 · 2021: 5 · 2020: 6 · 2019: 2 · 2018: 5 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 3 · 2013: 3 · 2012: 2
- FWCI
- 2.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Marine Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Marine Sponges and Natural Products
- Keywords
- repeating unit, cytotoxicity, anticancer activity, cancer cell line sensitivity, central nervous system cancer, lung cancer, apoptosis, U937 cells, DNA fragmentation, marine natural products
- MeSH terms
- Antineoplastic Agents, Cell Division, Drug Screening Assays, Antitumor, Humans, Neoplasms, Polysaccharides, Bacterial, Protein Conformation, Pseudomonas, Tumor Cells, Cultured, Apoptosis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
