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Experimental investigation of collagen waviness and orientation in the arterial adventitia using confocal laser scanning microscopy

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Rezakhaniha, R., Agianniotis, A., Schrauwen, J. T. C., Griffa, A., Sage, D., Bouten, C. V. C., van de Vosse, F. N., Unser, M., Stergiopulos, N.·2012·Biomechanics and Modeling in Mechanobiology
DOI 10.1007/s10237-011-0325-z
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Abstract

Mechanical properties of the adventitia are largely determined by the organization of collagen fibers. Measurements on the waviness and orientation of collagen, particularly at the zero-stress state, are necessary to relate the structural organization of collagen to the mechanical response of the adventitia. Using the fluorescence collagen marker CNA38-OG488 and confocal laser scanning microscopy, we imaged collagen fibers in the adventitia of rabbit common carotid arteries ex vivo. The arteries were cut open along their longitudinal axes to get the zero-stress state. We used semi-manual and automatic techniques to measure parameters related to the waviness and orientation of fibers. Our results showed that the straightness parameter (defined as the ratio between the distances of endpoints of a fiber to its length) was distributed with a beta distribution (mean value 0.72, variance 0.028) and did not depend on the mean angle orientation of fibers. Local angular density distributions revealed four axially symmetric families of fibers with mean directions of 0°, 90°, 43° and -43°, with respect to the axial direction of the artery, and corresponding circular standard deviations of 40°, 47°, 37° and 37°. The distribution of local orientations was shifted to the circumferential direction when measured in arteries at the zero-load state (intact), as compared to arteries at the zero-stress state (cut-open). Information on collagen fiber waviness and orientation, such as obtained in this study, could be used to develop structural models of the adventitia, providing better means for analyzing and understanding the mechanical properties of vascular wall.

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Metadata

Authors
Rezakhaniha, R., Agianniotis, A., Schrauwen, J. T. C., Griffa, A., Sage, D., Bouten, C. V. C., van de Vosse, F. N., Unser, M., & Stergiopulos, N.
Venue
Biomechanics and Modeling in Mechanobiology
Publisher
Springer Science and Business Media LLC
Published
2011-07-10
Volume
11
Issue
3-4
Pages
461-473
ISSN
  • 1617-7940 (ISSN-L)
  • 1617-7959
DOI
10.1007/s10237-011-0325-z
OpenAlex
W2127722806
Cited by
1,096
References
1
FWCI
16.41
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Experimental investigation of collagen waviness and orientation in the arterial adventitia using confocal laser scanning microscopy. https://app.cochat.ai/papers/10.1007/s10237-011-0325-z
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Rezakhaniha, R., Agianniotis, A., Schrauwen, J. T. C., Griffa, A., Sage, D., Bouten, C. V. C., van de Vosse, F. N., Unser, M., & Stergiopulos, N. (2012). Experimental investigation of collagen waviness and orientation in the arterial adventitia using confocal laser scanning microscopy. Biomechanics and Modeling in Mechanobiology. https://doi.org/10.1007/s10237-011-0325-z

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