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Cited by 67Seaweed-derived Bioactive CompoundsPhytochemical Studies and BioactivitiesMedicinal Plant Research

The effect of ultrasonificated extracts of spirulina maxima on the anticancer activity

Oh, Sung-Ho, Ahn, Juhee, Kang, Do-Hyung, Lee, Hyeon-Yong·2011·Marine Biotechnology
DOI 10.1007/s10126-010-9282-2
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Abstract

The effect of ultrasonic extraction on extraction yields, cytotoxicity, and anticancer activity of Spirulina maxima was investigated in this study. Optimal extraction conditions were determined as 60 kHz frequency at 60°C for 30 min with 120 W intensity, which resulted in 19.3% of extraction yields and 19.1% of cytotoxicity on normal human cells. Yields from conventional water and ethanol extraction were 15.8% at 100°C and 8.3% at 80°C, respectively. It was found that the extracts obtained by ultrasonic extraction process selectively inhibited the digestive-related cancer cell lines, such as human stomach cancer cells, having 89% of the highest inhibition ratio and 4.5 of the highest selectivity. In adding 0.5 mg/mL of the extract, human promyelocytic leukemia cells' cell differentiation was increased 1.72 times over that of the control. Expression level of B cell lymphoma-2 from Hep3B cell was also effectively suppressed by the extract obtained at 60 kHz and 60°C, leading to the inhibition of the early step of carcinogenesis. This work suggests that anticancer activity of the extracts is due to water-soluble polysaccharides rather than proteins and is further supported by the result that the ultrasonification extraction process can efficiently extract relatively intact polysaccharides rather than digesting the proteins in S. maxima by matrix assisted laser desorption ionization-time of flight and high performance size exclusion chromatography chromatogram analyses. Therefore, ultrasonic extraction increases both extraction yield and the biological activity of S. maxima extracts, which might be useful as an alternative natural anticancer agent in the medical and food industries.

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  • UAE operates with low temperatures, preserving thermolabile compounds and preventing damage to structure; it uses low amounts of solvent and reduced working time, making it fast and inexpensive compared to traditional methods.

    “UAE operates with low temperatures which enables preservation of thermolabile compounds and prevents completely damage of the structure [48]. Low amounts of solvent are used and the working time of extraction is reduced, which makes UAE a fast, inexpensive method as compared to traditional methods [33].”

    From Overview on the Application of Modern Methods for the Extraction of Bioactive Compounds from Marine Macroalgae (2018) · cites this paper as [48]

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Authors
Oh, S.-H., Ahn, J., Kang, D.-H., & Lee, H.-Y.
Affiliations
  • Kangwon National University (KR)
  • Korea Institute of Ocean Science and Technology (KR)
Corresponding author
Lee, Hyeon-Yong
ORCID
Venue
Marine Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2010-04-20
Volume
13
Issue
2
Pages
205-214
ISSN
  • 1436-2228 (ISSN-L)
  • 1436-2236
DOI
10.1007/s10126-010-9282-2
OpenAlex
W1986385242
Cited by
67
References
37
Citations by year
2026: 3 · 2025: 2 · 2024: 3 · 2023: 4 · 2022: 4 · 2021: 6 · 2020: 3 · 2019: 5 · 2018: 14 · 2017: 1 · 2016: 5 · 2015: 2 · 2014: 5 · 2013: 6 · 2012: 2
FWCI
4.90
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Marine Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
Keywords
Spirulina maxima, ultrasound-assisted extraction, anticancer activity, extraction yield, cytotoxicity, promyelocytic leukemia, cell differentiation, Bcl-2 expression, water-soluble polysaccharides, extraction optimization, anticancer natural products
MeSH terms
Ethanol, Antineoplastic Agents, Biological Products, Cell Differentiation, Cytotoxins, Drug Screening Assays, Antitumor, Growth Inhibitors, Humans, Temperature, High-Energy Shock Waves, Cell Line, Tumor, Spirulina

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